why steroids do not show luminescence on thin layer chromatographic plates?
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why steroids do not show luminescence on thin layer chromatographic plates?
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- NMR is considered a low-sensitivity spectroscopic technique. What is the physical basis of NMR's insensitivity?Consider a bi convex lens with radius of curvatures |R1| = 30 mm and |R2| = 180 mm, ct = 5 mm and glass is N-BK7. This is used as camera lens in order to image a man who is at 1 km away from the camera. We want to resolve 10 cm on his body. Assume that λ = 550 nm. Using zemax, determine the MTF value for the resolution of R = 80 lp / mm in image plane which is placed at paraxial focus. (Hint: First, find the diameter of the lens) Which one: a.32.1 % b.28.7 % c.12.3 % d.58.5 % e.46.8 % f.57.2 % g.20.9 % h.15.6 %What is thin layer chromatography?
- What is the difference between Raman Spectroscopy and SERS ( Surface Enhanced Raman Spectroscopy).What is FRAP (Fluorescence Recovery After Photobleaching)? Draw a graph of fluorescence versus time during FRAP. What does FRAP prove?What is the difference between normal and reverse phase chromatography?
- What is Fluorescence Reflectance Imaging (RFI)? ExplainWhat is the driving force behind paper chromatography?A fluorescence recovery after photobleaching (FRAP) was performed at 37 °C. If the experiment were carried out at 10 °C, what effect would you expect on the rate of fluorescence recovery, Why?
- Why is the wavelength of the light that is released in spectrophotometric analysis longer than the wavelength of the light that is used to activate a fluorescent molecule?What are the underlying physical principles of paper chromatography? What is spectrophotometry, the essence of it?The Cavity Ring-Down Spectroscopy (CRDS) technique offers greater sensitivity compared to other conventional spectroscopy techniques. Explain the underlying mechanism of this.
