Q: how does the diameter of the column affect the flow rate of the elution in gel filtration…
A: Introduction Gel Filtration Chromatography IsA Type Of Size Exclusion Chromatography, Which Can Be…
Q: You aseptically transfer 1 mL of your original liquid culture into 99 mL of sterile water. What is…
A: The dilution factor is a term used to describe the ratio of the final volume over the aliquot…
Q: ow many mL of 3% sodium chloride will be needed to complete the following compound? Sodium…
A: To calculate the ml of 3% sodium chloride -' Mass of Sodium bicarbonate - 4.2 gm = 4200 mg Sodium…
Q: What is the mechanism of the reaction of the Bradford reagent with proteins? (explain in not less…
A: The Bradford protein assay is used to measure the concentration of total protein in a sample.
Q: 4 mL of 10% TCA solution was added to 1 mL of serum and after mixing, it was waited for two minutes…
A: Calibration graphs are used to estimate the concentration of unknown sample by plotting it against…
Q: What is the difference of measuring proteins through UV-VIS spectroscopy at 280 nm and at 595 nm…
A: The proteins estimation is a routine process when working with different proteins. The protein…
Q: When combining 0.5 mL of Lysozyme solution (with a concentration of 10 mg/ml) and 0.5 mL of water,…
A: Lysozyme is an enzyme which catalyzes the hydrolysis of peptidoglycans present in cell walls of…
Q: Prepare a standard tube that contains 0.2 mL of 2.5 mM lysine. To this tube, add 0.1 mL of the…
A: Final concentration can be calculated from the formula C1V1=C2V2 Where C1= initial concentration V1=…
Q: You receive an order for “heparin 6,000 units SQ q 12 hrs”. The stock solution contains heparin…
A: A drug, sometimes called medicine or medication, is a chemical molecule that, when ingested, has a…
Q: epare 100 cfu/mL bacterial suspension. Present in diagram if necessary and show all calculations…
A: A colony-forming unit, or CFU, is a measurement of microbe concentration in a test sample. To get a…
Q: From this standard curve and chart below, does the separation of molecules in the mixture appear…
A: Size exclusion chromatography is a technique used to separate molecules by the virtue of their…
Q: Why does the nitrate reduction tube turn red after the addition of zinc?
A: Nitrate reduction test is used to find the bacteria who have the ability to reduce the nitrate.
Q: Given that the vol of blood taken is 20uL, and the vol of the diluting fluid is 5.0ml. Calculate the…
A: A hemoglobin test measures the amount of hemoglobin in your blood. Hemoglobin is a protein in your…
Q: How many grams each of dextrose and sodium chloride are used to prepare a 500mL bag of D5 1⁄2NS for…
A: Intra Venous Infusions --Intravenous injections are used for emergency purposes when pt. is not in a…
Q: more than one dosage strength is available, choose the most appropriat Ordered: Furosemide oral…
A: Medication administration is always followed by dosage regimen and accurate dosage calculations.…
Q: p please. MD ordered morphine SO4 gr 2/5 and the pharmacist has a stock solution of gr 1/8 per…
A: As we know Morphine has analgesic properties and is used to relieve pain. The drug belongs to a…
Q: Infuse heparin at 1,200 units per hour from a solution containing 40,000 units of heparin in 500 mL…
A: Introduction:- Heparin is an anticoagulant medication. It is a naturally occuring glycosaminoglycan…
Q: If each agar plate contained 20 ml of LB agar and had an ampicillin concentration of 150 ug/ml, what…
A: Given data: Each plate contains- LB agar=20ml Ampicillin= 150 micrograms per ml. Amount of…
Q: Describe the appearance of the protein solutions in each of the tubes after addition of the…
A: Proteins are complex nitrogen-containing organic molecules that involve all aspects of the live…
Q: Calculate the elution volume of blue dextran (blue), myoglobin (red), and Bromocresol purple…
A: Chromatography is separation method of mixture of compounds using column, paper etc, that is based…
Q: How does gel filtration work? What are the applications of analytical versus preparative versions…
A: Chromatography is a technique for separating a mixture in the laboratory. The mixture is dissolved…
Q: What is Zeta Sedimentation Ratio?
