What is the principle of the Restriction Fragment Length Polymorphism (RFLP) in the diagnosis of human diseases? O a. PCR product of a gene is different from the expected one b. The size of a recombinant DNA is different from the expected one OC. The size of a band digested by specific restriction enzymes is different from the expected one O d. The DNA band detected by Southern blot is different from that by Northern blot
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- All are true for DNA polymerase EXCEPT: / Almal geld vir DNA-polimerase, BEHALWE: A. generates dsDNA from SSDNA / genereer dsDNA vanaf SSDNA B. requires a primer with a free 5'- OH end, but the 3'-end may be phosphorylated / benodig 'n voorvoerder met 'n vrye 5'-OH-groep, maar die 3'-einde kan gefosforileer word. C. copies the sequence of nucleotides of one strand in a complementary fashion. / kopieer die volgorde van nukleotiede van een streng komplementêr. D. synthesizes new strands by adding successive nucleotides in the 5'→3' direction. / sintetiseer nuwe stringe deur opeenvolgende nukleotiede in die 5 '→ 3' rigting toe te voeg. E. copies the sequence of nucleotides of one strand to form a new second strand. / kopieer die volgorde van nukleotiede van eenKha Vu Danels Include: 8DX : Safehy Jor bromie tnto lab repart! Name Section date sheet MAPPING PRACTICE #1 Below is a restriction map for the plasmid PGEN 101 (total length = 20 Kb). Using this map as a guide, give the number of restriction fraqments along with their associated lengths that would result from digesting PGEN 101 with the restriction enzymes EcoRI, BamHI and a combination of ECORI and BamHI. BamHI 3.2 Kb 1.7 Kb EcoRI BamHI PGEN 101 8.7 Kb 5.5 Kb .9 Kb EcoRI ECORI DIGESTION PERFORMED SIZES OF FRAGMENTS OBTAINED 10.4 kb , 0.9kb, 8.7 Kb EcoRI 3.2 Kb, 16. 8kb BamHI EcoRI + BamHIConsider a partial restriction digestion, in which genomic DNA is exposed to a small, limiting amount ofa restriction enzyme for a very short period of time.a. Would the resultant fragments be longer or shorteror the same size as those produced by a completedigestion?b. If you prepared genomic DNA from a tissue sample containing millions of cells, would the fragments produced by partial digestion of DNA fromall of these cells be the same or different?
- Consider a partial restriction digestion, in which genomic DNA is exposed to a small, limiting amount ofa restriction enzyme for a very short period of time.a. Would the resultant fragments be longer or shorteror the same size as those produced by a completedigestion?Competent E. coli cells were transformed with the pGLO plasmid. These transformed cells were then allowed to grow on two different plates: 1) a plate containing LB/AMP and 2) another plate containing LB/AMP/ARA. In which plate would you observe both phenotypic and genotypic changes? Briefly justify your answer. Edit View Insert Format Tools Table 12pt v Paragraph v BIUA e T?v 田 D2A linear DNA fragment and a plasmid has three restriction sites for EcoRIhow many fragments will be produced from linear DNA and plasmid respectively.
- Describe how certain restriction enzymes generateDNA fragments with sticky ends, while others generateblunt-ended fragments.The Centers for Disease Control and Prevention (CDC) program PulseNet uses genomic fragments that have been generated by restriction endonucleases O pathogen specific primers O PCR combined with restriction fragment length polymorphism (RFLP) analysis O oligonucleotide probes in microarray technologyYou wish to insert a gene in the plasmid below. (Note the neon green lines represent restriction sites.) amp gene BamHI Sacl Saçl EcoRI BamHI 33 BamHI Gene of interest Chromosomal DNA from human cells a) Which restriction enzyme should you use to cleave the plasmid? Explain your answer. b) How would you create a cDNA library for the chromosomal DNA containing the gene of interest? c) The green area on the plasmid is the ampicillin resistance gene. Explain how you would use it to screen for a recombinant plasmid. 1
- Which of the following sequences in a bacterium CRISPR locus will vary and change O a. Spacer DNA sequences O b. Cas 1 gene O C. Cas 9 gene O d. Cas 2 gene e. CRISPR sequences CLEAR MY CHOICE The following techniques are considered in direct mutagenesis except O a. M13 enrichment protocol O b. M13 bacteriophage C. Using Degenerate Oligonucleotide O d. Using PCR Oe. Using plasmid CLEAR MY CHOICE What is the main trigger for the CRISPR system in bacteria O a. Bacteriophages envelop. O b. Bacteriophages Cas 9 enzyme CLEAR MY CHOICEThe diagram below shows that of a plasmid containing genes that confers erythromycin (ErmR) and ampicillin (Amp) resistance to bacteria that expresses these genes. On the outer portion of the diagram are the labels of some of the different restriction sites available for the insertion of foreign DNA. Psti Scal Erm* plas02 EcoRI (4,381bp) Pull EcoR V BamHI Amp Sall 651 The DNA segment that codes for the protein insulin was inserted into the plasmid using the restriction site BamH I. Which of the following is true if bacteria were able to successfully incorporate a plasmid with the insulin gene insert? O The bacteria will not have any resistance to ampicillin or erythromycin. The bacteria will exhibit resistance to both ampicilin and erythromycin. The bacteria will exhibit resistance to ampicili but not to erythromycin. The bacteria will exhibit resistance to erythromycin but not to ampicillin. The bacteria will randomly exhibit resistance to either ampicillin or erythromycin, but not to…This is a restriction map for the 250 base pair plasmid pSage. Restriction sites for the restriction endonuclease Nhel are 7, 69 and 160. What are the sizes of the restriction fragments produced? Check all that apply. p SAGE Nhel 7 250 bp Nhền 160 Nhel 69 62 69 91 160 97