Total protein: Do you think that refractive index could be used as a regular method for serum protein determinations? If yes, what limitations does it have. If not, why not? What are the major interferences in the biuret method? Refractometry method?
Q: You obtained the following raw data when setting up a Bradford standard curve: Absorbancy BSA…
A: The Bradford curve is used for estimating and analyzing the protein which is present in the given…
Q: Draw the structure before the product of glucose react further, how does oxidation occur in glucose?…
A: The open chain form of glucose gets oxidized to form the gluconic acid molecule due to the…
Q: In enzyme catalysed reactions, the energy level of the enzyme/substrate (or ES) complex is higher…
A: The enzyme is synthesized in the body and reactions are carried out in presence of enzymes. They are…
Q: If a phage is undergoing lytic growth, which protein is bound to the operator region? O Both Lambda…
A: Bacteriophages are the viruses that infect Bacteria. Bacteriophages (phages) are vital for…
Q: 26. AN ORGANIC COFACTOR IN A CONJUGATED ENZYME? A. NEITHER VITAMINS NOR MINERALS B. MINERALS C.…
A: Hi. Thank you for the question, as per the honor code, we are allowed to answer three sub-parts at a…
Q: SH HN H e. PEPTIDE ACWNEG
A: Chymotrypsin is a proteolytic enzyme that is produced by the pancreas an it is used in the small…
Q: The enzyme responsible for alpha 1,4-glycosidic linkage during glycogen synthesis is ( )…
A: Glucose is stored as glycogen in tissue. Glycogen is branched polysaccharide in which glucose…
Q: Write the name and structural formulas of the different purine and pyrimidine bases.
A: Purine and pyrimidine nucleotide synthesis is an important specialized pathway for a variety of…
Q: Discusskey impairments that are characteristic in the altered glucose metabolism of people with Type…
A: This is a condition where the body produces insulin , but the insulin response system is crippled.…
Q: During glycolysis, glucose is converted to pyruvate. Pyruvate then is converted to Acetyl coA and…
A: The process of glycolysis takes place in the cytosol while the TCA cycle occurs in the mitochondria.…
Q: Enumerate the different enzymes involved in the following lipid metabolic pathways. Show…
A: β-oxidation is the process of degradation of fatty acids to synthesize energy in the form of FADH2…
Q: Based on the principles of RP-HPLC, which sub fraction in the figure (P1 to P8) is the most polar?…
A: HPLC (High Performance Liquid Chromatography): This method is used for protein purification. Here…
Q: Differentiate water-soluble and fat-soluble vitamins?
A: Vitamins are necessary for various body functions, including vision, immunity, reproductive health,…
Q: Which statement is true regarding denaturation process? Question 21 options: Protein…
A: Introduction: Proteins are synthesized on ribosomes as linear polypeptides. As they are synthesized…
Q: This module introduced electrolytes (a substance that conducts electricity when dissolved in water).…
A: One electrolyte which is essential for maintaining the fluid balance of the body is calcium. If…
Q: Which amino acid degradation will not support gluconeogenesis? Ala Cys O Trp Leu Thr
A: The correct option will be A) Alanine Gluconeogenesis is the synthesis of glucose from non…
Q: Insulin binding to its cellular receptor causes dimerization and phosphorylation of ( )…
A: Insulin is a peptide hormone responsible many metabolic activities via insulin signalling pathway.
