QUESTION 9In our walk-through of the HIV ELISA in zoom class, we were doing a test used to diagnose HIV. How would the procedure be different if we were testing for antibodyto West Nile virus (WNV)?WNV antigen would be adsorbed to the wellsa different enzyme would be attached to the secondary antibodyⒸanti-WNV antibodies would be adsorbed to the wellsenzyme-linked anti-WNV antibody would be used as the secondary antibodyO a different substrate would be used

ELISA stands for Enzyme Linked Immunosorbent Assay. It is a common laboratory method in molecular…

Answered: QUESTION 9 In our walk-through of the…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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QUESTION 5You are asked to recommend an antibiotic to treat potential pathogenicityt in an animal. Describe a method which will allow you to make this recommendation.
QUESTION 5 Based on the plasmid shown, what will you have to use to to distinguish and isolate the bacteria that successfully took up the plasmid? O Lactose O Tetracycline O Ampicillin O UV light to identify the glowing colonies O Xgal
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question: Can you summarize and explain for me what you want to tell in the article below? When I read it myself, I do not understand exactly what is meant by the article. It would be nice if you could highlight the important points. You can use them in a figure or diagram to explain. thank you and hava a nice day :) Article: Nanomaterial-Based Vaccine Development and Immunomodulation Following the publication of the genetic sequence of SARS-CoV-2 on January 11, 2020, intense research efforts have been devoted to developing a vaccine against COVID-19. With unprecedented speed, this extraordinary scientific mobilization led the first vaccine candidate to enter the Phase I human clinical trial on March 16, 2020, and other novel candidates are rapidly following. Up to May 22, 2020, there are 10 COVID-19 candidate vaccines in clinical evaluations and 114 in preclinical development. Concerning vaccine and immunization research, nanomaterials can assist in multiple ways to boost the…
Question 15 How did you select and grow a streptomycin resistant strain of bacteria in this experiment? 1. A sample of bacteria was exposed to streptomycin by inoculating it onto a streptomycin negative plate. Colonies that grew carried a mutation for resistance 2. A colony of bacteria was with streptomycin so that the antibiotic could alter then genetic composition of the bacteria . 3. A sample of bacteria was exposed to streptomycin by inoculating it onto a streptomycin positive plate. Colonies that grew carried a mutation for resistance
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Question 15 How did you select and grow a streptomycin resistant strain of bacteria in this experiment? 1. A sample of bacteria was exposed to streptomycin by inoculating it onto a streptomycin negative plate. Colonies that grew carried a mutation for resistance 2. A colony of bacteria was with streptomycin so that the antibiotic could alter then genetic composition of the bacteria . 3. A sample of bacteria was exposed to streptomycin by inoculating it onto a streptomycin positive plate. Colonies that grew carried a mutation for resistance 4. Samples of bacteria were taken from the culture and observed under a microscope for signs of susceptibility or resistance . Those that were resistant were separated and plated .
QUESTION 4 After you get your gene block, you do a restriction enzyme digest and ligation reaction with the plasmid/gene, so you can proceed to bacterial transformation! How does the plasmid get into the bacteria though? O You mix the plasmid in lipids with several other plasmids to make lentiviral vector that'll infect all the other cells. O The plasmid will be transmitted through a pilus to the other bacteria. O You make the bacteria chemically competent using calcium chloride, and then subject the cells to heat shock O Bacteria will naturally take up the plasmid so you don't need to worry about it.
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