1. Eukaryotic transcription initiation begins at the TATA site approximately 30 bp upstream of the transcription start site. One subunit of TFIID (Transcription Factor II D) binds at the TATA site. What is this subunit? 2. This initial binding at the TATA site is specific and coincides with the binding of the entire TFIID complex. This results in a massive change of shape to the DNA helix of the promoter region. After TFIID binding and this shape change, another GTF(General Transcription Factor) can bind. What is this next GTF? 3. Next, which GTF binds to a DNA sequence in the promoter region and to the TFIID-promoter complex before the binding of the RNA Pol II-TFIIF complex? 4. Describe how would you redraw Figure 13-16 to reflect the change in the dsDNA helical shape due to the initial binding to the TATA site? 5. What other interactions, required for specific in vivo transcription initiation, would be made easier by the shape change caused by TATA site binding of the TFIID subunit in question 1? (Use Figure 13-19 as a model) ТВР.TEIIDFIGURE 13-16 Transcription initia-tion by RNA Pol II. The stepwise assemblyof the Pol II preinitiation complex is shownhere and described in detail in the text.Once assembled at the promoter, Pol IIleaves the preinitiation complex upon addi-tion of the nucleotide precursors requiredfor RNA synthesis and after phosphorylationof serine resides within the enzyme's "tail."The tail contains multiple repeats of the hep-tapeptide sequence: Tyr-Ser-Pro-Thr-Ser-Pro-Ser (see Fig. 13-21).TATA boxIFIIATEIIBTFIIF RNA polymerase II4tailSTEIE(TFIIHPreinitiation complex ->ATP DNA meltingOpen complexATPONPromoter escapeInitiation gereFIGURE 13-19 Assembly of the pre-initiation complex in the presence of Me-diator, nucleosome modifiers and remod-elers, and transcriptional activators. In ad-dition to the general transcription factorsshown in Figure 13-16, transcriptional acti-vators bound to sites near the gene recruitnucleosome-modifying and -remodelingcomplexes and the Mediator complex,which together help form the preinitiationcomplex.activator-chromatinremodelerMediator complexHATRIVA polymerase I)

This questions are about transcription.

1. Eukaryotic transcription initiation begins at the TATA site approximately 30 bp upstream of the transcription start site. One subunit of TFIID (Transcription Factor II D) binds at the TATA site. What is this subunit? 2. This initial binding at the TATA site is specific and coincides with the binding of the entire TFIID complex. This results in a massive change of shape to the DNA helix of the promoter region. After TFIID binding and this shape change, another GTF(General Transcription Factor) can bind. What is this next GTF? 3. Next, which GTF binds to a DNA sequence in the promoter region and to the TFIID-promoter complex before the binding of the RNA Pol II-TFIIF complex? 4. Describe how would you redraw Figure 13-16 to reflect the change in the dsDNA helical shape due to the initial binding to the TATA site? 5. What other interactions, required for specific in vivo transcription initiation, would be made easier by the shape change caused by TATA site binding of the TFIID subunit in question 1? (Use Figure 13-19 as a model)

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter31: Completing The Protein Life Cycle: Folding, Processing, And Degradation

Section: Chapter Questions

Problem 16P

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Question

1. Eukaryotic transcription initiation begins at the TATA site approximately 30 bp upstream of the transcription start site. One subunit of TFIID (Transcription Factor II D) binds at the TATA site. What is this subunit?

2. This initial binding at the TATA site is specific and coincides with the binding of the entire TFIID complex. This results in a massive change of shape to the DNA helix of the promoter region. After TFIID binding and this shape change, another GTF(General Transcription Factor) can bind. What is this next GTF?

3. Next, which GTF binds to a DNA sequence in the promoter region and to the TFIID-promoter complex before the binding of the RNA Pol II-TFIIF complex?

4. Describe how would you redraw Figure 13-16 to reflect the change in the dsDNA helical shape due to the initial binding to the TATA site?

5. What other interactions, required for specific in vivo transcription initiation, would be made easier by the shape change caused by TATA site binding of the TFIID subunit in question 1? (Use Figure 13-19 as a model)

ТВР.
TEIID
FIGURE 13-16 Transcription initia-
tion by RNA Pol II. The stepwise assembly
of the Pol II preinitiation complex is shown
here and described in detail in the text.
Once assembled at the promoter, Pol II
leaves the preinitiation complex upon addi-
tion of the nucleotide precursors required
for RNA synthesis and after phosphorylation
of serine resides within the enzyme's "tail."
The tail contains multiple repeats of the hep-
tapeptide sequence: Tyr-Ser-Pro-Thr-Ser-
Pro-Ser (see Fig. 13-21).
TATA box
IFIIA
TEIIB
TFIIF RNA polymerase II
4
tail
STEIE
(TFIIH
Preinitiation complex ->
ATP DNA melting
Open complex
ATP
ON
Promoter escape
Initiation

gere
FIGURE 13-19 Assembly of the pre-
initiation complex in the presence of Me-
diator, nucleosome modifiers and remod-
elers, and transcriptional activators. In ad-
dition to the general transcription factors
shown in Figure 13-16, transcriptional acti-
vators bound to sites near the gene recruit
nucleosome-modifying and -remodeling
complexes and the Mediator complex,
which together help form the preinitiation
complex.
activator-
chromatin
remodeler
Mediator complex
HAT
RIVA polymerase I)

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