Q: 3. Below are the fates of the carbon skeletons of amino acids. Fill out the table with the…
A: The amino acids formed during protein catabolism may be recycled to form new proteins, converted…
Q: The following nutrient molecule is digested and transported to a cell where it undergoes further…
A: The molecule given in the question CH3-(CH2)14-COOH is hexadecanoic acid, which is commonly known as…
Q: The patient had been vomiting for several minutes, he threw up almost all his gastric content. What…
A: The present condition is observed by vomiting by the patient. The vomiting could be due to stomach…
Q: 60.A mobile, lipid soluble carrier of TWO electrons present in chloroplasts is ___. Select one: a.…
A: Photosynthesis occurs in the chloroplast. the inner membrane of the chloroplast is called the…
Q: Glutamate, produced in the first transamination reaction, reacts with oxaloacetate in the second…
A: During fasting the proteins are cleaved to amino acids some of which are partially oxidized to…
Q: what are the buffers used for biological and clinical measurements? state the properties of each…
A: A buffer is a solution that can resist change in pH upon the addition of an acidic or basic…
Q: Which description is true regarding beeswax
A: cera alba which is chemical name of beewax is a natural wax which is produced by honey bees. The…
Q: What is the purpose of flame sterilizing the inoculating loop or needle before and after using it?…
A: These questions are related to the microbiology field. Microbiology is a branch of science that…
Q: During carbon dioxide transport, a typical Bohr effect takes place at the _________ when ________…
A: Bohr effect - The effect of pH and CO2 concentration on the binding and release of oxygen by…
Q: When the acetyl-CoA produced during B-oxidation in the liver exceeds the capacity of the citric acid…
A: Acetone, acetoacetate, and beta-hydroxybutyrate are called ketone bodies. They are energy-yielding…
Q: 21. Plant pigments which can absorb light include ____. Select one: a. chlorophyll a b.…
A: A pigment is any substance that absorbs light. The color of the pigment comes from the wavelengths…
Q: 4. Tourists were cast away on a descrted island and were starving lor a week. None of them developed…
A: Since you have posted a question with multiple sub-parts, we will answer the first 3 sub-parts for…
Q: Why do some people overdose with folic acid
A: Folic acid is a type of B vitamin. It aids the body in the formation of healthy new cells. Folic…
Q: Construct an explanation for each step of DNA Isolation.
A: DNA and RNA are made up of long chains of nucleotides. Sugar molecule, ribose in RNA, and…
Q: What is the dominant biochemical activity or molecular interaction that Sickle Cell Anemia…
A: Hemoglobin is an oligomeric conjugated protein with four peptide chains joined by a…
Q: Illustrate the relationship between the following: Vitamin E, Vitamin C, Glutathione, alpha-lipoic…
A: Oxidative stress is a condition in which the concentration of reactive oxygen species (ROS),…
Q: Choose one of the strands and transcribe the strand. Show the steps (with proper label) and do a…
A: The process of gene expression involves the process of transcription followed by the process of…
Q: Kindly refer to the image and answer the question 5. Cite a metabolic pathway where active protein…
A: Protein kinases are the Enzymes that phosphorylate the Target protein by adding a phosphate group.…
Q: F2 generation of plants have purple flowers of plants have white flowers Which plants in this figure…
A: Introduction: An allele is a pair of genes (section of DNA). The pair of alleles can be either…
Q: 1. Examine the synthetic glycoprotein, Molecule A, shown below: Protein- OH OH OH OH OH ÓH OH…
A: The polymer given in the question consists of glycoprotein molecule with the different sugar…
Q: 6. The five steps involved in conversion of pyruvate to acetyl-CoA are listed below. For each step,…
A: The glucose molecules are metabolized through the glycolytic pathway to synthesize energy (ATP and…
Q: 9. Catabolism, the degradative phase of metabolism, is said to be convergent. This mea that: A. the…
A: Introduction: The term catabolism refers to the breakdown of large complex molecules to form…
Q: fill in the blanks Complete metabolism of the fatty acid stearate will produce ( )…
A: metabolism of fatty acids consists of various metabolic processes involving or closely related to…
Q: Enumerate the biological functions of DNA and RNA.
A: DNA Which is Deoxyribonucleic acid which is a polymer composed two polynucleotide chains which are…
Q: LAuden, halfan hour afler the dinner, comaining about 150g of carbohydrates 20gof f, and ag of…
A: Carbohydrates are the primary source of energy for the body. When there is energy demand in the…
Q: How would you dilute 0.1 M boric acid in 1:10 ratioto 0.01M?
A: The concentration of a substance in solution is the abundance of a constituent divided by the total…
Q: The concentration of an unknown protein sample was determined as 2.3 mg/ml. Calculate the…
A: Molarity is defined as the number of moles of a solute present per liter of a solution.
Q: Due to a nucleotide substitution, the fourth residue of H3 in a specific tissue cell of an organism…
A: Protein domains are the structural units of protein that are responsible for specific function of…
Q: 7. kynureninase with an inhibitor, 3-hydroxyhippurate, bound in the active site. The structure is…
A: Inhibitors bind to the active site of Enzyme, reversibly or irreversibly. The active site forms…
Q: A solution contains a mixture of four proteins: urease, ovalbumin, lysozyme, and alkaline…
A: Proteins are composed of a linear chain of amino acids attached together via peptide bonds. There…
Q: 2Explain the most important ceason why enzymer are necessary in iachemical feactions.
