The Cavity Ring-Down Spectroscopy (CRDS) technique offers greater sensitivity compared to other conventional spectroscopy techniques. Explain the underlying mechanism of this.
Q: A solution containing two different fluorescent compounds, Ben and Jerry, were analyzed for their…
A: Beer Lamberts law states that ; A=ε CIwhere 'A' is absorbance; 'ε' is molar absorptivity…
Q: What are the processes of concentrate samples analysis in Uv-visible spectroscopy?
A: UV-visible spectroscopy is a fast and simple technique that finds the analyte concentration of a…
Q: Describe the mechanism of SERS( Surface Enhanced Raman Spectroscopy).
A: Introduction: Surface-enhanced Raman spectroscopy (SERS) is a strong microscopic observation…
Q: The following Rf values were computed from a reverse phase chromatography experiment. Arran them…
A: There are different techniques to separate a compound from a mixture of substances. The different…
Q: Explain the occurrence in which isolated peaks are formed in a characteristic x-ray spectra result.
A: X ray spectra: the spectrum that is obtained by emission of x rays which are diffracted by a crystal…
Q: How could you utilize fluorescence quenching in the different analytical application? Please answer…
A: Fluorescence quenching is a significant method for estimating restricting partiality among ligands…
Q: What are the main instrumentation components difference between Uv-visible and Fluorescence…
A: Spectroscopy :- Spectroscopy is basically an association of electromagnetic radiation with atoms ,…
Q: What do you think is/are the characteristic of sulfamethoxazole (antibiotic) that allows it to…
A: This is the structure of Sulfamethoxazole.
Q: The two most abundant pigments in the chromatography results were carotene and Xanthophyll. What…
A: A photosynthetic pigment is a pigment which is found in green plants and do not absorb all the…
Q: Why might Gamma irradiation be considered better than using UV radiation?
A: It is required to provide reason that Gamma irradiation is considered better than using UV radiation…
Q: In Gel filtration chromatography, when will you stop collecting eluents if sample is not colored?
A: Gel-filtration chromatography is a type of partition chromatography that is used to separate…
Q: Write shortly about the instrumentation of ATR spectroscopy? Answer should be to the point.
A: ATR(Attenuated Total Reflectance), is the sampling technique used in infrared spectroscopy (IR) to…
Q: phenazopyridine
A: Phenazopyridine could be a pain reliever that affects the lower a part of your tract (bladder and…
Q: What is the difference between normal and reverse phase chromatography?
A: The main Difference between Normal and reverse phase chromatography is 1) In normal phase…
Q: Explain the purpose of the Kirby-Bauer diffusion test?
A: Kirby-Bauer diffusion test/Disk diffusion method It is most widely used antibiotic susceptibility…
Q: Describe in short the process of size exclusion chromatography (molecular exclusion chromatography)
A: Size exclusion chromatography is a separation of biomolecules techniques where separation was done…
Q: What are the limitations of UV Visible spectroscopy and fluorescence spectroscopy? Include reference…
A: Spectrophotometric analysis has been widely used in biomedical science to determine the…
Q: Define iodide pump.
A: Iodide pump : An integral membrane protein that co transports Na+ and I- into the thyroid follicles.…
Q: Briefly describe the difference between chromatography and ion exchange
A: Asked : Difference between chromatography and ion exchange
Q: Briefly explain the principle behind the following techniques: (a) ion-exchange chromatography (b)…
A: Chromatography is a biophysical method for separating compounds from a mixture. It works on the…
Q: why steroids do not show luminescence on thin layer chromatographic plates?
A: Infrared absorption spectroscopy also stated as IR spectroscopy is one of the most valuable…
Q: State the principle that underlies the following biochemical tests: a) Methyl red test b)…
A: Please note that as per the guidelines of Bartleby, only question no. 1 with both subparts (a) and…
Q: What is the role of imidazole in Ni-Affinity Chromatography?
A: Chromatography is the technique where the mixture of proteins gets separated based on various…
Q: Of the two principal polymers used in column chromatography and electrophoresis, which one would be…
A: Column chromatography is a purification method that uses the polarity of compounds for the…
Q: Compare AND contrast an intercalator and an alkylating agent.
A: Intercalating agents and alkylating agents, both have effects on the cell's DNA by altering it.
Q: What is the importance of normalization of data in fluorescence spectroscopy
A: Introduction :- An electromagnetic spectroscopy technique called fluorescence spectroscopy examines…
Q: Chromatography technique works on the principle of
A: Chromatography is a technique used for separation of mixture by using a moving solvent.
Q: the principle behind this technique; affinity chromatography
A: Chromatography is a technique of separation of components of any mixture dissolved in a dynamic…
Q: Justify and explain following statements. (a) Paper chromatography is a type of partition…
A: Chromatography is a technique of separation which is used for separating the components present in a…
Q: State the principle that underlies the following biochemical tests: a) Methyl red test b)…
A: (a) Methyl Red Test Principle These bacteria initially metabolize glucose to pyruvic acid, which is…
Q: What is the importance of sllid width in fluorescence spectroscopy
A: Fluorescent microscopy is often used to image specific features of small specimens such as microbes.
Q: How does HPLC differ from ionexchange chromatography?
A: In chromatography, mixture of compounds are separated into individual components based on their…
Q: What is the working principle of raman spectroscopy.
A: Raman spectroscopy helps in determining both qualitative and quantitative information of the analyte…
Q: Column Chromatography Another name for separation in column chromatography is
A: Another name for seperation in coloumn chromatography is Adsorption column chromatography.…
Q: Different light source requires for the molecular absorption spectrometer and for an atomic…
A: A spectrometer is any instrument used to measure change in spectrum. This could be a mass-to-charge…
Q: List two situations when you would not be able to use the UV-Vis absorbance at 280 nm to determine a…
A: There are different ways of determining protein concentration. UV-Vis spectroscopy is one such…
Q: State the principle that underlies the following biochemical tests: a. Voges-Proskauer test
A: Voges and Proskauer, in 1898, first noticed the creation of a red tone after the addition of…
Q: What is doping? Explain the mechanism of mass spectrometry.
