Human growth hormone (hGH) activates expression of the PIND gene in NIH3T3 cells by inducing binding of a transcription factor to the promoter region between -385 and -178 base pairs upstream of the transcription start point. To find the exact binding site of the transcription factor an in vivo footprinting experiment is done with DMS, a chemical compound that will enter the cells and cut the DNA at G residues if no transcription factor is bound to the residue. N C hGH Figure 3.1 In vivo footprint of the PIND gene promoter. The DNA sequence is shown next to the footprint. Lane N is a footprint of “naked" DNA. (Purified DNA with no bound proteins). Lane C is a footprint of NIH3T3 cells that do not express PIND. Lane hGH is a footprint of NIH3T3 cells treated with human growth hormone that induces expression of PIND. 1. Why are some bands missing in lane hGH but not in the two other lanes? || || | || SATACGGAAGGTTGCTGCTGTTGTATGGCT

Human growth hormone (hGH) activates expression of the PIND gene in NIH3T3 cells by inducing binding of a transcription factor to the promoter region between -385 and -178 base pairs upstream of the transcription start point. To find the exact binding site of the transcription factor an in vivo footprinting experiment is done with DMS, a chemical compound that will enter the cells and cut the DNA at G residues if no transcription factor is bound to the residue. N C hGH Figure 3.1 In vivo footprint of the PIND gene promoter. The DNA sequence is shown next to the footprint. Lane N is a footprint of “naked" DNA. (Purified DNA with no bound proteins). Lane C is a footprint of NIH3T3 cells that do not express PIND. Lane hGH is a footprint of NIH3T3 cells treated with human growth hormone that induces expression of PIND. 1. Why are some bands missing in lane hGH but not in the two other lanes? || || | || SATACGGAAGGTTGCTGCTGTTGTATGGCT

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter10: From Proteins To Phenotypes

Section: Chapter Questions

Problem 21QP: Transcriptional regulators are proteins that bind to promoters (the 5-flanking regions of genes) to...

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