Use the scoring system described to calculate the alignment score for IPNIA A IG D V VAG P VKGIYA V G D V C-GK Scoring system: • Each identity results in +10 points. • Each gap has a penalty of -25 points. score = What is the percent identity (also called percent identity match)? percent identity: %
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- For most cases, a p value of 0.05 is used to determine whether results fit expected values. Is this a magic number, or could p values be set more stringently, say 0.01, or more lax, say 0.10? What effect would these values have on the reliability of the chi-square test?Suppose you use the semi-global Needleman-Wunsch algorithm to alignGAGATAGwithGACwhere match = 1, nonmatch = 0, gap = -1 When you do the backtracking, how many optimal paths will be found?stion 6 of 18 Suppose that a geneticist discovers a new mutation in Drosophila melanogaster that causes the flies to shake and quiver. She calls this mutation quiver, qu, and determines that it is due to an autosomal recessive gene. She wants to determine whether the gene encoding quiver is linked to the recessive gene for vestigial wings, vg. She crosses a fly homozygous for quiver and vestigial traits with a fly homozygous for the wild-type traits, and then uses the resulting F, females in a testcross. She obtains the flies from this testcross. Phenotype Number of flies vg* qu+ 230 vg qu 224 vg qut vg* qu 97 99 Test the hypothesis that the genes quiver and vestigial assort independently by calculating the chi-squared, X², for this hypothesis. Provide the X2 to one decimal place. X2 = Does the X value support the hypothesis that the quiver and vestigial genes assort independently? Why or why not? the partial table of critical values for X2 calculations to test this hypothesis.
- In a Lineweaver-burk plot, the Y-intercept is -1/KM True or FalseInfo:(For Wildtype) Extinction Coefficient BGG: 0.0007 (y = 0.0007x Beer's Law Graph) Extinction Coefficient BSA: 0.0011 (y = 0.0011x Beer's Law Graph) Cell Lysate: ABS: 0.2, BSA Protein: 285.7mM, BGG Protein: 181.8mM Cell Lysate 1 in 5 dilution: ABsorbtion, 0.03, BSA Protein: 214.3mM, BGG Protein: 136.4mM Elution: Abs .03, Protein BSA: 42.9mM, Protein BGG: 27.3mM Elution DIlution 1/5: ABS: 0.005, Protein BSA: 35.7mM, Protein BGG: 22.7mM Problem: 5. Looking at the sequence of the BGG and BSA standards compared to the sequence of MBP-BAP, which ofthe two standards is the better choice for determining the concentration of MBP-BAP. A. What amino acids react with the Bradford reagent? B. Find the % Composition of amino acids that react with Bradford C. Which standard is best for determining the concentration of MBP-BAP? Briefly explain. D. Explain briefly why choosing a specific standard is less important for determining the concentration of proteinin the cell lysate compared to the…The following table shows the distance moved in an SDS-polyacrylamide gel by a series of marker proteins of known relative molecular weight. a. If the tracking dye had moved a distance of 90.0 mm, calculate linear regression parameters such as linearity coefficient (r), the slope (m), and the y-intercept (b). Protein MW (Da Distance moved (mm) Transferrin 78 000 6.0 Bovine serum albumin 66 000 12.5 Ovalbumin 45 000 32.0 54.0 Trypsinogen Myoglobin Lysozyme Cytochrome c 24 000 17 800 69.0 14 300 79.0 12 400 86.5 Proliferating cell nuclear antigen (PCNA) acts as a homotrimer of three identical subunits and helps increase the processivity of leading strand synthesis during DNA replication. It has a molecular weight of 36.0 kDa. Under the reducing conditions of the sample buffer, the disulfide bridges linking the subunits are reduced and thus the monomer chains are seen on the gel. b. Calculate the Rf of the single band of PCNA when subjected to SDS-PAGE. c. Predict the distance (in mm)…
- Explain this statement "Major considerations in dosage form design with example" ? Please briefly explain at your own easy words.Draw both the Michaelis-Menton and Linweaver-Burke plots. Labe Km vmax and 1/2 vMax where applicable. Indicate how changes in the x-intercept and y-intercept of the line-weaver burke plot affects km and vmaxEdio | Calendar X Edio | Student Da x days/1089977/lessons/1533969/variants/2439102/take/10/ A Q TEXT ANSWER The inheritance patterns for some traits in guinea pigs are listed in the table below. 1. Identify the phenotype of a guinea pig with the genotype HhBBrr. 2. Using the allele symbols in the table, identify the genotype of a guinea pig that is recessive for hair length, heterozygous for hair color, and homozygous dominant for hair texture. Trait Dominant Allele Recessive Allele hair short (H) long (h) length hair color black (B) white (b) rough (R) smooth (r) hair texture BIUG X₁ X¹ EEAA H Normal : √x Enter your answer here ŏooooo Questions Answered 中山川 Ω Ο Τ Edio | Calendar X RepostExchange X Practice All Changes Saved Continue
- 5 & :56M ******* 24 DIHYBRID CROSSES DRV 0 Stv T alı A @ zladenA 9160p2-id2 bns obidalbaneoviene da II\ MOD YR 21 $59A ... Create a dihybrid cross and determine the expected phenotypic percentages of the offspring of two corn plants both of which are heterozygous for colour and texture (RrTt X RrTt). Don't forget to include clear let statements, and follow the all six steps taught on solving genetics problems. insig moni nellog: bna. zoomSearch the menus (Alt+/) 75% Normal text Calibri в IUA 11 + ... 1.| 2 | 3 | • 4.| 5 6 8 12. Although Laurlanthalasa, Princess of the Qualinesti elves, is known for riding into battle on a silver dragon, this is not her main form of transportation. She generally travels by way of griffon. Although the allele for a "crown" of feathers around the head is dominant, it is rarely found in the wild. This may be due to the fact that it makes the griffons stand out, thus making them less effective hunters and more visible to their natural enemies, evil chromatic dragons. This scarcity also makes them prized by elven royalty. Lauralanthalasa was given a breeding pair of bald griffons by an admirer who claims that they are both hybrids for the crowned gene. Create a Punnett Square to determine if Lauralanthalasa will have any chance using these two griffins to produce crowned offspring. Alleles (letters) and Phenotypes All Genotype and Phenotype Parent Punnett Square Possibilities Genotypes…Please ASAP. Thank you. The following diagram represents the results of hemoglobin samples of 5 patients (1-5) that were run on an electrophoresis gel at pH 9.2. The first 3 lanes show the control samples, for comparison. Consider Patient 4 and Patient 5. If patient 2 is female and patient 5 is a male, and they conceive a child together, what is the chance that the child will have sickle cell anemia (the disease)? Give your answer as a percentage (%)