2. You made a pH=9.8 buffer solution by mixing NaOH and glycine to give a solution that is 0.20 M in total concentration of glycine. (pkal-2.34 and pka2=9.69) a) What 2 glycine species are present in the buffer solution at this pH? (draw their structures) b) What is the concentration ratio of these 2 species? c) How many moles of each species, A and HA, is there in 500 ml of the buffer solution. d) If 25 ml of 0.82 M HCl is added to 500 ml of this buffer solution. What reaction is taking place? What is the final pH of the solution? Is the solution still a buffer? e) Draw the titration curve, pH versus equivalents of OH-, for glycine and determine the pl.

2. You made a pH=9.8 buffer solution by mixing NaOH and glycine to give a solution that is 0.20 M in total concentration of glycine. (pkal-2.34 and pka2=9.69) a) What 2 glycine species are present in the buffer solution at this pH? (draw their structures) b) What is the concentration ratio of these 2 species? c) How many moles of each species, A and HA, is there in 500 ml of the buffer solution. d) If 25 ml of 0.82 M HCl is added to 500 ml of this buffer solution. What reaction is taking place? What is the final pH of the solution? Is the solution still a buffer? e) Draw the titration curve, pH versus equivalents of OH-, for glycine and determine the pl.

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

See similar textbooks

Similar questions

2. You made a pH=2.5 buffer solution by mixing NaOH and glycine to give a solution that is 0.20 M in total concentration of glycine. (pka1=2.34 and pka2=9.69) a) What 2 glycine species are present in the buffer solution at this pH? (draw their structures) b) What is the concentration ratio of these 2 species? c) How many moles of each species, A- and HA, is there in 500 ml of the buffer solution. d) If 25 ml of 0.82 M HCI is added to 500 ml of this buffer solution. What reaction is taking place? What is the final pH of the solution? Is the solution still a buffer? e) Draw the titration curve, pH versus equivalents of OH-, for glycine and determine the pl.
the pH of a solution is decreased from 9 to 8, it means that the A) concentration of H+ has decreased to one-tenth (1/10) what it was at pH 9. B) concentration of H+ has increased 10-fold (10X) compared to what it was at C) concentration of OH- has increased 10-fold (10X) compared to what it was at D) concentration of OH- has decreased to one-tenth (1/10) what it was at pH 9. E) Both B and D are correct. p
5. We have 100 mL of 5X TBE (Tris/Borate/EDTA) solution and need to make 1 L of 2X solution. Solid compounds also exist for each of these materials. The molecular weight of Tris is 157.60; Borate has molecular weight of 58.80 and EDTA has a molecular weight of 292.44. a) What is the stock concentration? b) What is the final concentration? c) What is the starting volume? d) What is the final volume? e) Write out the equation used to solve this. Show all your work. Are we able to make a solution using this dilution method using are starting materials? If not, propose another way to make the desired concentration and volume of the solution. Show your work.
Calculate the concentration of Y4-ion in 0.0100 M EDTA solution at pH 6. α4 = 2.2 x 10-5 for pH 6.
1.) A sample contains 0.003 mg of NaOH. How many micrograms (μg) is this? 2.) An experiment was performed in which 400 mL of 0.2 M urea was used. Calculate the number of millimoles (mmol) of urea contained in this sample. 3.) How many micromoles (μmol) of ATP are present in 4 mL of 5 mM ATP solution..
If an aqueous solution has a hydroxyl ion concentration of 1 × 10-6 M, what is the concentration of hydrogen ion ([H+]) ? [H+] = 1.0 x10-5 M
What is the pH of a buffer solution that is 0.20 M proprionic acid (HC3H4O2) and 0.1 M sodium proprionate (NaC3H4O2)? The KA of proprionic acid is 1.3 x 10-5
You have a 1000X stock solution of sodium chloride (NaCl). The concentration of NaCl in the stock solution is 5M. a)How would you make 300mL of a 1X solution? b)What is the concentration of NaCl in your 1X solution?
1) A solution is made by combining 128 mL of ethanol and 0.452 L of water. In this solution, what is the solvent? What is the solute?2) If a solution contains 128 mL of ethanol and water to make 0.452 L of solution, what is the (v/v)% concentration?3) How many grams of sodium carbonate are needed to make 350 mL of a 0.5% (w/v) sodium carbonate solution?4) What is the molarity of a magnesium chloride solution made by dissolving 8.7g of magnesium chloride in 2.45 L of water?5) An exercise in lab requires you to make 500 mL of a 2.3M beryllium sulfide solution. How many moles of beryllium sulfide will you need?6) How many grams of sodium chloride are needed to make 0.60L of a 2.3M sodium chloride solution?7) How hot must the air in a balloon be heated if initially it has a volume of .750 L at 20°C and the final volume be 1.00 L?8) Convert 455 mmHg to atm.9) A container of gas has an initial pressure of 0.8 atm. The pressure is increased to 1.1atm and the final volume of gas is 2.3 liters.…
2. 500 ml of a 0.1 M sodium phosphate buffer with pKa = 7.21, is diluted %3D a) How would you dilute this buffer 100 times to make a final volume of 50 ml? Show the ratios to be added together. b) Calculate the pH of the buffer when it is diluted with dH20 c) Calculate the pH of the buffer when it is diluted with 0.3 M KCI %3D 3. What is the normality of a 5 M phosphoric acid (SG = 1.88 g/cm³; 85% solution has a Mr of 98 g/mole
1. Calculate the pH of a buffer solution made from 0.20 M CH3COOH and 0.50 M CH3COO- that has an acid dissociation constant for CH3COOH of 1.8 x 10-5.
3.) A mixture of amino acids was subjected to paper chromatography with six amino acid standards in order to determine its composition. After visualization using ninhydrin, the measured distances were as follows: A B с D E F G a.) What is the Rf value of each amino acid observed? X Distances: Solvent 10.3cm A 4.50cm B - 1.32cm C - 6.60cm D - 5.20cm E - 3.52cm F 8.70cm G - 10.26cm X1 - 9.72cm X2 - 4.3cm X3 7.1cm - b.) What are the possible components of the amino acid mixture? Answer can be up to two amino acids per component.