Genetic Analysis: An Integrated Approach (3rd Edition)
Genetic Analysis: An Integrated Approach (3rd Edition)
3rd Edition
ISBN: 9780134605173
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Chapter 15, Problem 21P

You have isolated another cDNA clone of the CRABS CLAW gene from a cDNA library constructed in the vector shown in Problem 20 . The cDNA was directionally cloned using the EcoR I and Xho I sites. You sequence the recombinant plasmid using primers complementary to the T 7 and T 3 promoter sites flanking the MCS (the positions of these sequences are shown in the figure in Problem 20 ). The first 30 to 60 bases of sequence are usually discarded since they tend to contain errors.

Which of the sequence shown below represents the 5 end of the gene? Which sequence represents the 3 end of the gene?

Will the long stretch of T residues in the T 3 sequence exist in the genomic sequence of the gene?

Chapter 15, Problem 21P, 15.21 You have isolated another cDNA clone of the CRABS CLAW gene from a cDNA library constructed in , example  1

Chapter 15, Problem 21P, 15.21 You have isolated another cDNA clone of the CRABS CLAW gene from a cDNA library constructed in , example  2

Can you identify which sequences are derived from the vector (specifically the MCS) and which sequences are derived from the cDNA clone?

Can you identify the start of the coding region in the 5 end of the gene? What does the sequence preceding the start codon represent?

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Chapter 15 Solutions

Genetic Analysis: An Integrated Approach (3rd Edition)

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