A: Enzymes are proteins which play inevitable role in the progression of biochemical reactions and…
Q: B14U A Goals n Skills Minds On Actio O Connect 2 Choose any two words in the table below, then…
A: Carbohydrates are the biomolecules that are classified as monosacharides, disaccharides and…
Q: What are the structural changes that occur in the uterus over the course of the menstrual cycle and…
A: The menstrual cycle is a sequence of natural modifications in hormone manufacturing and the systems…
Total protein:
-
Do you think that refractive index could be used as a regular method for serum protein determinations? If yes, what limitations does it have. If not, why not?
-
What are the major interferences in the biuret method? Refractometry method?
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
- What are the advantages of granulation ? during tablet formulation. DefineConsider the fluorescence spectra for bovine serum albumin (BSA) below. One solution contains phosphate buffered saline (PBS), while the other contains 1M HCL. Why does the fluorescence change the way it does? I.e., why do we observe a higher peak and a shift to the left under the presence of acid?Given that the normal range for serum protein is about 7 g/100ml and albumin is about 4 g/100ml, what clinical conclusions can you draw from the following samples A-C? If samples are not normal, what protein fraction is increased/decreased and with what disease is this protein pattern likely to be associated? Sample A: total protein = 14 g/100mlalbumin= 8 g/100 ml Sample B:total protein = 5.8 g/100ml albumin = 4 g/100 ml Sample C:total protein = 3.5 g/100mlalbumin = 2g/100 ml
- After blood collection, the red cells are separated from the serum to be used for the preparation of the stock solution. How is it done? For the serial dilution, your stock solution must have a concentration of 3.5 mg/mL. How much diluent must be added to the 5.3 mg/mL red cell to prepare the stock solution? Show pertinent solution/s. What is the appropriate diluent used for the preparation of the red cell suspension?6) 1 mL of supernatant is required for a procedure. The final colored solution proves to be too high to read accurately on the spectrophotometer. 100 μL of supernatant and 900 μL of distilled water are substituted for the original supernatant and the procedure, run as before. The reading from the standard curve is 46 mg/dL. What is the actual amount of substance in the patient serum?How is 10^4 derived? Please show all calculation steps. # of cells per mL = (Average)*DF*10^4
- K.What would happen if you reversed the electrodes on the Gel box? What would you get look like after imaging?A.How much of 10,000x SYBR safe would you add to 50 ml to make a final concentration of 1x? B.How will you set up the serial dilution? How many tubes do you need? What is the concentration in each? How much LB will you add to each tube? What volume of cells will you add?I need to prepare a standard calibration curve for gamma globulin. absorbances on Y and mg of standard protein per assay on X. used 0.1mg/ml gamma stock for tubes 2-6. (Water (ml), gamma (ml), Abosrbance)--> (.036, .004, .290) (.036, .008, .358) (.024, .016, .341) (.016, .024, .520) (.008, .032, .597) - What is the math and how do you get the standard curve?
- For the serial dilution, your stock solution must have a concentration of 3.5 mg/mL. How much diluent must be added to the 5.3 mg/mL red cell to prepare the stock solution? Show pertinent solution/s. What are the initial concentrations used for tubes 3, 5, and 6? Show pertinent solutions. What are the dilutions of the last positive tube and first negative tube respectively? Show computation.Consider the following prescription: Sol silver nitrate 0.5% 15mL Make solution isotonic for ophthalmic use. B. How many milligrams of potassium nitrate will need to be added to the prescription to make it isotonic? Report numerical value only rounded to a whole number. Need only handwritten solution only (not typed one).Table 5: Effect of Dietary Fiber on Bile Acid Adsorption Optical Density (OD) of Colored Filtrate (Reflects Amount of Bile Acid in Filtrate) 1- OD (Reflects Amount of Bile Acid Adsorbed by Food Particles) 000 Fiber- Fiber- Fiber- Fiber- Bile Acid Standard Standard Rich Rich Bile Acid Standard Standard Rich Rich With Buffer Meal Meal Meal Meal With Buffer Meal Meal Meal Meal Experimental Solution (No Trials With Bile Solution (No Fibers) Without Without With Bile Without With Bile Without With Bile Fibers) Bile Acid Bile Acid Bile Acid Bile Acid 1 1 0.55 0.4 0.00 2 1 0.61 0.4 3 1 0.52 0.32 Average 0.00 0.00 0.00 0.00 Equations (fx) Use these formulas for making your calculations. Round your answer to the nearest tenth. Averages Add the values from the three trials (or subjects); divide by three. Index reflecting the level of bile acid adsorption This index is equal to 1 - OD, where OD is optical density of the colorized supernatant of mixtures of the studied food. This index reflects…
