Biochemistry
6th Edition
ISBN: 9781305577206
Author: Reginald H. Garrett, Charles M. Grisham
Publisher: Cengage Learning
expand_more
expand_more
format_list_bulleted
Concept explainers
Question
Chapter 23, Problem 4P
Interpretation Introduction
Interpretation:
How is it possible to find 14C labels on newly synthesized glucose that were originally labeled in acetates.
Introduction:
14C isotopic labeling is used to track the passage of an isotope through a reaction,
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solutionStudents have asked these similar questions
generation of one less FADH2 molecule.
Part C
B-oxidation dealls with only saturated fatty acids, but many fatty acids in natural lipids are unsaturated, meaning they contain one or more double bonds. Considering the fatty acid below, calculate the energy yield of its complete oxidation.
OH
Express your answer using three significant figures.
▸ View Available Hint(s)
ΑΣΦ
+
0
?
Submit
ATP
Tracing glucose. Glucose labeled with 14 C at C-6 is added to a solution containing the enzymes and cofactors of the oxidative phase of the pentose phosphate pathway. What is the fate of the radioactive label?
carbons?
6. Delineate the steps in the production by a human of a C20:3 45, 8, 11 fatty acid. Make sure your
scheme is metabolically plausible. For each step, list the enzymes, cofactors, and product(s).
You may start from palmitate, if that is useful. If you require an alternate starting material,
identify the material and explain why you chose that material.
Chapter 23 Solutions
Biochemistry
Ch. 23 - Prob. 1PCh. 23 - Determining the Amount of ATP Produced from Fatty...Ch. 23 - Prob. 3PCh. 23 - Examining the Labeling of Glucose from 14C.labeled...Ch. 23 - Prob. 5PCh. 23 - Prob. 6PCh. 23 - Prob. 7PCh. 23 - Prob. 8PCh. 23 - Prob. 9PCh. 23 - Prob. 10P
Ch. 23 - Prob. 11PCh. 23 - Understanding Human Energy Consumption During...Ch. 23 - Prob. 13PCh. 23 - Prob. 14PCh. 23 - Prob. 15PCh. 23 - Extending the Mechanism of Methylmalonyl-CoA...Ch. 23 - Prob. 17PCh. 23 - Prob. 18PCh. 23 - Prob. 19PCh. 23 - Understanding a Ubiquitous Series of Metabolic...Ch. 23 - Using the ActiveModel for enoyl-CoA dehydratase,...
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biochemistry and related others by exploring similar questions and additional content below.Similar questions
- Thin Layer chromatography (TLC). Explain the biochemical principle behind the separation of carbohydrates molecules by TLC as performed in practical 5. Explain which properties molecules must have to travel a short and large distance, respectively. Ketohexose sugars can form 8 different stereoisomers. How many of those isomers can be distinguished and resolved by TLC as performed in the practical and why? no more than 100 words totalarrow_forwardSources of Glucose during Starvation. The typical human adult uses about 160 g of glucose per day, 120 g of which is used by the brain. The available reserve of glucose (~20 g of circulating glucose and ~190 g of glycogen) is adequate for about one day. After the reserve has been depleted during starvation, how would the body obtain more glucose?arrow_forwarddisease. As such, a frontline treatment for Type 2 diabetes is the drug metformin, which acts indirectly to inhibit gluconeogenesis in the liver. You are a research biochemist who would like to develop new drugs that act to directly inhibit gluconeogenesis. You have just gained access to a library of thousands of small molecules of unknown activity, and you would like to identify lead compounds that have specific inhibitory activity against steps in the gluconeogenesis pathway. (a) into PEP in order to screen for inhibitors of enzymes specific to gluconeogenesis. Which enzymes do you need to purify, what cofactors and allosteric effectors do they require, and which reactants do you need to add to reconstitute the reactions for the first bypass? Which intermediates and products are generated? Your first approach is to reconstitute the initial set of bypass reactions that convert pyruvate (b) vitro reconstitution? What additional steps and enzymes are required in liver cells but are…arrow_forward
