In tumor cells obtained from patients with Burkittlymphoma, a cancer of the immune system’s B cells,the myc gene often appears close to one of the breakpoints of a reciprocal translocation between chromosomes 8 and 14. In this translocated position, myc is expressed at a higher-than-normal level. Scientists hypothesize that Myc protein overexpression in B cellscontributes to lymphoma formation.a. Explain how transgenic mice produced using pronuclear injection could be used to test this hypothesis. (Assume that you previously cloned a generegulatory region that is active specifically in Bcells throughout the life of the mouse.)b. Suppose you wanted to overexpress Myc only inthe immune cells of mice, starting at one week ofage. To restrict Myc transcription spatially, youwill use same promoter described in part (a). Torestrict Myc transcription temporally, you will usea cre transgene whose expression is controlled byheat shock (hs-cre). Describe the mouse you wouldcreate to accomplish this goal and how you wouldgenerate this mouse. (Hint: What would happenduring Cre-mediated recombination between twoloxP sites in the same DNA molecule and in opposite orientations with respect to each other?)
In tumor cells obtained from patients with Burkitt
lymphoma, a cancer of the immune system’s B cells,
the myc gene often appears close to one of the breakpoints of a reciprocal translocation between chromosomes 8 and 14. In this translocated position, myc is expressed at a higher-than-normal level. Scientists hypothesize that Myc protein overexpression in B cells
contributes to lymphoma formation.
a. Explain how transgenic mice produced using pronuclear injection could be used to test this hypothesis. (Assume that you previously cloned a gene
regulatory region that is active specifically in B
cells throughout the life of the mouse.)
b. Suppose you wanted to overexpress Myc only in
the immune cells of mice, starting at one week of
age. To restrict Myc transcription spatially, you
will use same promoter described in part (a). To
restrict Myc transcription temporally, you will use
a cre transgene whose expression is controlled by
heat shock (hs-cre). Describe the mouse you would
create to accomplish this goal and how you would
generate this mouse. (Hint: What would happen
during Cre-mediated recombination between two
loxP sites in the same DNA molecule and in opposite orientations with respect to each other?)
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