One of your colleagues has obtained a sample of muscle phosphorylase b that is known to be relatively inactive. She has approached you for advice on how to set up an appropriate assay. She has the following items available, not all of which are appropriate for this study. Help her out by selecting the items that she should use and what their purpose is in the assay, and then explain why each of the other items would not be useful. 1. 100 UM AMP 2. 100 UM GTP 3. 100 uM glucose 4. 100 uM glucose 6-phosphate 5. Branched glycogen 6. Amylose (i.e. unbranched glycogen) 7.50 mM HEPES buffer, pH 7.5 8. 50 mM potassium phosphate buffer, pH 7.5 Write out a short explanation for each of the above items, and upload your answers by the due date.
One of your colleagues has obtained a sample of muscle phosphorylase b that is known to be relatively inactive. She has approached you for advice on how to set up an appropriate assay. She has the following items available, not all of which are appropriate for this study. Help her out by selecting the items that she should use and what their purpose is in the assay, and then explain why each of the other items would not be useful. 1. 100 UM AMP 2. 100 UM GTP 3. 100 uM glucose 4. 100 uM glucose 6-phosphate 5. Branched glycogen 6. Amylose (i.e. unbranched glycogen) 7.50 mM HEPES buffer, pH 7.5 8. 50 mM potassium phosphate buffer, pH 7.5 Write out a short explanation for each of the above items, and upload your answers by the due date.
Chapter16: Adult And Pediatric Dosages Based On Body Surface Area
Section: Chapter Questions
Problem 20SST
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Transcribed Image Text:One of your colleagues has obtained a sample of muscle phosphorylase b that is known to be
relatively inactive. She has approached you for advice on how to set up an appropriate assay. She
has the following items available, not all of which are appropriate for this study. Help her out by
selecting the items that she should use and what their purpose is in the assay, and then explain
why each of the other items would not be useful.
1. 100 UM AMP
2. 100 UM GTP
3.100 uM glucose
4. 100 uM glucose 6-phosphate
5. Branched glycogen
6. Amylose (i.e. unbranched glycogen)
7.50 mM HEPES buffer, pH 7.5
8. 50 mM potassium phosphate buffer, pH 7.5
Write out a short explanation for each of the above items, and upload your answers by the due
date.
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