Experiment: DNA Extraction from BananaThe procedures are attached below.Questions:1. What does mashing do to the fruit? And why is it needed to add detergents?2. What do you think the ethanol does? Why can’t we use room temperature ethanol? B. DNA Extraction from Banana1. Put the ½ banana and ¼ cup distilled water in the sealable plastic bag and mash to make yourslurry.2. In a beaker, mix a solution of 1 tsp. shampoo, 2 pinches of salt and 4 tsp. distilled water. Stirthis solution slowly for about a minute until the shampoo dissolves in the water.Add 2 tsp. of the banana slurry to the soap solution and stir for 5 minutes.Make a filtration set-up and pour in the liquid mixture and let it be filtered. Set aside.Fill a test tube with 2 tsp. of cold ethanol. Very slowly, add 2 tsp. of the filtered banana mixtureto make two layers of liquid.6. Let the test tube sit for 2-3 minutes without disturbing the solution. You will see the clear/whiteDNA precipitate into the alcohol layer.7. Keep the DNA, remove it using a bamboo skewer (a twirling motion works best) and place it ina capped tube filled with alcohol. (Optional)3.4.5.

DNA extraction is a method to obtain the purified chunk of DNA molecule from the mixture. It can be…

Experiment: DNA Extraction from Banana The procedures are attached below. Questions: 1. What does mashing do to the fruit? And why is it needed to add detergents? 2. What do you think the ethanol does? Why can’t we use room temperature ethanol?

Experiment: DNA Extraction from Banana The procedures are attached below. Questions: 1. What does mashing do to the fruit? And why is it needed to add detergents? 2. What do you think the ethanol does? Why can’t we use room temperature ethanol?

Concepts of Biology

1st Edition

ISBN:9781938168116

Author:Samantha Fowler, Rebecca Roush, James Wise

Publisher:Samantha Fowler, Rebecca Roush, James Wise

Chapter10: Biotechnology

Section: Chapter Questions

Problem 2RQ: n gel electrophoresis of DNA, the different bands in the final gel form because the DNA molecules...

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Question

Experiment: DNA Extraction from Banana

The procedures are attached below.

Questions:

1. What does mashing do to the fruit? And why is it needed to add detergents?
2. What do you think the ethanol does? Why can’t we use room temperature ethanol?

B. DNA Extraction from Banana
1. Put the ½ banana and ¼ cup distilled water in the sealable plastic bag and mash to make your
slurry.
2. In a beaker, mix a solution of 1 tsp. shampoo, 2 pinches of salt and 4 tsp. distilled water. Stir
this solution slowly for about a minute until the shampoo dissolves in the water.
Add 2 tsp. of the banana slurry to the soap solution and stir for 5 minutes.
Make a filtration set-up and pour in the liquid mixture and let it be filtered. Set aside.
Fill a test tube with 2 tsp. of cold ethanol. Very slowly, add 2 tsp. of the filtered banana mixture
to make two layers of liquid.
6. Let the test tube sit for 2-3 minutes without disturbing the solution. You will see the clear/white
DNA precipitate into the alcohol layer.
7. Keep the DNA, remove it using a bamboo skewer (a twirling motion works best) and place it in
a capped tube filled with alcohol. (Optional)
3.
4.
5.

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