Brock Biology of Microorganisms (14th Edition)
14th Edition
ISBN: 9780321897398
Author: Michael T. Madigan, John M. Martinko, Kelly S. Bender, Daniel H. Buckley, David A. Stahl, Thomas Brock
Publisher: PEARSON
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Chapter 18, Problem 2AQ
You wish to know whether Archaea exist in a lake water sample but are unsuccessful in culturing any. Using techniques described in this chapter, how could you determine whether Archaea exist in the sample, and if they do, what proportion of the cells in the lake sample are Archaea?
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If your instructor gave you a culture containing one species of a microorganism, how would you be able to tell whether it was
an archaean, a bacterium, a fungus, or a protist? Identify and explain characteristics you could use that would allow you to
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needed to identify each organism.
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If you visit a lake in the middle of the Amazon and take water samples to analyze under a microscope from a nearby university that has good instrumentation and there you detect microorganisms that are not visible to the naked eye. What studies would you do to determine if they are bacteria, archaea, fungi or protozoa? Explain each type of analysis and how would you rule out members of each group?
Write at least 3 paragraphs
Why would archaea that grow in extreme environments be more intensively studied than those that do not?
Chapter 18 Solutions
Brock Biology of Microorganisms (14th Edition)
Ch. 18.1 - Describe the enrichment strategy behind...Ch. 18.1 - Why is sulfate (So42) added to a Winogradsky...Ch. 18.1 - What is enrichment bias? How does dilution reduce...Ch. 18.2 - How does the agar dilution method differ from...Ch. 18.2 - How might you isolate a morphologically unique...Ch. 18.2 - What is meant by high-throughput in culturing...Ch. 18.3 - How does viability staining differ from stains...Ch. 18.3 - Prob. 2MQCh. 18.3 - Why is it incorrect to say that the GFP is a...Ch. 18.4 - What structure in the cell is the target for...
Ch. 18.4 - FISH and CARD-FISH can be used to reveal different...Ch. 18.5 - What could you conclude from PCR/DGGE analysis of...Ch. 18.5 - What surprising finding has come out of many...Ch. 18.6 - MINIQUIZ
• What is a phylochip and what can it...Ch. 18.6 - What are the advantages and disadvantages of...Ch. 18.6 - Prob. 3MQCh. 18.7 - Prob. 1MQCh. 18.7 - How do environmental genomic approaches differ...Ch. 18.7 - Prob. 3MQCh. 18.8 - Prob. 1MQCh. 18.8 - If a large pulse of organic matter entered the...Ch. 18.9 - Prob. 1MQCh. 18.9 - What is the simplest explanation for why lunar...Ch. 18.9 - What is the expected isotopic composition of...Ch. 18.10 - How could NanoSIMS be used to identify a...Ch. 18.10 - Prob. 2MQCh. 18.10 - How does MAR-FISH link microbial diversity and...Ch. 18.11 - How can stable isotope probing reveal the identity...Ch. 18.11 - What key method is required to do genomics on a...Ch. 18.11 - Prob. 3MQCh. 18 - Prob. 1RQCh. 18 - Prob. 2RQCh. 18 - Prob. 3RQCh. 18 - Prob. 4RQCh. 18 - Prob. 5RQCh. 18 - Prob. 6RQCh. 18 - Prob. 7RQCh. 18 - Prob. 8RQCh. 18 - Prob. 9RQCh. 18 - REVIEW QUESTIONS
10. Why is a microarray not...Ch. 18 - Prob. 11RQCh. 18 - Prob. 12RQCh. 18 - Q What are the major advantages of radioisotopic...Ch. 18 - Prob. 14RQCh. 18 - Prob. 15RQCh. 18 - REVIEW QUESTIONS
16. What is the advantage of...Ch. 18 - Prob. 17RQCh. 18 - Design an experiment for measuring the activity of...Ch. 18 - You wish to know whether Archaea exist in a lake...Ch. 18 - Design an experiment to solve the following...Ch. 18 - Design a SIP experiment that would allow you to...
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- Suppose you observed a bacterium growing on Mannitol Salt agar (MSA) with a yellow colony appearance in the clinical microbiology lab. Name the genus and species you are most likely observing; state its most likely oxygen needs and salt requirements; and predict if this organism would grow on MacConkey agar or Colistin Nalidixic Acid agar. If you want to control the growth of this organism in a healthcare setting, predict what disinfectants would be best to use and their modes of action. Explain your reasoningarrow_forwardThe following table depicts characteristics of five prokaryotic species (A- E). Use the information in the table to answer the following question. Trait Species A Species B None Species C R Species D Species E None Plasmid Gram Staining Variable Results Nutritional Mode Variable Negative Negative Negative Chemohete ro- Chemohete ro- Photoauto- troph Anaerobic butanolic fermentation Chemohete ro- Chemoauto- troph Aerobic methanotroph methanogen (obtains carbon and energy from methane) troph Anaerobic troph Anaerobic lactic add fermentation troph Anaerobic nitrogen fixation and aerobic photosystems I and II Thylakoids Specialized Metabolic Pathways Other Features Fimbriae Intemal Flagellum Pili membranes Which two species should have much more phospholipid, in the form of bilayers, in their cytoplasms than most other bacteria? species B and E species C and D O species A and C O species A and Barrow_forwardMake a CONCLUSION The Microorganisms that will be studied in this course includes representatives of all microscopic life forms except, the viruses. Both stained and living representatives will be examined. They exhibit diverse characteristics morphologically and nutritionally. Considerable variations can be found in their shape, cellular structure, types of motility and size. They can be autotrophic and heterotrophic; chlorophyll-free and chlorophyll containing; unicellular and multicellular; saprophytic and parasitic; and ranging from bacteria to algae, fungi and protozoa. Some concepts of diversity of morphological types among organisms can be gained by careful microscopic examination of such specimen.arrow_forward
- Ana wants to engineer a super archaea which can survive and thrive in extremely hot and hypersaline water. What characteristics should she use to achieve the organism? Provide at least 3 and justify your answer.arrow_forwardWhere would you expect to find barotolerant and piezophilic bacteria? Explain your answer.arrow_forwardA group of friends went on a hiking trip to Wadi Shab, and while swimming in the Wadi they were amazed by the variety of organisms that they saw in the Wadi and the nearby area. Adnan collected a sample of water from the Wadi, soil, and some colorful rocks because he was interested to see which organisms can be found in each sample.arrow_forward
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Archaea; Author: Bozeman Science;https://www.youtube.com/watch?v=W25nI9kpxtU;License: Standard youtube license