You are performing PCR for the first time using some new primers that are 25 nucleotides in length with an estimated melting temperature of 65 0C to amplify a 200 nucleotide gene. After PCR, you run an agarose gel on the samples and observe a faint band at 25 nucleotides. What is the best possible explanation for the results? The primers hybridized to multiple sites on the DNA template. Too much template DNA was added to the PCR mixture. The primers dimerized preventing DNA transcription from occurring. A nuclease was in the solution causing degradation of the DNA.

Biochemistry
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ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
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Chapter1: Biochemistry: An Evolving Science
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You are performing PCR for the first time using some new primers that are 25 nucleotides in
length with an estimated melting temperature of 65 0C to amplify a 200 nucleotide gene. After
PCR, you run an agarose gel on the samples and observe a faint band at 25 nucleotides. What is
the best possible explanation for the results?
The primers hybridized to multiple sites on the DNA template.
Too much template DNA was added to the PCR mixture.
The primers dimerized preventing DNA transcription from occurring.
A nuclease was in the solution causing degradation of the DNA.
Transcribed Image Text:You are performing PCR for the first time using some new primers that are 25 nucleotides in length with an estimated melting temperature of 65 0C to amplify a 200 nucleotide gene. After PCR, you run an agarose gel on the samples and observe a faint band at 25 nucleotides. What is the best possible explanation for the results? The primers hybridized to multiple sites on the DNA template. Too much template DNA was added to the PCR mixture. The primers dimerized preventing DNA transcription from occurring. A nuclease was in the solution causing degradation of the DNA.
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