For a TLC experiment, what is the purpose of the pentane extraction of spinach juice? Why wouldn’t we want to spot an aqueous solution onto the silica gel?
Q: You are trying to separate five proteins, which are listed below, by gel filtration chromatography.…
A: Chromatography is a laboratory technique that helps to separate and identify components present in a…
Q: Why is the order of separation based on size opposite for gel filtration and gel electrophoresis,…
A: Gel filtration and gel electrophoresis are biochemical techniques used for the separation of…
Q: What is the advantage of the doing cell wall isolation, determining composition by the use of Matrix…
A: All plant cells are enclosed by complex walls that play a role in tissue growth and differentiation.…
Q: In lab two different mobile phase compositions (acetone and hexanes) were used for column…
A: Thin layer chromatography (TLC) is a form of adsorption chromatography in which the separation of…
Q: What is covalently attached to the resin beads for us to purify GST-DHFR-His by IMAC? Group of…
A: Answer given below..
Q: Which of the following tools cannot facilitate the visualization of specific intracellular proteins…
A: Radio isotopes can visualise uptake and downtake but not specific intra cellular proteins. DNA…
Q: When eluting your analyte of interest using HPLC, gradient elution can be used. How does Gradient…
A: HPLC is expanded as High Performance Liquid Chromatography. It helps in excluding out the analyte in…
Q: What is the purpose of the pentane extraction of spinach juice? Why wouldn’t we want to spot an…
A: Thin layer chromatography, or TLC, is an analytical method to analyse mixtures by separating the…
Q: how many ml of sterile water injection must be mixed with two 4 ml vials of sterile hypertonic NaCl…
A: Tonicity refers to the ability of a solvent to cause cells to shrink or swell. A semipermeable…
Q: (MB = methylene blue) What is the concentration of the sample of the substance with the reporter…
A: Here the absorbance of the methylene blue sample is given to be: 0.40 at 595 nm. According to the…
Q: In a hypotonic solution why would potato stick become crispier?
A: A hypotonic solution has fewer dissolved particles (such as salt and other electrolytes) than normal…
Q: Why is agarose an ideal gel matrix for electrophoresis experiments? Because it forms a liquid at all…
A: Nucleic acids are negatively charged molecules because of the phosphate backbone. Due to their…
Q: Why does water separate out from a gel after freezing?
A: On freezing of gel some damage occur to gel. damage occur to polymer and colloids that binds the gel…
Q: how can phosphorylation be detected via 2D gels?
A: 2D gels It refers to the two-dimensional gel electrophoresis. It is generally used to detect and…
Q: In size exclusion chromatography, which component will elute last in the set up? Group of answer…
A: Size exclusion chromatography is also known as gel filtration chromatography (GFC) or gel permeation…
Q: Why are we using this chemicals in the experiments?(RECOGNITION REACTIONS IN CARBOHYDRATES AND…
A: Hi! Thanks for your question. As you have posted multiple questions and have not mentioned which…
Q: How to graph a scatter plot /X-Y plot of ph and absórbance. pH is the independent variable (x-axis)…
A: pH of tube A400 after 5 minutes 3 0.008 5 0.371 7 0.546 9…
Q: You are set to run your first SDS polyacrylamide-gel electrophoresis. You have boiled your samples…
A: SDS-PAGE, which is an abbreviation for sodium dodecyl sulfate-polyacrylamide gel electrophoresis, is…
Q: If you want a better resolution, would you have increased or decreased the agarose percentage in the…
A: The composition of agarose gels has an effect on the migration rates of DNA molecules. As the…
Q: A mixture of dipeptides consisting of Lys-Gly, Thr-His, Ser-Leu, Glu-Gln was applied to column…
A: Ion-exchange chromatography is a type of protein purification technique in which proteins are…
Q: suctose density gradient ultractifugation is a powerful technique for fractionating macromolecules…
A: The sucrose density gradient is used for fractionating the DNA, RNA, and protein. The samples are…
Q: List the five components of isoelectric focusing gel list the five components of isoelectric…
A: ISOELECTRIC FOCUSING(IEF) technique used for the separation of proteins. PRINCIPLE :- It is a…
Q: List three coating techniques used to make sol-gel coatings, and briefly describe each technique.
