The E coli cell contains 107 non-specific DNA binding sites for R, 10 R molecules, and 1 Operator. And the mechanism of specific binding of R to O works well with these concentrations. Would it work if R = 1? Explain with a calculation.

The E coli cell contains 107 non-specific DNA binding sites for R, 10 R molecules, and 1 Operator. And the mechanism of specific binding of R to O works well with these concentrations. Would it work if R = 1? Explain with a calculation.

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter28: Dna Metabolism: Replication, Recombination, And Repair

Section: Chapter Questions

Problem 21P

See similar textbooks

Related questions

Q: If you were educating expecting parents on pregnancy, labor and delivery, as well as the first year…

A: Pregnancy:Prenatal Development:This section outlines the stages of fetal development during…

Q: GQ13

A: The objective of the question is to understand the role of the mutant MC1R protein in determining…

Q: explain: infectious pathogens distrubtion with the use of articles

A: The objective of this question is to understand how infectious pathogens are distributed and how…

Q: Smudge cells are frequently seen in: Question 6 options: A) CLL.…

A: The objective of the question is to identify the type of leukemia in which smudge cells are…

Q: A country that sees most Hepatitis B (HBV) infections in young adults is most likely to have what…

A: The correct answer is D. High endemicity. Hepatitis B virus (HBV) infections that occur…

Q: If 80% of drunk drivers fail to pass a sobriety test (walking a straight line for 5 meters) in 60…

A: The objective of the question is to determine the specificity and sensitivity of a sobriety test…

Q: Discuss briefly the pharmaceutical applications of micelles?

A: Micelles are self-assembled structures formed by certain molecules called amphiphiles in a suitable…

Q: MAKE A GRAPH FOR ME ON GRAPH PAPER CALL IT ENZYMES VS RATE OF REACTION USING TABLE BELOW GRAPH…

A: Please give me helpful rating if you are satisfied with the graph.

Q: How would most biologists and anthropologists explain the reasons behind why primates do specific…

A: Primate behavior is generally understood by scientists and anthropologists to be the consequence of…

Q: true or false if a restriction enzyme recognizes the restriction site, 5' AACGTT3', and the enzyme…

A: 1. Recognition Site of the Restriction Enzyme:The recognition site of a restriction enzyme is the…

Q: For each of the three dietary macromolecules describe their digestion and and absorption in the body

A: Deeper explanation of how digestion and absorption work for each dietary…

Q: How can we explain that fossils of Mesosaurus, an extinct reptile that could not swim across open…

A: Mesosaurus, a ancient reptile that lived in freshwater amid the early Permian period. Its fossils…

Q: For each of the following, identify whether the statement about Hox genes is true for Drosophila…

A: 1. Hox genes are present in the genome Both Drosophila (fruit flies) and vertebrates have Hox genes…

Q: If 85% of diabetic patients are correctly identified by a urine test for glucose, but 25% of…

A: The objective of the question is to determine the sensitivity and specificity of a urine test for…

Q: der these chemical species by increasing pH of an 0.1 M aqueous solution of each. That is, imagine…

A: Step 1: Step 2: I have given detailed step by step solution approach to solve this problem go…

Q: What is the common ancestor of the Galapagos finches? What are the thirteen Galapagos finches? What…

A: Delving deeper into each aspect related to the Galapagos finches: 1. Common Ancestor: The common…

Q: Please help! Research and describe a rapid antibody test used to detect a disease. Answer the…

A: Name of test: HIV Self-Test (various brands available)There are numerous brands of HIV self-tests…

Q: These types of proteins are responsible for all the following events during cell division: movement…

A: Approach to finding a solution to the problem:1. Determine the most important activities that take…

Q: Why is the control of gene expression important for cells? Choose one: It ensures the accurate…

A: The question is asking us to identify the primary reason why the control of gene expression is…

Q: The diagnostic cell in Hodgkin's disease is the: Question 5 options: A)…

A: The objective of the question is to identify the specific cell type that is characteristic of…

Q: using the method for experiment below and the table conduct 1 graph of the different factors vs rate…

A: Here is a funnel graph of your data and method above:

Q: is no mystery how harmful a lot of environmental factors can be on a fetus nor is it misunderstood…

A: There are different kinds of birth defects which can be induced in the fetus either by some genetic…

Q: GQ14

A: The objective of the question is to understand the meaning of the statement that 'mutations occur…

Q: Which of Aristotle’s four causes is a definition of the substance of which something is composed?…

A: The objective of the question is to identify which of Aristotle's four causes refers to the…

Q: Which of Aristotle’s four causes is a definition of the substance of which something is composed?…

A: The objective of the question is to identify which of Aristotle's four causes refers to the…

Q: Genetics Q4

A: The objective of the question is to identify the tool or method used in gene therapy to cut the DNA…

Q: What is the molecular evidence that natural selection includes the “rejection of injurious change”?

