Microbiology: An Introduction
12th Edition
ISBN: 9780321929150
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Concept explainers
Videos
Textbook Question
Chapter 28, Problem 1CAE
Suppose you are culturing a microorganism that produces enough lactic acid to kill itself in a few days.
- a. How can the use of a bioreactor help you maintain the culture for weeks or months? The graph below shows conditions in the bioreactor:
- b. If your desired product is a secondary metabolite, when can you begin collecting it?
- c. If your desired product is the cells themselves and you want to maintain a continuous culture, when can you begin harvesting?
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
Suppose that you were tasked with 4 cultures of a specific Bacteria Species in Luria Broth medium: (1) culture A – cells are in lag phase; (2) culture B – cell in log phase; (3) culture C – cells in stationary phase; and culture D – cells in decline phase. Imagine that you observe the growth rates of each culture in a fresh sterilized LB medium. Now, plot the outcome growth curves of cultures A, B, C and D in a single graph.
If a biotechnologist wishes to obtain a primary metabolite, they will harvest the organism/media during log phase. If a biotechnologist wishes to obtain a secondary metabolite, they will harvest the organism/media during stationary phase. Can you explain this distinction regarding the metabolism of the cell at these two different batch culture growth phases?
Based on the growth curve, which ONE of the following best describes what would happen if sample of culture in the presence of drug B is inoculated onto drug-free growth medium?
Select one:
A.
Growth present, but cell count increased by at least 99.9% of the original culture
B.
No growth after 24 hrs
C.
Growth at 24 hrs
D.
Not possible to predict because the culture could either be alive or dead
E.
Growth present, but cell count reduced by at least 99.9% of the original culture
Chapter 28 Solutions
Microbiology: An Introduction
Ch. 28 - What is industrial microbiology? Why is it...Ch. 28 - How does commercial sterilization differ from...Ch. 28 - Why is a can of blackberries preserved by...Ch. 28 - Outline the steps in the production of cheese, and...Ch. 28 - Beer is made with water, malt, and yeast; hops are...Ch. 28 - Why is a bioreactor better than a large flask for...Ch. 28 - The manufacture of paper includes the use of...Ch. 28 - Describe an example of bioconversion. What...Ch. 28 - Prob. 9RCh. 28 - NAME IT Van Leeuwenhoek was the first to see this...
Ch. 28 - Foods packed in plastic for microwaving are a....Ch. 28 - Acetobacter is necessary for only one of the steps...Ch. 28 - Use the following choices to answer questions 35:...Ch. 28 - The spoilage of canned foods caused by Geobacillus...Ch. 28 - A heat-resistant fungus that causes spoilage in...Ch. 28 - The term 12D treatment refers to a. heat treatment...Ch. 28 - Which one of the following is not a fuel produced...Ch. 28 - Which type of radiation is used to preserve foods?...Ch. 28 - Which of the following reactions is undesirable in...Ch. 28 - Which of the following reactions is an oxidation...Ch. 28 - Which bacteria seem to be most frequently used in...Ch. 28 - Methylophilus methylotrophus can convert methane...Ch. 28 - Faded worn-look denim is produced with cellulase....Ch. 28 - Suppose you are culturing a microorganism that...Ch. 28 - Researchers at the CDC inoculated apple cider with...Ch. 28 - The antibiotic efrotomycin is produced by...
Additional Science Textbook Solutions
Find more solutions based on key concepts
12. Which of the following experiments could test the hypothesis that bacteria cause ulcers in humans? (Assume ...
Campbell Biology: Concepts & Connections (8th Edition)
4. Three groups of nonvascular plants are _______, ______, and _______. Three groups of seedless vascular plant...
Biology: Life on Earth
One isomer of methamphetamine is the addictive illegal drug known as crank. Another isomer is a medicine for si...
Campbell Essential Biology (7th Edition)
QUANTITATIVE Punnett Squares as Genetic Tools. The genetic characters of seed color (where Y is the allele for ...
Becker's World of the Cell (9th Edition)
What are four functions of connective tissue?
Anatomy & Physiology (6th Edition)
Police Captain Jeffers has suffered a myocardial infarction. a. Explain to his (nonmedically oriented) family w...
