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write down the following techniques of immunology and thier applications;
1.precipitation
2.agglutination
3. simple immunodiffusion
4. double immunodiffusion
5.immunoelectrophoresis
6. two dimensial and counter immnuoelectrophoresis
7.immunofluorescence
8.complement fixation
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- 1) SDS PAGE gels can be probed with NBT/BCIP to perform an immunoblot true/ false 2) the direction of migration of protiens through a matrix is due to most proteins in natur having a net negative charge true/false 3) PBST is a buffer that is used for blocking the membrane during immunoblotting. true/false 4) the buffer used during for transferring proteins to nitrocellulose methanol. true/false 7) The size of the pores of a polyacrylamide gel dcreases as concentrations of polyacrylamide increases. True or false 10) The secondary antibody used in laboratory for western blotting is conjugated to which enzyme A) Alkaline decarboxylase B) GAPDH C) Alkaline Phosphatase D) Horseeradish peroxide 13) In protien electrophroresis, what reagent present in the sample buffer is used to eliminate difference in the charge densities of protein A) SDS B) 2- mercaptoethanol C) Tris-HCL pH6.8 D) Tris- HCL PH 8.8What are the advantages/disadvantages of using a direct immunoblotting method? Compare and contrast to an indirect immunoblotting method.explain these as an Immunotherapeutic approach for cancer therapy, 1. MONOLOCAL ANTIBODIES, 2. CHECKPOINT INHIBITORS, 3. CYTOKINES 4. CANCER VACCINES, 5. ADOPTIVE CELL THERAPY ( CAR T- CELL THERAPY) EXPLAIN EACH WITH ALL DETAILS ALL DETAILS NEEDED. I WILL DISLIKE IF IT’s SHORT ANSWER. OTHERWISE I WILL RATE :)
- Why monoclonal antibodies method is better than polyclonal antibodies method in detecting specific pathogen? Explain the reasons for both selection or rejection methods..Compare and contrast Radial Immunodiffusion with it’s close relative, the Ouchterlony plate technique. How are they similar and how are they different?Can S-layer proteins be detected by immunolabelling when a capsule is present? How do you know? I need help finding the answer in the article and explain in short answer link to article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC106848/
- Name an immune immunosuppressive agent? Please provide the answer with a plagiarism-free proper explanation. Kindly also refer to the NCERT 12th Biology.Explain the complement system under the following headlines: 1. Types 2. Activation 3. Complement fixation testHow can we convince the general public and possibly even ourselves, that immunization is a necessary preventative measure for diseases that have serious complications
- Explain the following:1. What is immunologic agent drugs? 2. What is the importance of immunologic agent drugs to immunocompromised patients?Most immunizations require multiple exposures to the vaccine (i.e., boosters). Why is this the case?2. https://doi.org/10.1186/s12868-022-00692-1 (link to research) a) In the immunohistochemistry section of the materials and methods section the authors wrote “The number of positive cells in hotspot areas in ten high power fields (HPFs) in areas of demyelination and plaques in the brain stem were counted using the image analysis software (Lecia Application Suite Version 4.12.0, Welzlar, Germany).” Why were they looking at demyelination areas for this study? b) In the effect of mitoxantrone on histopathological changes in the brain section of the results section the authors wrote “Active plaques revealed inflammatory cellular infiltrates with abundant macrophages stuffed with myelin debris, an evidence of ongoing myelin breakdown.” What does macrophages stuffed with myelin debris have to do with the study?