Image 1 shows raw data of gel electrophoresis. Label/annotate image 1 (lanes, ladder sizes, etc). Explain what your seeing in the gel. Use the following information and picture 2 to assist in labelling. The purpose of this gel electrophorsis is to ensure that your GOI, FAP257, is in each BAC. Protocol that was done for Gel electrophoresis: -Place tray with gel into gel box -Fill gel box with IX TAE until the gel is completely submerged -Remove the comb for wells -Load 10ul of the 1kb Gene Ruler ladder (well 1) -Contains DNA ladder, 6X TriTrack DNA, Loading Dye, and Deionized water -Load other wells -Well 2: Control -Well 3: BAC- 15M5 -Well 4: BAC-39K10 -Well 5: BAC-27N17 -Run the gel at 100V for 30 minutes or until the dye front has migrated 2/3 down the gel
Image 1 shows raw data of gel electrophoresis. Label/annotate image 1 (lanes, ladder sizes, etc). Explain what your seeing in the gel. Use the following information and picture 2 to assist in labelling. The purpose of this gel electrophorsis is to ensure that your GOI, FAP257, is in each BAC. Protocol that was done for Gel electrophoresis: -Place tray with gel into gel box -Fill gel box with IX TAE until the gel is completely submerged -Remove the comb for wells -Load 10ul of the 1kb Gene Ruler ladder (well 1) -Contains DNA ladder, 6X TriTrack DNA, Loading Dye, and Deionized water -Load other wells -Well 2: Control -Well 3: BAC- 15M5 -Well 4: BAC-39K10 -Well 5: BAC-27N17 -Run the gel at 100V for 30 minutes or until the dye front has migrated 2/3 down the gel
Medical Terminology for Health Professions, Spiral bound Version (MindTap Course List)
8th Edition
ISBN:9781305634350
Author:Ann Ehrlich, Carol L. Schroeder, Laura Ehrlich, Katrina A. Schroeder
Publisher:Ann Ehrlich, Carol L. Schroeder, Laura Ehrlich, Katrina A. Schroeder
Chapter6: The Lymphatic And Immune Systems
Section: Chapter Questions
Problem 91LE: tonsils and _____________________________
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Image 1 shows raw data of gel electrophoresis. Label/annotate image 1 (lanes, ladder sizes, etc). Explain what your seeing in the gel. Use the following information and picture 2 to assist in labelling.
The purpose of this gel electrophorsis is to ensure that your GOI, FAP257, is in each BAC.
Protocol that was done for Gel electrophoresis:
-Place tray with gel into gel box
-Fill gel box with IX TAE until the gel is completely submerged
-Remove the comb for wells
-Load 10ul of the 1kb Gene Ruler ladder (well 1)
-Contains DNA ladder, 6X TriTrack DNA, Loading Dye, and Deionized water
-Load other wells
-Well 2: Control
-Well 3: BAC- 15M5
-Well 4: BAC-39K10
-Well 5: BAC-27N17
-Run the gel at 100V for 30 minutes or until the dye front has migrated 2/3 down the gel
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