Explain how to prepare 250 ml liquid and 500 ml solid form separately from LB (Luria Bertani) medium whose components and concentrations are given below. Yeast extract 5 g/L Peptone 10 g/L NaCl 10 g/L Agar 1.5 g/L
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- The following ingredient list represents which type of growth media? Beef extract 1.5 g Yeast extract 3.0 g Peptone 6.0 g Glucose 1.0 g Agar 15.0 g water 1000 ml pH 6.6Answer the ff with not more than two sentences: Why should Erlenmeyer flasks hold not more than 60% of their capacity? When can gelatin be used in culture media preparation? How does cotton plug prevent contamination of the medium? Why should caps of glassware be loosened first before sterilization?The nutrient broth is a basic media used for growing a broad variety of microorganisms in the laboratory. The nutrient broth consists mainly of 1.5g * L ^ - 1 extract, 3g * L ^ - 1 yeast extract and 5g * L ^ - 1 sodium chloride dissolved in distilled water. The nutrient agar is prepared by adding the agar at the desired amount into nutrient broth. Answer the following questions. marks) a) How would you prepare a 500 ml nutrient broth? b) How would you prepare 2% (w / v) nutrient agar for 1L? c) How would you prepare a 100 ml nutrient broth supplemented with 100mu * g / m * l ampicillin antibiotic? The stock concentration for ampicillin solution is 100mg / m * l .
- What nutrients do the following media components contain? Peptone Yeast extract Beef extract Potato infusion Agar Why should agar media be completely dissolved before they are dispensed in tubes and plates? What are the bases for pegging the temperature at 1210C for 15-30 minutes during moist heat sterilization and 1800C for two (2) hours using dry heat sterilization? 1.Can you sterilize culture media using dry heat sterilization? Why is that so? You will notice in the videos shown, the cotton plug is not used. What is role of cotton plug in media prep, sterilization and culture of microorganisms? Instead of using cotton plug, plastic screw-cap is used, can you substitute this for the former? Is it technically acceptable in microbiology?Please include sourceSuspend 28.0 grams in the 1000 mL distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (1210C) for 15 minutes. Cool to 45-500C. If desired the medium can be enriched with 5-10% blood or other biological fluids. Mix well and pour into sterile Petri dishes. 1. Determine the amount of dehydrated medium needed to prepare 50 nutrient agar plates. Include amount for 2 additional plates as excess to compensate for compounding losses.Nitrate reduction to nitrite is indicated by the formation of a pink to red colour after addition of nitrate test reagent. However, if a culture does not produce a colour change several posibilies exist. Explain.
- Given the following media recipe, pick the terms that apply (pick as many as are applicable). 10.0 g Meat extract 10.0 g Peptone 5.0 g Sodium chloride 15.0 g 1.0 L Agar Water Once media is cool, add 6% sterile sheep's blood Defined Complex Minimal Selective DifferentialWhy would the following medium not be considereda chemically defined medium: glucose, 5 grams (g);NH4Cl, 1 g; KH2PO4, 1 g; MgSO4, 0.3 g; yeast extract, 5 g;distilled water, 1 liter? What is aseptic technique andwhy is it necessary?With the aid of diagrams, explain the processes and principles of each of the following methods: (a) Gel filtration chromatography on Sephadex G50 b.SDS-PAGE
- Why is the colony diameter of the yeast culture grown on potato dextrose agar is larger than those grown on acetate agar? Give and discuss the reasons for this different in growth.Please explain why is 70% ethanol used as an effective disinfecting solution in labs? Why not 95% ethanol? Please include references.Give the components, properties and special considerations in preparation (if there's any) on the following media 1. MacConkey Agar 2. EMB Agar 3. HEA 4. SSA