- Pernicious anemia. Purine biosynthesis is impaired by vitamin B12 B12 deficiency. Why? How might fatty acid and amino acid metabolism also be affected bya vitamin B12B12 deficiency?arrow_forwardNH3 gala. S Co A C H₂ R-C S CH₂ HN ĐÌNH ΝΗ CH₂ CH₂ NH 3. Compare and contrast how thiolase and chymotrypsin creates and stabilizes its intermediates.arrow_forward- Keto counterparts. Name the a-ketoacida-ketoacid that is formed by the transamination of each of the following amino acids: Co, a. Alanine b. Leucine c. Aspartate d. Phenylalanine e. Glutamate f. Tyrosinearrow_forward
- Glycerophospholipids Phosphatidylethanolamine 1. classify the fatty acids as essential or non-essential and also as saturated, monounsaturated, or polyunsaturated. 2. Calculate the net ATP yield per fatty acid after complete oxidation. Show your solution using a table of pathways, reducing equivalents, and ATP 3. In case the cell is in a state requiring large amount of ATP to support energy-requiring reactions/pathways, assuming that you have 1 mole of each of the said lipids are catabolized and complete oxidized, will the total net ATP yield from these two lipids be higher or lower than the sum of the net ATPs generated from each fatty acid components? Justify your answer in biochemical terms and using 5 sentences or less.arrow_forwardLong explanations are not needed. Direct answers would suffice. ***kind of in a hurry so having the answer asap would greatly be appreciated, thank you :)) a. Maltose contains 2 glucose units linked α1,4 and is a reducing sugar. i. True ii. False b. Which of the following polysaccharides contains glucose linked via β-1,4 glycosidic bonds? i. Amylopectin ii. Glycogen iii. Cellulose iv. Amylosearrow_forwardNH2 'N. NH NH NH2 Benzamidine and Leupeptin are competitive trypsin inhibitors. They are shown in their deprotonated forms at high pH. Modify the above drawings to show the protonation and charge at pH 7.0 Draw competitive inhibitors for chymotrypsin based on these structures IZ ZI ZIarrow_forward
- Properties of an Enzyme of Prostaglandin Synthesis.Prostaglandins are a class of eicosanoids, fatty acid derivatives with a variety of extremely potent actions on vertebrate tissues. They are re-sponsible for producing fever and inflammation and its associated pain. Prostaglandins are derived from the 20-carbon fatty acid arachidonic acid in a reaction catalyzed by the enzyme prostaglandin en-doperoxide synthase. This enzyme, a cyclooxygenase, uses oxygen to convert arachidonic acid to PGG2, the immediate precursor of many different prostaglandins (prostaglandin synthesis is described in Chapter 21). a)The kinetic data given below are for the reaction catalyzed by prostaglandin endoperoxide synthase. Focusing here on the first two columns, determine the Vmax and Km of the enzyme. b)Ibuprofen is an inhibitor of prostaglandin endoperoxide synthase. By inhibiting the synthesis of prostaglandins, ibuprofen reduces inflammation and pain. Using the data in the first and third columns of…arrow_forwardThe conversion of glucose-6-phosphate to fructose-6-phosphate in the glycolytic pathway (Choose all that apply) O positions a carbonyl group on carbon 2 of fructose-1-phosphate O positions an amino group NH2 on carbon 3 of fructose-1-phosphate O positions a carbonyl group on carbon 3 of fructose-1-phosphate O is an aldose-keto isomerization O positions a primary alcohol function at carbon C-1 that facilitates phosphorylation of fructose-6-phosphate O is a keto-aldose isomerization O positions an amino group NH2 on carbon 2 of fructose-1-phosphatearrow_forwardThe tortoise and the hare. Why is the regulation of phosphofructokinase by energy charge not as important in the liver as it is in muscle?arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
- BiochemistryBiochemistryISBN:9781305577206Author:Reginald H. Garrett, Charles M. GrishamPublisher:Cengage Learning
Biochemistry
Biochemistry
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Cengage Learning
Anaerobic Respiration; Author: Bozeman Science;https://www.youtube.com/watch?v=cDC29iBxb3w;License: Standard YouTube License, CC-BY