Biochemistry
6th Edition
ISBN: 9781305577206
Author: Reginald H. Garrett, Charles M. Grisham
Publisher: Cengage Learning
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Question
Chapter 20, Problem 6P
Interpretation Introduction
To propose:
The larger ratio of (ATP)/(ADP)(Pi) is calculated when the ATP synthesis occurs.
Introduction:
The enzyme of ATP synthase produces the ATP (adenosine triphosphate) which the molecule that helps in storing the energy. In all organisms this ATP is used as the energy currency of cells.
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Additional information: ATP production by the ETC and ATP Synthase per glucose varies somewhat depending on the energy required to move NADH into the mitochondria and other energy uses for the hydrogen-ion gradient. Additional questions: 1.) How many ATP's are generally yielded by oxidative phosphorylation from the catabolism of one glucose? 2.) Summarize the total ATP's obtained from a single molecule of glucose, from start to finish?
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Standard redox potentials Eo' for some common compounds in biochemistry:
Eo' (Volt)
+ 0,81
+0,42
+ 0,25
12 O₂ + 2 H+ + 2 e
NO3 + 2 H+ + 2 e
2 cyt c (ox) + 2 e
2 cyt b (ox) + 2 e
Pyruvate + 2 H+ + 2 e*
NAD + 2 H+ + 2 e
Acétoacétate + 2 H+ + 2 e
(2) acétoacétate + NADH + H+
H₂O
NO₂ + H₂O
2 cyt c (red)
2 cyt b (red)
Lactate
(3) 2 cyt c (ox) + 2 cyt b (red)
NADH + H+
B-hydroxybutyrate
+ 0,08
- 0,19
With the help of the above table, determine the spontaneous direction of the following
reactions in the standard conditions. Justify your answers by calculating the Gibbs
(free) energy of reaction in each case.
(1) pyruvate + ß-hydroxybutyrate
-0,32
- 0,35
lactate + acétoacétate
B-hydroxybutyrate + NAD+
2 cyt c (red) + 2 cyt b (ox)
Velocity, activity units/mg
protein
31
5
4
3
~
0
5
15
20
Aspartate concentration, mM
10
- Control - With CTP With ATP
25
Figure 2: Kinetics of ATCase in the presence of ATP and
CTP (based on Gerhart and Pardee, 1962).
The kinetics of the ATCase reaction were examined using increasing concentrations of aspartate, in
the presence and absence of CTP and ATP as shown in Figure 2.
a. What information can you obtain by looking at the shapes of the curves in this figure?
b. What kinetic parameter(s) change in the presence of CTP? What parameter(s) do not change?
What is the significance of these observations?
c. Answer question 2b for ATP.
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