You performed a two-fold serial dilution, assuming that the starting concentration is 128 µg/ml (from A to C), answer the following questions: RSITY 1. What is the MIC of well A? B? C? 2. If well A is Ciprofloxacin, well B is Gentamicin and well C is Tetracycline, which is the most effective antibiotic for this sample? and why?
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- KCI for injection contains 40 mEq in 20 mL. An order requires 15 mEq, how many mL are needed?Calculate the elution volume of blue dextran (blue), myoglobin (red), and Bromocresol purple (purple) in the gel filtration chromatography below with a flow rate of 2.5 mL/min. (Take note that the black colored test tubes represent colorless eluents)Infuse heparin at 1,200 units per hour from a solution containing 40,000 units of heparin in 500 mL D5W. How many mL/hr will deliver the ordered dosage?
- A 158lb adult male diagnosed with endocarditis has an order for gentamicin 3mg/kg/day IV in 2 divided doses, in addition to vancomycin. The Gentamicin is available in 2 mL vials containing 40mg/mL. The facility protocol is to round the gentamicin dose to the nearest 10mg, then calculate the volume of solution to the nearest tenth mL. How many mL will administer per dose?From the following data, find the % (w/w) cream of tartar (KHC4H406, MM=188): Wt of sample = 1.4160 g NaOH titrant used = 20.87 mL HCI used for back titration = 1.27 mL 1.00 mL HCI 1.12 mL NaOH 1.00 mL HCI A 0.01930 g CaCO3 89.08% (w/w) 87.12% (w/w) O 47.75% (w/w) O 95.59% (W/w)A.How much of 10,000x SYBR safe would you add to 50 ml to make a final concentration of 1x? B.How will you set up the serial dilution? How many tubes do you need? What is the concentration in each? How much LB will you add to each tube? What volume of cells will you add?
- Using the formula V₁D₁ = V₂D2, what volume of a 1/100 (10-2) dilution would you need to produce 100 mL of a 1/10000 (10-4) dilution? (Note: provide your answer in decimal format to three decimal places.)Propose a serial dilution that would give total dilution of 1/158000000 (six zeros) Each tube in your dilution series must have a volume of 50 ml or less.Assume that Ali (same patient in question 1) was prescribed vancomycin 1000 mg every 36 hours. After few days, Steady-state vancomycin concentrations were obtained before and after the fourth dose, and the peak concentration (obtained ½ hour after a 1- hour infusion of vancomycin) was 34 µg/mL while the trough concentration (obtained immediately before dosage administration) was 2.5 pg/mL. Compute a revised vancomycin dose for this patient to provide a steady-state peak concentration of 48 ug/mL and a steady- state trough concentration of 17 pg/mL. a. Estimate new rate constant (Ke) and half-life (t1/2) b. Estimate volume of distribution (Vd) using this equation Cmaxss + Cminss C. Calculate the new dose interval d. Calculate the new dose
- How is 10^4 derived? Please show all calculation steps. # of cells per mL = (Average)*DF*10^4Phenylephrine and nafazolin solutions are used as decongestants to relieve nasal congestion. Your patient has been given the following prescription below. You need to prepare 100 g of this nasal spray and make it isotonic. 1. There is an available 25% w/w Phenylephrine solution in the pharmacy. How many g of this solution will you need to supply the needed 0.5% of the patient? A. 1.0 g В. 1.5 g С. 2.0 g D. 4.0 g 2. Given the amount calculated on the previous item, and if the E value of phenylephrine HCl is 0.32, calculate its corresponding sodium chloride tonicic equivalent. А. 0.16 B. 0.64 С. 1.60 D. 6.40 3. If the E value of nafazolin is 0.27, what is the sodium chloride tonicic equivalent of the patient's needed nafazolin? A. 0.0135 B. 0.1350 1.3500 D. 13.500 4. What is the combined sodium chloride tonicic equivalent of the remaining ingredients (edetate disodium (E =0.24) and sodium metabisulfite (E = 0.7)? A. 1.060 B. 0.106 С. 1.290 D. 0.129 5. To make the solution isotonic, what…For the serial dilution, your stock solution must have a concentration of 3.5 mg/mL. How much diluent must be added to the 5.3 mg/mL red cell to prepare the stock solution? Show pertinent solution/s. What are the initial concentrations used for tubes 3, 5, and 6? Show pertinent solutions. What are the dilutions of the last positive tube and first negative tube respectively? Show computation.