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1. You order two primers from a company that synthesizes oligonucleotides. The primers have the exact same G and A content, but arranged differently. Primer 1 = GAGAGAGAGAGAGAGAGAGA Primer 2 = GGGGGGGGGGAAAAAAAAAA a) Which one would you expect to have the higher melting point? Explain. b) Based on your answer to (a), draw a melting curve for primer 1 (line) and primer 2 (dashed line). Be sure to label the axes, and the Tm. c) What does the Tm represent? Draw what you would expect Primer 2 to look like at the Tm.

1. You order two primers from a company that synthesizes oligonucleotides. The primers have the exact same G and A content, but arranged differently. Primer 1 = GAGAGAGAGAGAGAGAGAGA Primer 2 = GGGGGGGGGGAAAAAAAAAA a) Which one would you expect to have the higher melting point? Explain. b) Based on your answer to (a), draw a melting curve for primer 1 (line) and primer 2 (dashed line). Be sure to label the axes, and the Tm. c) What does the Tm represent? Draw what you would expect Primer 2 to look like at the Tm.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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1. You order two primers from a company that synthesizes oligonucleotides. The primers have the exact same G and A content, but arranged differently. Primer 1 = GAGAGAGAGAGAGAGAGAGA Primer 2 GGGGGGGGGGAAAAAAAAAA = a) Which one would you expect to have the higher melting point? Explain. b) Based on your answer to (a), draw a melting curve for primer 1 (line) and primer 2 (dashed line). Be sure to label the axes, and the Tm. c) What does the Tm represent? Draw what you would expect Primer 2 to look like at the Tm.
Transcribed Image Text:1. You order two primers from a company that synthesizes oligonucleotides. The primers have the exact same G and A content, but arranged differently. Primer 1 = GAGAGAGAGAGAGAGAGAGA Primer 2 GGGGGGGGGGAAAAAAAAAA = a) Which one would you expect to have the higher melting point? Explain. b) Based on your answer to (a), draw a melting curve for primer 1 (line) and primer 2 (dashed line). Be sure to label the axes, and the Tm. c) What does the Tm represent? Draw what you would expect Primer 2 to look like at the Tm.
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