The predicted thermodynamic yield for anaerobic oxidation of methane coupled to sulfate reduction is -16.67 kJ/mole (under standard conditions). Which of the following can explain how ANME and SRB can make a living even when so little energy is available? High methane fluxes combined with high pressures that make the effective methane concentration higher than might be expected The ANME archaea consumes a waste product generated by the SRB which allows it to gain more energy The SRB consumes hydrogen which makes the methane oxidation more favorable for the ANME archaea The SRB also oxidizes methane, which makes the overall energy yield higher.
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- Which of these are valid sulfur cycle pathways? Sedimentation of thiosulfates Anoxic emission of sulfides Atmospheric S6 deposition O Elemental sulfur oxidation by Thiobacillus Mineralization of organic and inorganic sulfates O co2 photochemolithotrophic fixation by anaerobic bacteria Mineral formation from reduced sulfidesUnder anoxic conditions, biological denitrification of waste water by activated sludge results in the conversion of nitrate to nitrogen gas. Sludge is microorganisms, and these microorganisms carry out this reaction, this is a biological reaction. When acetic acid provides the carbon source, the reaction can be represented as follows: 5CH3COOH + 8NO3 → 4N2+ 10CO2 + 6H20 + 8HO Acetic acid Nitrate a) Is the stoichiometric equation balanced? write down the number of atoms of each species on left side and also on the right side of this equation. b) In the absence of side reactions, what is the yield of nitrogen from acetic acid in g g1? c) A certain waste water contains 6.0 mM acetic acid and 7mM NaNO3. If 25% of the acetic acid and 15% of the nitrate (NO3) are consumed in other reactions (e.g, for growth of the microorganisms in the sludge), which is the limiting substrate in the denitrification reaction? d) For the situation described in part c), what mass of gaseous nitrogen (N2) in…roblem 1An aerobic biochemical process uses a CSTR w/o recycle. The feed characteristics are asfollows: Influent substrate concentration, So = 200 mg/L; half- velocity coefficient, Ks = 50mg/L; maximum, specific substrate utilization rate, k = 5 g/g- day; yield coefficient, Y = 0.5g Xa/g substrate consumed; microorganism decay coefficient, b = 0.10 day-1.1. The process goal is the production of active biomass. Determine the hydraulicretention time that the reactor should be operated in order to maximize the reactoractive biomass concentration (Xa). How does this retention time compare with theminimum solids retention time that the system can theoretically be operated?Calculate the solids retention time safety factor.2. Based on the solids retention time for maximum Xa calculated above, estimate theactive biomass concentration (Xa) and the substrate removal efficiency (E, %)
- Some microbes like E. Coli have flexible metabolic systems that allow them to generate energy under aerobic and a erotic conditions. If E.coli is growing in the presence of O2, nitrate, and fumarate explain its metbalobic processes including which of these it will use first and whyWhat are the possible fates of methane in oxic and anoxic zones? In each case indicate what theelectron acceptor is and which type of micro-organisms are responsible for the respective reactions.Under what specific conditions would you find a thermophilic anaerobe that is a chemoautotroph? Be very specific. Also, where does it get its energy, wehre does it get itsc carbon, and specifically to which domain is it most likely to belong?
- You have evaluated the rate of aerobic degradation of organic matter in an industrial wastewater and found that it does not follow normal Monod kinetics. After some testing, you have found that the substrate degradation rate follows the following relationship instead: -rut = k0.5XaS0.5 where -rut = substrate utilization rate (mg/L•day) k = 4 (L/mg•d2) S = substrate concentration (mg/L) Xa = active microbial concentration (mg/L) You have also determined coefficients of bacterial growth on this substrate under aerobic conditions to be Y = 0.25 g VSSa/g substrate and b = 0.08/d. (VSSa, active volatile suspended solids) Estimate the effluent substrate concentration when treating the above wastewater in a CSTR (continuously stirred tank reactor) with recycle while operating at a θx (MCRT, mean cell residence time) of 4 day.The chemical reaction equation for the conversion of ethanol (C2H5OH) to acetic acid (CH3COOH) is: C2H5OH + O2 → CH3COOH + H2OIn a vinegar production industry, acetic acid is produced from ethanol during the growth of the bacterium Acetobacter aceti, whose cellular composition is CH1,8O0,5N0,2. The biomass yield from substrate is 0.14 g/g, while the product yield also from substrate is 0.92 g/g. Ammonia is used as a nitrogen source. Based on this information, answer: (a) What is the oxygen demand for acetic acid production in this crop?(b) What are the cooling requirements of a bioreactor containing 3000 L of ethanol (ρ = 789 kg/m³), to be completely converted into acetic acid?In aerobic respiration, cells generate heat as they grow. The amount of heat generated by cells is proportional to the amount of oxygen they consume. Accordingly, it was found that 460 kJ is generated per gmol of O2 consumed. Suppose that you need to choose a fermenter for optimal growth of Bacillus subtilis cells. It is known that the maximum oxygen demand of B. subtilis is 100 mol m3 h1. A vendor offers you a reactor with a cylindrical shape (2.8 m in diameter) and 15 m³ volume. The reactor includes a cooling coil with 30 m length and a pipe diameter of 6 cm, through which. For practical purposes, power input by the rotating impeller inside the bioreactor can be neglected. Does this reactor meet the cooling requirements of your production process? Note: If you think you need more information than you are provided with here to solve this problem, use online resources to find the required information. In this case, please state your source clearly. Note: You may assume that the…
- It is required to recover active extracellular enzymes from a completed fermentation mediumthat also consists of proteins (<200,000 molecular weight cut-off (MWCO)) and bacterial cells (1-2μm). A cross-flow 0.35 μm microfiltration (MF) with retentate recycled back to the fermenter willbe used over a period of 5 hours during which time flux is assumed to decline linearly. After 5 hours, assuming 95% of the bacteria are retained and given the additionalinformation below, what is the concertation of bacteria remaining in the fermenter and what area,in m2 , of MF membrane is needed? Initial bacterial concentration = 1.2 gdcw /L (dcw = dry cell weight)Volume of medium at the beginning = 15 m3Volume of medium left in the fermenter after 5 hours filtering = 1 m3Initial (starting flux) = 6.5 x 10-5 m sec -1Final flux (after 5 hours of filtering) = 3.9 x 10-5 m sec -Trace the fate of the N atom during dissimilatory nitrate reduction, nitrification, denitrification, and the anammox reaction, and indicate whether each must occur in an oxic or anoxic environmentWhat is biochemical oxygen demand (BOD), and why is itsreduction important in wastewater treatment?