1 the overall structure of DNA is double helical and the bases between each strand form watson- crick base pairs. why does RNA make such different structure give atleast two examples. 2Draw a generic triacylgylcerol. Use R for the alkyl groups
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1 the overall structure of DNA is double helical and the bases between each strand form watson- crick base pairs. why does RNA make such different structure give atleast two examples.
2Draw a generic triacylgylcerol. Use R for the alkyl groups.
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- N. NH 2. One of the key pieces of information that Watson and Crick used in determining the secondary structure of DNA came from experiments done by E. Chargaff, in which he studied the nucleotide composition of DNA from many different species. O=P-OCH, N. `NH, HN он O= P- OCH, NH, Chargaff noted that the molar quantity of A_was always approximately equal to the molar quantity of T. and the molar quantity of C was always approximately equal to the molar quantity of G. How were Chargaff's results explained by the structural model of DNA proposed by Watson and Crick? N OH N. O= P-OCH, OH OH2A Draw the complete structure of the trinucleotide 5'-A-G-C-3' (DNA).34. Draw double-stranded DNA (two basepairs long with one AT basepair and one GC basepair). Your drawing should be flat; do not draw the twist of the helix. Be sure to include the 5’-P, 3’- OH, deoxyribose sugar rings (with carbons 1’-5’ and the O indicated), and a simplified phosphodiester bond (O-P-O). Show purines as two differently-sized rings and pyrimidines as one ring and show the correct number of hydrogen bonds between bases. The dsDNA must be antiparallel. Drawing a simplified version of dsDNA will help you gain a better understanding of the concepts underlying dsDNA structure. It will most likely take a couple of tries to get a clear figure.
- The following is diagram of a generalized tetranucleotide. Carbons exist at corners on the shapes and phosphate groups are filled circles A. Suppose that one of the precursors for the tetranucleotide in the diagram was a 32P-labeled guanine nucleoside triphosphate (the innermost phosphate containing the radioactive phosphorus). Where is the radioactive phosphorus atom (i.e., one of the solid black balls) as it exists in the tetranucleotide B. If spleen diesterase (which induces breaks between the phosphate and the 5’ carbon) is used to digest the pictured tetranucleotide, state which base(s) among the breakdown products will be expected to be attached to the 32P.团 X .. Structure and Nomenclature How many molecules of water were formed from the complex of the giver structure? Give ONLY the NUMBER of water molecules. Name the first nucleotide from the 3' to 5' end. Use the correct/appropriate punctuations, spacing and letter case based on the nomenclature. NH, N. to OP-0 OP- -0 O OH NH: NH; -0- NH NH, OP-O- OH OH 0P- 0 0=P-0- OH OH 1:52 pm O Type here to search 4) ENG 昆 11/10/2021r this oligonucleotide, ● Classify if RNA or DNA? Justify your choice. determine the sequence of this segment, labeling the 5' and 3' ends. 2. One of the key pieces of information that Watson and Crick used in determining the secondary structure of DNA came from experiments done by E. Chargaff, in which he studied the nucleotide composition of DNA from many different species. Chargaff noted that the molar quantity of A was always approximately equal to the molar quantity of T, and the molar quantity of C was always approximately equal to the molar quantity of G. How were Chargaff's results explained by the structural model of DNA proposed by Watson and Crick? O=P-OCH, O OH O=P- OCH, 0 O HN OH O=P-OCH₂ 0 NH NH₂ NH₂ 00 OH OH
- Draw the complete structure of the trinucleotide deoxyribo-oligonucleotide that is complementary to 5'dTdGdA3'. Draw this completely, showing all atoms of all parts of the structure.5’ - A T G G C C C A A C T G A C C - 3’ a. How many nucleotides are listed here b. How many codons are listed here c. What are the three structural components of one nucleotide D.Write the appropriate sequence for the complementary strand above or below the sequence shown. Be sure to include which end of the complementary strand is 5’ and which end is 3 E.If the above sequence is the coding strand, write the RNA strand that will be transcribedFor each polypeptide derived in the following sequences: 5' CAA GAG GUA UCC UAC AGA 3' 5' GUC AUC UGG AGG GGC AUU 3' 5' CUA UGC AGU AGG ACA CCC 3' 1. Draw the structure of each polypeptide. 2. Label the amide bonds. 3. Identify the N-terminal and C-terminal amino acids. 4. Write the name of each polypeptide.
- What is the ratio [A¯]/[HA] at pH 2.75? The pKa of formic acid (methanoic acid, H-COOH) is 3.75. Draw the structure of HA for formic acid. Select Draw Templates More C O H Erase [A] [HA] = Draw the structure of the conjugate base for formic acid. Select Draw Templates More с O H Erase困 STRUCTURE AND NOMENCLATURE Name the fourth nucleotide from the 3' to 5' end. Use the How many terminal phosphate/s is/are present in the given structure? Give correct/appropriate punctuations, spacing and letter case based on the nomenclature. ONLY the NUMBER of the terminal phosphate/s. NH, O P-0 OP-O- OH 0=-0- NH: NH: NH; NH. 0=P-0- OH 0P-0- 0P-0- 0=P-0- OH 9:53 am P Type here to search 4) ENG 13/10/2021 近Each group will be provided with 20 g double-stranded DNA oligomer in STE buffer (0.1M NaCl/ Tris/ 10 mM EDTA, pH 7.4). The sequence of one strand of the oligomer is: 5’-AATGGTACGTATACTTTAT-3’ - show a table of % denaturation of DNA at different temperatures, using the equation provided in “Calculation” - plot a graph of % denaturation of DNA vs different temperatures with clear label and title, and hence determine the experimental Tm of the DNA sample - calculate the theoretical Tm of the sample using Marmur and Doty equation, compare the experimental Tm with theoretical Tm of the DNA sample