A: Sol-gel Is a method of creating solid materials from tiny molecules. The process involves the…
Q: What other method could be used to purify lysozyme from white using its unusual pl?
A: The unique charge properties of lysozyme with a very high pI of about 10.5 can be used in the…
Q: Using ultrafiltration, you concentrate a solution of Protein X as follows: Initial volume protein X…
A: Ultrafiltration is a laboratory technique used to remove minute-level impurities from biological…
Q: 50 mg/50 ml kinetin stock is available You need 2.5 mg/l kn for Ms media calculate amount of kn is…
A: A culture medium is a liquid or semi-solid mixture of various nutrients that support microorganism…
Q: Pls explain why Which of the following reagents is CORRECTLY matched to its purpose? a. Coomassie…
A: sandwich affect in Biochemistry is the effect of reactive functional groups or chemical compounds in…
Q: Negatively charged proteins migrate towards the top of the gel backwards towards the positive…
A: The gel electrophoresis is a technique by which different biological samples including DNA, RNA and…
Q: What is the effect of trypsin EDTA solution on monolayer cell culture preparation?
A: Trypsin is an enzyme which helps in digestion of proteins.In the small intestine,proteins are broken…
Q: Why do we use a stacking gel in SDS-PAGE
A: SDS-PAGE is a mostly used gel-electrophoresis for separation of proteins. It is used to separate…
Q: Which among the following demonstrates the difference between Native Polyacrylamide Gel…
A: PAGE or polyacrylamide gel electrophoresis is a separation technique under the influence of…
Q: In extraction of albumin in egg white using 100-ml water, what does albumin look like? Also, what do…
A: In extraction of albumin in egg white and placed in water some reactions are occur..
Q: When run under nondenaturing conditions (without SDS or β-mercaptoethanol), native polyacrylamide…
A: Gel electrophoresis is the technique of separation of molecules on the basis of their size and…
Q: In theory, if a 1-liter culture of rich medium is inoculated with 3 bacterial cells per milliliter,…
A: Generation time is the time taken for the doubling of the single cell. According to data given in…
Q: SEE EXAMPLE IN THE IMAGE A mixture of 0.20M acetic acid and 0.30M sodium acetate is given. Calculate…
A: The Henderson Hasselbalch equation for calculation of the pH of a solution of weak acid and its…
Q: After adding stop solution (sulphuric acid) in reaction mixture of HRP assay with TMB, yellow color…
A: TMB(3,3',5,5'-tetramethylbenzidine) substrate is used for ELISA detection. The blue-colored solution…
Q: Your labmate forgot to use CBB-R250 in the process. Which of the following would most likely be the…
A: The Coomassie brilliant blue is a staining dye used in the laboratory to quantify, visualize the…
Q: With the aid of a diagram, explain the principles of gel filtration chromatography.
A: Gel filtration chromatography: Chromatography is an analytical technique where the components of a…
Q: What is the basic principle of GPC (gel permeation chromatography)
A: Gel permeation chromatography is a simple way to determine the molecular weight of your sample…
Q: You are conducting an assay for glucose, wherein the color change of DNS due to reduction by glucose…
A: A biochemical assay refers to the analytical technique that is used to detect and quantify the…
Q: If you have 4 compounds and you test them for antimicrobial activity using a disk diffusion assay…
A: The disk diffusion assay is a culture based microbiology assay to determine the susceptibility of…
Q: What are the advantages of granulation ? during tablet formulation.
A: Introduction: A tablet formulation commonly contains at least one dynamic substance and other…
Q: Sulfamethoxazole is a type of sulfonamide antibiotic. Its absorbance ranges from 250-300 nm in a UV…
A: Sulfamethoxazole Sulfamethoxazole is a sulfonamide based bacteriostatic antibiotic, widely used to…
Q: How do we ensure that gel electrophoresis is functional? a Running buffer, containing electrolytes,…
A: Electrophoresis can be described as the process that migration and separation of charged particles…
Q: Why does in this experiment, after gel is formed from gelatin the marble in papaya juice to the…
A: Gelatin is a biochemical substance frequently used to form gels. Gelatin is formed by hydrolysis of…
Q: IN A BIURET ASSAY TWO PROTEIN SOLUTION CONTAINING 150MG/ML AND 180MG/ML SHOWS THE SIMILAR…
A: Biuret test of protein is to done to quantify the proteins or to detect the concentration of…
For a TLC experiment, what is the purpose of the pentane extraction of spinach juice? Why wouldn’t we want to spot an aqueous solution onto the silica gel?