A: Natural selection is a fundamental concept in evolutionary science, portrayed by Charles Darwin as…

Q: 25

A: The objective of the question is to understand the effect of a drug that inhibits DNA methylation on…

Q: Define about Whole-Exome Sequencing ?

A: Whole- exome sequencing( WES) is a genomic approach for sequencing all of the protein- encoding…

Q: What kind of dentition do strepsirhines have? What kind of food do they eat and how do their teeth…

A: Approach to solving the question:1. Define strepsirhines and their dentition.2. Discuss their…

Q: 17

A: The question is asking about the possible outcomes of alternate splicing of a gene that has 6 exons.…

Q: GQ16

A: The objective of the question is to identify the correct statement about the biological process of…

Q: Anaerobic respiration requires great stores of glycogen requires extensive capillaries for oxygen…

A: The question is asking about the requirements and role of anaerobic respiration in human energy…

Q: 32. The antimetabolites inhibit: a) the ribosome assembly b) the ribosome movement c) cell…

A: Certainly! Let's delve into the details of how antimetabolites inhibit glycolysis and the Krebs…

Q: Which of the following trees correctly shows the relationships between alpha- proteobacteria (a…

A: The correct relationship between the mentioned entities would be:Bacteria and Archaea are separate…

Q: Replication Methods and Central Dogma1. Information about replication2. What happens at each step

A: DNA replication is a crucial process in biology, essential for the accurate transmission of genetic…

Q: how infectious pathogens are distributed , give 5 examples

A: The objective of this question is to understand how infectious pathogens are distributed and to…

Q: Suggest the most appropriate organic/aqueous medium for use in determining P values in the following…

A: Partition coefficients (P values) are vital in understanding how a medication or chemical carries on…

Q: There are thousands of children born every year with genitalia structures that are nol fully male or…

A: The objective of this question is to understand the biological reasons behind the occurrence of a…

Q: What is the difference between ventilation and respiration?

A: Ventilation:Ventilation is the process of moving air in and out of the lungs. It involves the…

Q: A nurse asks, "What is the difference between alcoholic hepatitis and alcoholic cirrhosis?"

A: Hepatitis is defined as a disease whereby the liver is inflamed that can be brought on by many…

Q: What kind of eyes do strepsirhines have? How can you tell what species are active during night vs…

A: Approach to Solving the Question:1. **Research on Strepsirhines Eyes**: Firstly, I would delve into…

Q: N 3 13 Which of the following lipid is likely to function as membrane lipid in mammals. O…

A: The objective of the question is to identify the lipid that is likely to function as a membrane…

Q: Calculate the 2 possible values of x in this equation: x2 – 16x + 48 = 0, either by using the…

A: The objective of this question is to find the two possible values of x in the given quadratic…

Q: Name three advantages of seeds over spores in terms of the probability that the next sporophyte…

A: Probability is the term used to portray the probability that some occasion will happen.…

Q: Which of the following is NOT an adaptation among plants to increase access to nitrogen?…

A: Mutualistic association with fungi that convert atmospheric nitrogen into a biologically available…

Q: -Centromere D D Ø ☹ A B ℗ ℗ D E C

A: The correct chromosomal condition for one daughter nucleus at telophase of mitosis is diagram E.…

Q: Genetics Q6

A: The question is asking where a tRNA molecule carrying the first methionine (MET) would be located in…

Q: Genetics Q8

A: The objective of the question is to identify which region of the mRNA, when affected by triplet…

Q: Explain why pyrimidine is less basic than pyridine

A: The objective of the question is to understand why pyrimidine is less basic than pyridine. This can…

Concept explainers

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.

Question

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

SEE SOLUTION Check out a sample Q&A here

Step 1: Introduction of the topic

VIEW

Step 2: Understanding binding of a regulatory protein to DNA

VIEW

Solution

VIEW

Step by step

Solved in 3 steps with 1 images

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.

