Your friend hasn't been feeling well, and you think that she should get tested because it could be a virus or even multiple viruses! Nancy is conducting a qPCR experiment todetect how much of virus A, B, and/or C your friend is infected with. All three viruses contain RNA as their genetic material. Nancy takes a saliva sample and is excited to begin.However, it's her very first time conducting qPCR so a few mishaps occurred during this experiment.Unfortunately...●Nancy forgets to reverse transcribe the RNA of virus A, and• The cDNA of virus C contains a mutation where the probe is designed to bind, and• Nancy uses the same color fluorescent dye for viruses B and CFortunately, all the probes consisted of a fluorescent and quenching dye. Which qPCR graph is most representative of Nancy's experiment after several cycles of qPCR? Explainwhy the answer you chose is correct.A.(₁FluorescenceBB# of CyclesAA# of CyclesсB.D.FluorescenceB# of CyclesB# of Cyclesс

PCR or polymerase chain reaction is a lab procedure that amplifies fragments of DNA or RNA by using…

Answered: Your friend hasn't been feeling well,…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Lab, you will need to design your own experiment that detects the nucleic acids from the given list of virus genes. This experiment's results could be used to identify the viruses present based on the genes and nucleic acids. Virus gene list • NP gene from different avian influenza virus (AIV) subtypes • H5 subtype of AIV • H7 subtype of AIV • H9 subtype of AIV Based on the above list of virus genes, design an experiment that will differentiate the genes based on the molecular weights of the different nucleic acids present. • What method will you use and why? • What specific % agarose will you use for this experiment? • How will the listed genes separate (which will be at the top and which at the bottom)? Include a drawing that shows the differentiated nucleic acid patterns you would expect to see as the results of your experiment. This pattern can be hand-drawn or
you want to design a vaccine that contains DNA that can fight against new variant (MU) of COVID-19 strain. You know that you can alter the information in DNA and can make a viral-vector based vaccine. What kind of virus you will use and why? not a writing assignment just few sentences
Remdesivir is an antiviral drug used to treat SARS-CoV-2 infections. It is a broad-spectrum antiviral, meaning it has activity against many different viruses. Remdesivir can be incorporated into a new RNA chain as a virus replicates and can interfere with the viral replication machinery. Template RNA Elongation New RNA SARS-CoV-2 RNA polymerase Template groove Nucleotide entrance Nucleotides Remdesivir Figure 1. A representation of remdesivir and the viral replication machinery for SARS-CoV-2. 6. Figure 1 shows that remdesivir "mimics” an important component of RNA replication. Which specific component of RNA replication has a structure like that of remdesivir? 7. Propose a hypothesis about how remdesivir might inhibit the virus's replication process.
A disgruntled scholar recently tried to infect their instructor with what appeared the GVV synthetically derived. Symptoms of infection include occasional random outbursts and occasionally the urge to randomly chuck bottles of condiments when things aren't going your way. None of those symptoms are present yet in the instructor, but the instructor is nonetheless worried based on the phenotype of this very large and intimidating virus. The instructor decides to investigate the genome of this unknown virus to confirm if they should be worried and be on the lookout for symptoms. Which method would be most appropriate to determine the virus genome to see if it in fact is an infectious variant of GVV? gel electrophoresis reverse genetics the polymerase chain reaction (PCR) whole genome shotgun sequencing metagenomics cloning
answer doesn't have to be long i just need an explanation, thank you! The whole world right now is concerned (or they should be) about the appearance of new variants of SARS-CoV-2. Among other things, these variant viruses have mutations that change the amino acid sequence of the spike protein on the virus surface. While this is concerning, I would like to propose that there is a limit to how far these mutations can change the spike protein. Why can't there be an endless number of new mutations of this viral protein? How would these amino acid changes affect how the virus works?
Someone testing for the presence of a virus like COVID often has very little genetic material to analyze. This might include the genetic material from only a few viruses. However, a person with even a few viruses in them would still be infected, might be contagious, and could still spread the virus to others. How does a scientist detect and then amplify the very small amounts of genetic material that might be found on a nasal swab, such as is used for COVID testing? O RFLPS O Gel electrophoresis PCR O STRS
All viral genomes contain nucleic acid, either DNA or RNA but not both. Viral replication is the formation of biological viruses during the infection process in the target host cell. Viruses must first enter the host cell before viral replication can occur. After gaining entry into a cell, the viral genome has to be first replicated using either the host DNA polymerase or RNA polymerase You are studying two viruses: Virus A is an enveloped RNA virus Virus B is non-enveloped DNA virus Which of the above viruses is likely to have the lowest mutation rate in the host cell? O Virus A because it uses the host RNA polymerase which has lower error rate than DNA polymerase. O Virus A because it uses the host DNA polymerase which has lower error rate than RNA polymerase. O Virus B because it uses the host DNA polymerase which has lower error rate than RNA polymerase. O Virus B because it uses the host RNA polymerase which has lower error rate than DNA polymerase.
You have two cell lines named ViRa and SaSi growing in your lab. ViRa is susceptible to poliovirus infection, but is not permissive. SaSi is not susceptible to poliovirus infection, but is permissive. Is infectious virus produced after infection of ViRa cells with poliovirus? No Is infectious virus produced after infection of SaSi cells with poliovirus? No Is infectious virus produced after transfection of ViRa cells with poliovirus RNA? Yes Is infectious virus produced after transfection of SaSi cells with poliovirus RNA? Yes
What do you think is the reason why this virus undergoes mutation?
Answer the following 1.Are viruses living or non-living organisms? 2. What is the difference between HIV and AIDS? 3. HIV is classified as a retrovirus. What are retroviruses? 4. In your own words, provide a short summary of the HIV replication cycle. 5. Why is reverse transcriptase an important enzyme in the replication cycle of HIV? 6. In the chapter about enzymes, we have discussed how drugs are basically inhibitors. There are currently a multitude of Antiretroviral therapy (ART or ARV) options available to people living with HIV (PLHIV) so that the viral replication is suppressed and does not progress to AIDS. What do you think these drugs target? Discuss briefly. 7. SARS-CoV-2, the virus that causes CoVid-19, belongs to the Coronaviridae family. Viruses under this family have a genome made of single-stranded positive-sense RNA. Is this similar to the viral genome of HIV? If so, do you think that – in theory – they would have similar replication cycles? 8. In your own opinion, do…
What enzyme is required for the movement of transposable elements that involve an RNA intermediate in virus? options: RNA polymerase reverse transcriptase polyA polymerase peptidyl transferase
Viruses are host-specific, meaning they can only infect specific types of host organisms. For examble, the adenovirus is responsible for the common cold in humans but your dog can't catch a cold from you. Why is this? The proteins on the virus surface can only bind with proteins on the correct host surface. It is an enveloped or non-enveloped virus. The enzymes carried by the virus are compatible with the host. The genome of the virus is made of DNA or RNA.

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