A series of bovine serum albumin (BSA) was prepared and 1 mL of each solution was subjected to a Bradford assay. The increase in absorbance at 595 nm relative to the concentration of protein was plotted as shown below. O Save 1.0- 0.8- 0.6- 0.4- 0.2- 0.04 0.0 0.5 1.0 1.5 2.0 2.5 BSA concentration (mg/mL) a) Using the graph, calculate the original concentration of protein present in a mixture haemoglobin and methylene blue (diluted 1:50), which gave an absorbance of 0.20 at Absorbance (O.D.)
A series of bovine serum albumin (BSA) was prepared and 1 mL of each solution was subjected to a Bradford assay. The increase in absorbance at 595 nm relative to the concentration of protein was plotted as shown below. O Save 1.0- 0.8- 0.6- 0.4- 0.2- 0.04 0.0 0.5 1.0 1.5 2.0 2.5 BSA concentration (mg/mL) a) Using the graph, calculate the original concentration of protein present in a mixture haemoglobin and methylene blue (diluted 1:50), which gave an absorbance of 0.20 at Absorbance (O.D.)
Chapter10: Reconstitution Of Powdered Drugs
Section: Chapter Questions
Problem 7.2P
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Transcribed Image Text:A series of bovine serum albumin (BSA) was prepared and 1 mL of each solution was
subjected to a Bradford assay. The increase in absorbance at 595 nm relative to the
concentration of protein was plotted as shown below.
Save
1.0-
0.8-
0.6-
0.4-
0.2-
0.04
0.0
0.5
1.0
2.0
2.5
1.5
BSA concentration (mg/mL)
a) Using the graph, calculate the original concentration of protein present in a mixture of
haemoglobin and methylene blue (diluted 1:50), which gave an absorbance of 0.20 at a
wavelength of 595 nm using the Bradford assay. Show all workings.
Absorbance (O.D.)
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