With the aid of a diagram, explain the principles of gel filtration chromatography.
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With the aid of a diagram, explain the principles of gel filtration chromatography.
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- briefly explain the principle behind this technique; gel-filtration chromatographyWith the aid of diagrams, explain the processes and principles of each of the following methods: (a) Gel filtration chromatography on Sephadex G50 b.SDS-PAGELabel the void volume (V0), elution volume (Ve) and total elution volume (Vt) appropriately on the graph for the gel filtration chromatography using a column experiment.
- State the functions for each of these components used in the gel filtration chromatography using a column experiment. Bromophenol blue A mixture of albumin and bromophenol blue SpectrophotometerWhat is the basic principle of GPC (gel permeation chromatography)?In Gel filtration chromatography, when will you stop collecting eluents if sample is not colored?
- Explain this approach: matrix-assisted laser desorption ionization (MALDI) ?How does gel filtration work? What are the applications of analytical versus preparative versions of the method ?3) Define gel electrophoresis, including its theory and application. Describe the steps of running gel electrophoresis using the following image. More detailed reading: https://www.sciencedirect.com/topics/medicine-and-dentistry/agar-gel-electrophoresis POWER SUPPLY CATHODE ELECTROPHORETIC BUFFER ANODE WELL SAMPLE AGAROSE GEL POWER SUPPLY CATHODE ANOCE HIGH MOLECULAR WEIGHT SPECIES LOW MOLECULAR WEIGHT ANALYTES
- Explain the differences between gel electrophoresis and column chromatography. Address the principles behind each separation. Why do large molecules migrate more easily in one method and with more difficulty in the other? Which method generated the most precise results?Describe in detail the process of electrospray ionization ?a. Let’s say for example that a milk sample has 10,000 bacteria per milliliters. If 1 mL of this sample were plated out, these would theoretically be 10,000 colonies in the Petri plate. Discuss and explain the serial dilutions of this example. b. The disk diffusion method was used to evaluate 3 disinfectants. The results were as follows: Solution Zone of inhibition X 0 mm Y 5 mm Z 10 mm How would you interpret? Provide inference.
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