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
- In gel electrophoresis and column chromatography, why do large molecules migrate more easily in one method and with more difficulty in the other? Which method generated the most precise results?After adding stop solution (sulphuric acid) in reaction mixture of HRP assay with TMB, yellow color becomes brighter. What is the mechanism?In the assay of citric acid, what do you think about the nature of solution sample ? a) Before titration and b) After titration.
- If you want a better resolution, would you have increased or decreased the agarose percentage in the gel? Would you modify the voltage you run the gel at?Which of the following technique is FALSE in microscopy? Osmium tetroxide can be used as a fixative as well as a negative stain in transmission electron microscopy. Two proteins; each tagged with their individual primary antibody followed by one protein with rhodamine conjugated secondary antibody and the other with fluorescein-conjugated secondary antibody can be visualized simultaneously using a fluorescence microscope. The emission spectrum of Rhodamine is 580 nm The emission spectrum of Fluorescein is 521 nm O An objective lens with 100X magnification and projection lens with 10X magnification is going to produce a 1000X total magnification. Phase contrast and differential interference contrast (DIC) do not require staining.a) What are the important parameters for the eluent selection in Gel Permeation Chromotography (GPC)? Explain your answer in detail. What is an universal calibration curve and what is the advantage of using it in GPC analysis?
- You overexpressed (asked bacteria to make a lot of) an 80 kDa protein carrying a 6X-His tag and purified it on a nickel column. You collect frac ons from your purifica on and analyze them using SDSPAGE. The results are shown in image attached. a) Interpret the results from the gel. What is wrong? (see image attached) b) Identify 2 possible reasons why this problem is occurring, and for each reason, suggest an experimental modifica on you can do to troubleshoot.What side of the electrophoresis chamber (red or black) should the wells be on when the gel is placed in the chamber? What would happen if you placed the agarose gel with the wells on the opposite side of the chamber?Explain the differences between gel electrophoresis and column chromatography. Address the principles behind each separation. Why do large molecules migrate more easily in one method and with more difficulty in the other? Which method generated the most precise results?
- IN A BIURET ASSAY TWO PROTEIN SOLUTION CONTAINING 150MG/ML AND 180MG/ML SHOWS THE SIMILAR ABSORBANCE. IS IT POSSIBLE?Prior to subculture, Tifa used a hemocytometer to count her HepG2 cells cultured on a T-25 flask. After trypsinization, she prepared her cells by mixing 50 µL of the cell suspension with 50 μL of 0.4% trypan blue. Her observation under the microscope is as shown below: 1 (1) (ii) 3 (iii) 2 (iv) 4 Note: Count cells on the four labelled squares. Include cells touching the line on top and left. 18P 06. ¿66 Ⓡ Determine the number of viable and dead cells from her observation. What is the percentage of viability of this culture? Show your calculations in detail. Calculate the concentration of viable cells per mL in the original culture. Show your calculations in detail. Based on your understanding of cell culture, do you think she should proceed with subculture? Justify your answer.Which of the following is true of the gel electrophoresis shown? A) the power source has not been turned on yet. B) the three wells contained the same DNA molecules. C) well #1 had fewer molecules of DNA than well #2 or #3 D) well #3 had more different sized molecules of DNA than well #1 or #2 Which of the following statements is TRUE about the band of molecules labeled X ( close to where it says positive electrode). A) it contains DNA fragments that are shorter than the ones in any of the other bands. B) it contains fewer DNA molecules than any of the other bands from lane 3. C) it contains more negatively charged particles than any other band on the gel. D) it contains fewer genes than any of the other bands on the gel.