A: In semiconductor production: Doping is the intentional introduction of impurities into an intrinsic…
Q: Discuss briefly Mössbauer spectroscopy. the principle of
A: Spectroscopy is the study concerned with the measurement of spectra produced when a substance…
Q: Sulfamethoxazole is a type of sulfonamide antibiotic. Its absorbance ranges from 250-300 nm in a UV…
A: Sulfamethoxazole Sulfamethoxazole is a sulfonamide based bacteriostatic antibiotic, widely used to…
Q: Which of the following scientists use this X-ray crystallography?
A: X-ray crystallography is the experimental science determining the atomic and molecular structure of…
Q: Explain the importance of steam during the acid-fast staining procedure. Discuss the advantages of…
A: Some microbes and their structure are very difficult to be observed under the microscope . So , for…
Q: Dilute silver nitrate is sometimes applied to the eyes of newborn infants. Explain why a dilute…
A: Eye is an organ which helps us to provide vision it has several photoreceptors which receives light…
Q: What is the basic purpose of the paper chromatography?
A: Chromatography is an important analytical method used to separate a mixture of chemical substances…
Q: How is fluorescence spectroscopy beneficial for pharmacological analysis ? Please shortly answer at…
A: Fluorescence spectroscopy uses a beam of light that excites the electrons that are present in the…
Q: Explain the differences, if any, between adsorption and gas solid chromatography
A: Introduction- Chromatography is a technique that is used to separate the mixtures present in the…
The Cavity Ring-Down Spectroscopy (CRDS) technique offers greater
sensitivity compared to other conventional spectroscopy techniques. Explain
the underlying mechanism of this.
Trending now
This is a popular solution!
Step by step
Solved in 4 steps with 4 images
- Describe the mechanism of SERS( Surface Enhanced Raman Spectroscopy).What is Fluorescence Reflectance Imaging (RFI)? ExplainExplain the differences between gel electrophoresis and column chromatography. Address the principles behind each separation. Why do large molecules migrate more easily in one method and with more difficulty in the other? Which method generated the most precise results?
- Discuss the principles and applications of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry in molecular diagnostics.NMR is considered a low-sensitivity spectroscopic technique. What is the physical basis of NMR's insensitivity?Nanoparticles were added in a liquid to prompt the medication release, and it was observed that 5 mg of the medication was released in 1 liter of PBS, which accounted for 60% of the total amount of the drug. While producing an equivalent number of nanoparticles, 5 ml of 10 mg ml−1 of the drug was added. What is the efficiency of encapsulation?
- What is FRAP (Fluorescence Recovery After Photobleaching)? Draw a graph of fluorescence versus time during FRAP. What does FRAP prove?You may want to use this resource for this problem. If you do, submit the output along with your solution.You have been given a confocal microscope equipped with the following lasers, excitation filters, andemission filters:Laser Emission filter355 nm 410-470 nm405 nm 470-500 nm488 nm 500-550 nm532 nm 570-610 nm561 nm 610-650 nm640 nm 660-700 nm808 nm 720-780 nmYour task is to design an experiment to visualize the following:1. Nuclei2. A fluorescent protein in the cytosol3. A cell membrane marker antibody conjugated with a fluorophore4. Actin filaments5. LysosomesYou may choose from the following fluorophores for each of the five channels:Nuclei Fluorescent protein Membrane marker Actin marker Lysosome trackerDAPI GFP FITC AF488 Phalloidin LysoTracker RedHoechst 33342 YFP WGA-TRITC AF568 Phalloidin LysoTracker DeepRedSYTO Deep Red RFP Cy7 AF594 Phalloidin LysoTracker Blue Part 3.1Choose appropriate fluorophores for each of the subcellular structures to be imaged, taking into…Given this, if you used 6g of vitamin Z powder to make 20 ml of solution, what is the % concentration of this solution? (I gave the image since I don't know if that info is needed to solve this question.)It also gives a follow-up, if you can help here too: You work in a lab as a summer student. One of your tasks is to make sure that there is enough cell culture medium containing antibiotics to grow bacteria. One day you realize that there is only 5 ml of 10% Antibiotic stock solution in the freezer. You decide to use it all to prepare the working culture medium with 0.01% antibiotic. In the lab there is plenty of growth medium without antibiotics. (Note: dilution in medium is like dilution in water). You remember the equation to make dilutions of stock solutions. You usually use this formula to calculate the required volume of a stock solution, but you realize it can apply here as well, even though the unknown is the final volume. So, you make that dilution. Given that each bacterial…
- In UV/Visible spectrophotometer analysis for a multicomponent system, there are only two dyes used in the mixture, the two proportions should be totalled to 1.0. but on finding You got 0.6 in total. Explain the reasons for the difference.Explain the difference between a transmission electronmicrograph (TEM) and a scanning electron micrograph (SEM)Consider a bi convex lens with radius of curvatures |R1| = 30 mm and |R2| = 180 mm, ct = 5 mm and glass is N-BK7. This is used as camera lens in order to image a man who is at 1 km away from the camera. We want to resolve 10 cm on his body. Assume that λ = 550 nm. Using zemax, determine the MTF value for the resolution of R = 80 lp / mm in image plane which is placed at paraxial focus. (Hint: First, find the diameter of the lens) Which one: a.32.1 % b.28.7 % c.12.3 % d.58.5 % e.46.8 % f.57.2 % g.20.9 % h.15.6 %