Using baker's yeast it is possible to generate point mutations that destroy the kinase activity of CDK9/P-TEFB protein kinase. When genome-wide ChIP studies using anti-phosphorylated CTD antibodies and RNA-seq analysis of the mRNA purified from these cells were performed it was found that RNA Polymerase II was present in the 5' region of the coding regions of most protein coding genes, but mRNA levels were reduced for all genes tested. The cells that had this mutation were very sick and the mutation caused many of the cells to die. The abundance of mature mRNAs was lower than in non-mutant cells, and often lower than the levels of their corresponding pre-mRNA. Some of the mature mRNAs had short poly A tails and some were detected with none at all. Based on this information: Provide one reason why this mutation in CDK9 affects the levels of mature mRNAs?

Using baker's yeast it is possible to generate point mutations that destroy the kinase activity of CDK9/P-TEFB protein kinase. When genome-wide ChIP studies using anti-phosphorylated CTD antibodies and RNA-seq analysis of the mRNA purified from these cells were performed it was found that RNA Polymerase II was present in the 5' region of the coding regions of most protein coding genes, but mRNA levels were reduced for all genes tested. The cells that had this mutation were very sick and the mutation caused many of the cells to die. The abundance of mature mRNAs was lower than in non-mutant cells, and often lower than the levels of their corresponding pre-mRNA. Some of the mature mRNAs had short poly A tails and some were detected with none at all. Based on this information: Provide one reason why this mutation in CDK9 affects the levels of mature mRNAs?

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Description: Using baker's yeast it is possible to generate point mutations that destroy the kinase activity of CDK9/P-TEFB protein kinase. When genome-wide ChIP studies using anti-phosphorylated CTD antibodies and RNA-seq analysis of the mRNA purified from these

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter15: Genomes And Genomics

Section: Chapter Questions

Problem 13QP

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