Human Physiology: An Integrated Approach (7th Edition)
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- 4. (a) Which of the following chemical reactions is an overall oxidation-reduction (redox) reaction that involves oxidation number changes in their reactions? A. NaOH + H₂O → Na¹ + H3O+ + OH B. HCl + H₂O → H3O+ + Cl- C. CO₂ + H₂O → H+ + CO3²- D. N₂ + 3H₂ → 2NH3arrow_forwardYou inoculate two test tubes with the same amount of identical growth medium and with the same number of identical yeast cells and grow these cells under identical conditions except for the presence or absence of oxygen. a) After 12 hours, all of the glucose in each culture has been consumed. You determine the number of total cells found in each culture and find that one culture has more cells than the other. Which culture would have the greater cell density, the one grown aerobically or the one grown anaerobically? Explain why the culture you chose above can make more cells with the same amount of glucose than the other culture.arrow_forwardAfter inoculating and incubating an agar slant from a pure broth culture of a bacterial species such as E. coli, which of the following would indicate an unsuccessful aseptic transfer? (Choose ALL that apply) a - There is fungal growth in the original broth culture tube. b- There is too much growth on the agar slant. c- There are colonies of similar morphology on the slant. d - There are red, yellow, and white colonies on the slant. e - There is no growth on the slant.arrow_forward
- You are characterizing a newly discovered organism . You have noticed that the organism grows on two carbon sources - sucrose and mannitol . Whenever you add both sugars to the medium , sucrose will be completely metabolized before mannitol . a ) What is this growth pattern called ? b ) Describe a possible growth curve of sequential metabolism of the sugars .arrow_forwardSydney Brenner isolated Salmonella typhimurium mutants that were implicated in the biosynthesis of tryptophan and would not grow on minimal medium. When these bacterial mutants were tested on minimal medium to which one of four compounds (indole glycerol phosphate, indole, anthranilic acid, and tryptophan) had been added, the growth responses shown in the following table were obtained. Mutant Minimal medium Anthranilic acid Indole glycerol phosphate Indole Tryptophan trp-1 − − − − + trp-2 − − + + + trp-3 − − − + + trp-4 − − + + + trp-6 − − − − + trp-7 − − − − + trp-8 − + − − + trp-9 − − − − + trp-10 − − − − + trp-11 − − − − + Give the order of indole glycerol phosphate, indole, anthranilic acid, and tryptophan in a biochemical pathway leading to the synthesis of tryptophan. Indicate which step in the pathway is affected by each of the mutations.arrow_forwardConsider the growth curve shown below. Cells were grown in a medium containing 1 % w/v glucose and 0.5% w/v acetate. Calculate the generation time when growing on glucose and also when growing on acetate. Pay particular attention to the split time axis with different time scales. Glucose generation time = hrs Acetate generation time = hrs 1011 1010 109 108 107 106 105 104 10 15 20 25 30 time (hours) HA log of numbers of bacteria LOarrow_forward
- Please answer the following correctly: m) Tannins are produced by many woody plants and have antimicrobial properties that can interfere with fermentation in the production of biofuels. Are tannin extracts effective in inhibiting yeast fermentation?arrow_forwardSydney Brenner isolated Salmonella typhimurium mutants that were implicated in the biosynthesis of tryptophan and would not grow on minimal medium. When these bacterial mutants were tested on minimal medium to which one of four compounds (indole glycerol phosphate, indole, anthranilic acid, and tryptophan) had been added, the growth responses shown in the following table were obtained. Mutant Minimal medium Anthranilic Indole glycerol acid Indole Tryptoph phosphate trp-1 trp-2 trp-3 trp-4 trp-6 trp-7 trp-8 trp-9 trp-10 trp-11 - Give the order of indole glycerol phosphate, indole, anthranilic acid, and tryptophan in a biochemical pathway leading to the synthesis of tryptophan. Indicate which step in the pathway is affected by each of the mutations. + 1 + 1 IIarrow_forwardImagine you work for an industrial chemical company and you have been asked to design a novel cleaning agent against a group of microorganisms. Select a specific organism representative of the group you chose, gather information about it, and identify the potential "target" within the organism suitable for your cleanser to attack, discussing how your chemical will control the growth of the organism. Consider things such as concentration, amount of time needed for exposure, or anything else that would influence efficacy.arrow_forward
- THIS IS A MULTIPART QUESTION, PLEASE ANSWER ALL Using the image: 1. How would you categorize this medium? (Living/nonliving, Synthetic/complex, Rich/minimal) Briefly explain your responses. 2. Name the ingredient(s) that match the following nutrients: a. Source(s) of carbon b. Source(s) of nitrogen c. At least one source of minerals d. At least one source of growth factors, if any 3. What about this medium would change if agar were added as an ingredient? 4. Would you expect this medium to be differential, selective, or both? Explain your response.arrow_forwardAhmad has prepared a seed culture containing recombinant Escherichia coli for the production of lipase enzyme. The seed culture was incubated for 18 hours before inoculating 5% of the seed culture into 2L of production media. a. Predict the consequences if the seed culture has been contaminated. b. Discuss whether the seed culture is meeting the criteria as inoculum or not. asap please.arrow_forwardFor each of the following situations, describe how bacteria on these growth. media would appear on the plate: A. On MacConkey agar, a bacterium that grows on the medium but is negative for lactose fermentation B. On EMB agar, a bacterium that grows on the medium but is positive for lactose fermentation C. On mannitol salt agar, a bacterium that failed to grow on the medium D. On mannitol salt agar, a bacterium that grew on the medium and fermented mannitolarrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSON
Biology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStax
Anatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:PEARSON
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Anatomy & Physiology
Biology
ISBN:9781259398629
Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:9780815344322
Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:9781260159363
Author:Martin, Terry R., Prentice-craver, Cynthia
Publisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:9781260231700
Author:Sylvia S. Mader, Michael Windelspecht
Publisher:McGraw Hill Education
cell culture and growth media for Microbiology; Author: Scientist Cindy;https://www.youtube.com/watch?v=EjnQ3peWRek;License: Standard YouTube License, CC-BY