Similar questions

Consider a mutant form of the lac repressor protein whose ΔGo for binding DNA differs from that of the wild-type protein by 2 kcal/mol, the approximate free energy equivalent of a single hydrogen bond. What is the difference (i.e., the ratio) of the Kd values (for DNA binding) of the wild-type versus the mutant lac repressor protein?
(c) By binding one L-tryptophan molecule/monomer, the trp repressor binds to DNA to suppress syn- thesis of L-tryptophan in E. coli. Below is the amino acid sequence of the helix – (reverse) turn – helix region of the trp repressor that binds to DNA compared to the sequence of the corresponding DNA binding motif of the Prl protein, a different type of repressor protein. A diagram of the trp repressor dimer is also shown. reverse turn trp helix 4 70 Trp -Gly-Glu-Met-Ser-Gln-Arg-Glu-Leu-Lys-Asn-Glu-Leu-Gly-Ala-Gly- Ile- Prl -Ser-Glu-Glu-Ala-Lys-Glu-Glu-Leu-Ala-Lys-Lys-Cys-Gly-Ile-Thr- Val- Pri heilix trp helix 5 80 90 Trp Ala-Thr-Ile-Thr-Arg-Gly-Ser sgn-Ser-Leu-Lys-Ala-Ala- Prl Ser-Gln-Val-Ser-Asn-Trp-Phe-Gly-Asn-Lys-Arg-Ile-Arg- Prl helix
Consider the mechanism of the enzyme RNase:  What would happen to the Km (i.e., would it increase, decrease, or stay the same) if the his12 was mutated to a lysine? Explain.  What would happen to the Kcat (i.e., would it increase, decrease, or stay the same) if the his12 was mutated to a valine? Explain.
Many blood clotting proteins undergo a post-translational modification in which specific glutamic acid residues (Glu) in the protein are converted to gamma-carboxyglutamic acid residues (Gla). See reaction scheme below. An example is the blood clotting protein Factor IX, which has 12 Glu in its N-terminus converted to Gla. This modification gives Factor IX the ability to bind calcium and phospholipid membranes. Bacteria do not have the enzyme required to convert Glu to Gla and therefore Factor IX proteins expressed in bacteria would not have the proper modifications. How might you engineer the translational apparatus of a bacterial cell line so that it produces Factor IX with Gla in the appropriate positions. How would you ensure that only the 12 Glu in Factor IX that are normally converted to Gla and not just all Glu (Limit 5-6 senetnces)?
In Figure 12-26, provide a biochemical mechanism forwhy HP-1 can bind to the DNA only on the left side of thebarrier insulator. Similarly, why can HMTase bind onlyto the DNA on the left of the barrier insulator?
The distal Histidine (His 64) in myoglobin is subjected to three different mutations, this is one of them: H64N. (Histidine to Aparagine) For the mutation, draw a theoretical binding curve and CO relative to the O2 and CO binding curves for wild-type Mb (see example below). Provide a clear rationale for the binding curve.
Consider the following simple regulatory pathways. Assume the full pathway is shown. A- E- B- F- C- G- D- 1 A H- 2 B || L You identify several null mutations (a complete deletion of the gene). For each mutant (indicated with a - sign), determine whether the final product (I, J, K or L) is inducible, uninducible, or constitutive. 3 C 4 D- [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] E [Choose ] F G I H || J K
Calculate the concentration of lac repressor, assuming that one molecule is present per cell. Assume that each E. coli cell has a volume of 10-¹² cm³ . Would you expect the single molecule to be free or bound to DNA?
CTP synthetase catalyzes the glutamine-dependent conversion of UTP to CTP. The enzyme is allosterically inhibited by the product, CTP. Mammalian cells defective in this allosteric inhibition are found to have a complex phenotype: They require thymidine in the growth medium, they have unbalanced nucleotide pools, and they have an elevated spontaneous mutation rate. Explain the likely basis for these observations.
You are studying how your Lys-Val-Thr tripep de interacts with another pep de, which places an Asp in close proximity to the Lys on your pep de. How would the presence of an Asp side chain affect the pKa of the Lys side chain? Briefly explain your reasoning. (remember pKas are rela ve, pKa=log([H+][A-]/[HA])
(a) You have found a new, enhanced version of the drug jafrasitor, (called jafrasitorplus) (molecular weight 600 grams/mol), that binds the histone deacetylase enzyme Sir2 with a dissociation constant KD = 0.03 nM. What mass of jafrasitorplus should be administered to a patient with a blood volume of 5.5 L such that Sir2 is at least 91% inhibited? Show your work and reasoning.
Consider the structure of Cro repressor protein from bacteriophage lambda E. It is a DNA binding protein, and like many sequence- specific DNA binding proteins, it must function as a homodimer Ex. Notice the mutual docking of a phenylalanine residue from each subunit into a hydrophobic pocket of the partner subunit. These hydrophobic interactions are required for dimerization. The noncovalent interactions highlighted in yellow are also required for dimerization. These interactions represent examples of: Osecondary structure O tertiary structure O quaternary structure O secondary AND quaternary structure Ⓒ tertiary AND quaternary structure