A: As per our answering policy, we can only answer one question at a time. Please post the other…
Q: For solid dosage form what are the SOP that we must follow during dispensing process?
A: Solid dosage form includes tablets and capsules. SOP (Standard Operating Procedure) is a collection…
Q: What is the concentration of a 50% solution after completing three 1:10 serial dilutions?
A: Serial dilution is the process that reduces the concentration of a solution in an organised manner.…
Q: Using 25 μL of serum, how much diluent would be needed to create a 1:2 dilution? ______ μL
A: The process of reducing a solute concentration by adding solvent to a solution is referred to as…
Q: What is the basic principle of GPC (gel permeation chromatography)?
A: Gel permeation chromatography is a type of size-exclusion chromatography that uses organic solvents…
Q: For a TLC experiment, what is the purpose of the pentane extraction of spinach juice? Why wouldn’t…
A: Answer: Introduction:Thin layer chromatography, or TLC, is an analytical method to analyse mixtures…
Q: How much NS was diluted? b. How much Gentamicin was diluted? c what is the desired concentration?
A: Question is related to drug calculation. Available gentamicin dose is - 20 mg/ 2 ml Desired dose is…
Q: A solution of flycine and a solution of albumin were prepared with the same concentration (g/L). One…
A: The ninhydrin test is a chemical that is used to determine the presence of alpha amino groups in the…
Q: 100ml of LB media with 25 μg/ml of Amp and 100 μg/ml of Kan final concentration. You have 100ml of…
A: An artificial nutrient medium contains nutritional components like sugars, salts, amino acids, etc.…
Q: How much serum is needed to create 2μL of a 1:20 dilution
A: Question is related to drug calculation . Detailed solution in step 2.
Q: . How many ml of amoxicillin will you add to your mini bag? 2. Calculate drip rate per minute for…
A: 1 gram of amoxicillin is diluted in 10 ml. Dose is 250 mg in 100 ml NS. So, 1000/250 is total of 4…
Q: Outline in detail the procedure for celloidin embedding .
A: Embedding is that the method during which the tissues or the specimens are capsulate in a very mass…
Q: Explain this approach: matrix-assisted laser desorption ionization (MALDI) ?
A: MALDI is the technique used to detect the mixture of organic molecules like synthetic biopolymers.…
Q: What is the principle involved in the Dinitro salicylic (DNS) colorimetric method?
A: DNSA refers to 3, 5-dinitrosalicylic acid. It is an aromatic compound. It is widely used in…
Q: Explain how to prepare 250 ml liquid and 500 ml solid form separately from LB (Luria Bertani) medium…
A: The LB agar medium is designed in such a way that it is nutrient rich which supports the growth of…
Q: With the aid of a diagram, explain the principles of gel filtration chromatography.
A: Gel filtration chromatography: Chromatography is an analytical technique where the components of a…
Q: CFU/mL
A: in calculation via conventional formula , CFUs/ml in 2nd dilution are greater than 1st dilution…
Q: You have an order for 1 gram of Cefazolin in D5W 100 ml. You have added 5 ml of sterile water to the…
A: Introduction:- In this problem the dilution of the drug is decreasing like recommended is 10ml…
Q: What is the structural and functional differences between Inoculating loop versus inoculating…
A: Inoculating tools These tools are use by microbiologist to inoculate microorganisms easier.
Q: Calculate the amount of casein obteined from %20 milk solution with %2 HCl isoelectric point…
A: Protein precipitation is widely used in downstream processing of biological products in order to…
Q: The original concentration in a sample of kombucha is 2.79 x 10^6 CFU/ml. Which dilution would yield…
A: Formula, Original cell count = #colonies / (volume plated × dilution) Viable plate count = 30 - 300
Q: How much serum is needed to create 2 µL of a 1:20 dilution?
