As described in 4C above, PLP-dependent enzymes can generate formaldehyde from serine. This compound would be toxic if widely distributed in the cell, so normally the formaldehyde is captured by tetrahydrofolate, generating N^5 ,N^10-methylene tetrahydrofolate a) Draw a general acid/general base mechanism that an enzyme might use for this transformation b) Explain the role of the coenzymes N^5 , N^10-methylene tetrahydrofolate, and NADPH in thymidylate synthase, which converts dUMP to dTMP (no mechanism needed; an accurate written description will do,
As described in 4C above, PLP-dependent enzymes can generate formaldehyde from serine. This compound would be toxic if widely distributed in the cell, so normally the formaldehyde is captured by tetrahydrofolate, generating N^5 ,N^10-methylene tetrahydrofolate a) Draw a general acid/general base mechanism that an enzyme might use for this transformation b) Explain the role of the coenzymes N^5 , N^10-methylene tetrahydrofolate, and NADPH in thymidylate synthase, which converts dUMP to dTMP (no mechanism needed; an accurate written description will do,
Chapter1: Numerals And Fractions
Section: Chapter Questions
Problem 5RP
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As described in 4C above, PLP-dependent enzymes can generate formaldehyde from serine. This compound would be toxic if widely distributed in the cell, so normally the formaldehyde is captured by tetrahydrofolate, generating N^5 ,N^10-methylene tetrahydrofolate
a) Draw a general acid/general base mechanism that an enzyme might use for this transformation
b) Explain the role of the coenzymes N^5 , N^10-methylene tetrahydrofolate, and NADPH in thymidylate synthase, which converts dUMP to dTMP (no mechanism needed; an accurate written description will do,
Transcribed Image Text:H₂N N N
HN
N5
NHR
10
tetrahydrofolate (THF)
CH₂O
H₂N .N
HN
!!
N
-NR
+ H₂O
N5, N10-methylene THF
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