Map of pUC18 is shown on the here. Describe how to select recombinant clones if a foreign DNA is inserted in to the polylinker site of pUC18 and then introduced into E. coli cells. Hindll Sphl Sbfl Pstl Acci Hincll Sall Xbal BamHI Aval Smal Xmal Acc651 Kpnl Banll Eco53kl Saci Apol ECORI lacz MCS LacR binding site Plac PUC18 Amp 2686 bps PMB1 ori

Map of pUC18 is shown on the here. Describe how to select recombinant clones if a foreign DNA is inserted in to the polylinker site of pUC18 and then introduced into E. coli cells. Hindll Sphl Sbfl Pstl Acci Hincll Sall Xbal BamHI Aval Smal Xmal Acc651 Kpnl Banll Eco53kl Saci Apol ECORI lacz MCS LacR binding site Plac PUC18 Amp 2686 bps PMB1 ori

Name: Map of pUC18 is shown on the here. Describe how to select recombinant
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Description: Map of pUC18 is shown on the here. Describe how to select recombinantclones if a foreign DNA is inserted in to the polylinker site of pUC18 and thenintroduced into E. coli cells..HindllSphlSbflPstlBspMIAcciHincliSallXbalBamHIAvalSmalXmalAcc651KpnlBa

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter28: Dna Metabolism: Replication, Recombination, And Repair

Section: Chapter Questions

Problem 9P: Heteroduplex DNA Formation in Recombination From the information in Figures 28.17 and 28.18, diagram...

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Given the following double-stranded fragment of DNA: 5'- ACTTGGCAGGCCTTCGATCC-3' 3'- TGAАССGTCСGGAAGCTAGG-5' A hypothetical restriction endonuclease recognizes a 6bp sequence with two-fold symmetry (typical for restriction enzymes) found in this fragment and catalyzes cleavage of this DNA on both strands between GG nucleotides within the recognition sequence. This nuclease exhibits b-type cleavage (atypical for restriction enzymes). Draw the double-stranded sequence of each fragment after cleavage showing any phosphates left on the ends.
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