A: Drug computations practice will help caregivers build better and more assured arithmetic abilities.…
Q: Differentiate thymolphthalein monophosphate method from alpha naphthyl phosphate method of prostatic…
A: Prostatic acid phosphatase: It is a glycoprotein synthesized by the prostate gland that is capable…
Q: 1. What is your total dilution factor? 2. What is the molar concentration of the dye in the original…
A: Absorption spectroscopy is a spectroscopic technique in which we measure the absorption of radiation…
Q: After trypsinization of cells in the flask and cell counting, you determined a concentration of 10…
A: The detachment of cells from the adhering substrate is essential for specific cellular…
Q: A patient requires 2200 kcal/day from D50 (50% dextrose) and 8.5% aa (initial concentration). What…
A: TPN means Total Parentral Nutrition is a method of feeding in which fluids are given into a vein…
Q: In what order would the following proteins eluted from a gel filtration column of Sephadex G200:…
A: Chromatography is a method of separation of specific compound from the mixture of compounds. This…
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
- Infuse heparin at 1,200 units per hour from a solution containing 40,000 units of heparin in 500 mL D5W. How many mL/hr will deliver the ordered dosage?Calculate the volume of BSA stock that will be required to make the standard solutions needed to create the BSA standard curve. Be sure to show your work and include the volume of 0.02 M phosphate buffer required to reach a final volume of 1 mL. From a 2,000 μg/mL BSA stock, create 1 mL of each of the following stock solutions in 0.02 M phosphate buffer using individual microcentrifuge tubes: 50 μg/mL, 250 μg/mL, 500 μg/mL, 1,000 μg/mL, 1,250 μg/mL, 1,500 μg/mL. Be sure to properly label all the microcentrifuge tubes before creating the standards.Calculate the elution volume of blue dextran (blue), myoglobin (red), and Bromocresol purple (purple) in the gel filtration chromatography below with a flow rate of 2.5 mL/min. (Take note that the black colored test tubes represent colorless eluents)
- Paclitaxel is used to treat ovarian cancer by infusion at a concentration of 0.6 mg/mL over 24 hours. A dose of 135 mg/m² has been prescribed for Sero. Sero is 1.06 m tall and weighs 92 kg. Paclitaxel comes in vials of 150 mg/25 mL. Calculate the volume of 5%w/v glucose solution that would be required to dilute the concentrated solution to prepare an paclitaxel solution of the required strength.Paclitaxel is used to treat ovarian cancer by infusion at a concentration of 0.6 mg/mL over 24 hours. A dose of 135 mg/m² has been prescribed for Sero. Sero is 1.06 m tall and weighs 92 kg. Paclitaxel comes in vials of 150 mg/25 mL. Calculate the volume of 5%w/v glucose solution that would be required to dilute the concentrated solution to prepare an paclitaxel solution of the required strength. Answer: You did not give the correct unit. The correct answer is: 333 mL XApple puree was analyzed for petulin by HPLC-MS-MS after SPE clean-up. The procedure was 10.0g of puree + 10μl of a 10μg/ml solution of isotopically labeled petulin as internal standard were treated with 10.0ml of pectinase and acetic acid, centrifuged, and filtered. Four ml of the filtrate was passed through a SPE cartridge. The petulin was eluted with 2.0ml of ethyl acetate. The sample was evaporated to dryness and the residue dissolved in 1.0ml of the mobile phase. The analyte signal was 127 and the internal standard 197. Calculate the concentration of petulin in the sample in μg/g (RRF=1).
- DILUTION COLONY COUNT CFU/mL 1:106 155000 1:107 15500 1:108 1550 1:109 155 Given these values how would I fill in the rest of this serial dilution table? Also, what would be a 1:1 CFU/mL value based on this table?Streptomycin sulfate 400 mg IM daily is ordered for a child weighing 77lbs. The recommended dosage is 20-40 mg/kg/day once daily. A 1 gram vial of streptomycin sulfate is available in powdered form with the following instructions: Dilution with 1.8 mL of sterile water will yield 400mg per mL. If safe, how many mLs will you give?The order is for 1000ml of 5% D/W, 1000ml of Ringers Lactate, 500ml of NS to run over a 36 hour period. The drop factor is 15gtt/ml. How many gtt/min will you administer? using dimensional analysis and show work
- a)Determine the amount of X (in gram) in order to prepare 100 mL of 50 mM stock solution of X. Show your work. (MW of X= 225) b)Determine the volume required (mL) from the stock solution X in (i) to prepare 500 mL of MS medium containing 5 mg/L X. Show your work.KCI for injection contains 40 mEq in 20 mL. An order requires 15 mEq, how many mL are needed?750mL mL of a dextrose solution is to be infused at 45 mL/h starting at 1600 hours. At what time will this IV be completed?