The Na,K-ATPase is a(n) [Select] [Select] and K+ from [Select] that moves Na+ from
Q: If a slight deficiency in the Vitamin B1 derivative Thiamine Pyrophosphate (TPP) leads to an…
A: TPP is a cofactor used by many enzymes. TPP helps to cleave bonds near to a carbonyl carbon.
Q: Draw the structure of ethanolamine sphingomyelin formed from linoleic acid Draw the structure of…
A: Sphingomyelin is an important component of both the monolayer of phospholipids in lipoproteins and…
Q: Metabolic flux is regulated by both availability of substrates and level of enzyme activity. Match…
A: Enzymes are proteins which increase the rate of biochemical reactions. They carry out the quick…
Q: Vasopressin is a hormone that plays an important role in social behavior, sexual motivation, and…
A: Recall that: Amino acid sequences are written with N-terminal amino acid on the left and…
Q: 2. Another bodily process is the urea cycle. However, a carbamoyl phosphate is necessary to begin…
A: Carbamoyl phosphate is synthesized with the help of enzyme carbamoyl phosphate synthetase requiring…
Q: Base Adenine Guanine Cytosine Thymine Type of Bases Ring structure Number of hydrogen bonds…
A: Nucleic acid can be of 2 types: DNA (deoxyribonucleic acid) RNA (ribonucleic acid) DNA has a…
Q: 5. We're back in the lab having fun! Our current experiment calls for us to treat our cells with THC…
A: A stock or standard solution is one whose concentration is precisely known. Stock solutions can be…
Q: Why does adding acetic acid to the milk improve the extraction efficiency of the pentobarbital? You…
A: In liquid–liquid (solvent) extraction, the solute partitions itself between two immiscible phases.…
Q: Describe the four levels of protein in great depth. Desrcibe how protein structure may affect…
A: Proteins are biomolecules composed of amino acids. Amino acids are joined together through peptide…
Q: In making the experiment of protein denaturation, what usually happens upon, precipitation of strong…
A: Proteins are composed of amino acids, which are bound together by peptide linkage. Amino acids…
Q: Which of the following is an anomer of B-D-gulopyranose? O ОН I ОН т ОН I I ОН CH2OH II- Б ОН CH2OH…
A: Anomers are cyclic monosaccharides differing from each other in the configuration at carbon no -1…
Q: A gerbil is fed a normal diet including 14C-lysine. After a period of time on this diet biopsies are…
A: Gerbils are mammals , so only mammalian metabolism can be used here. In the figure below showing the…
Q: The study above was done on a wildtype enzyme and three genetically-engineered mutants. The purpose…
A: INTRODUCTION : Wild type Enzyme - These are the enzymes which are found naturally and are not…
Q: Identify the enzyme. catalyzes REDOX reactions catalyzes structural rearrangement of molecules…
A: Enzymes are biocatalysts which increase the rate of biochemical reactions. Enzymes are classified…
Q: How and where are ALT (alanine aminotransferase) and pancreatic amylase produced? What biochemical…
A: Alanine aminotransferase is an enzyme which is also called as serum glutamic pyruvic transaminase.(…
Q: ATP is a source of free energy that drives unfavorable reactions. Which of the processes are coupled…
A: The free energy changes in chemical reactions are denoted by ΔG. ∆G = ∆H − T∆S where ∆H is the…
Q: You are running an ELISA to test three patients for COVID-19.The results of the test are shown below…
A: ELISA (enzyme-linked immunosorbent assay) is a enzyme linked analytical assay in which presence of a…
Q: BIOC 384 Structure and Function of Simple Sugars Q9.1: NutraSweet (aspartame) is not a carbohydrate…
A: Carbohydrates are organic molecules that are composed of carbon, hydrogen, and oxygen. They are…
Q: Name four amino acids that can be synthesized using pyruvate as a starting material. What one(s) of…
A: Amino acids are biomolecules that have an amino group and a carboxyl group linked to the same carbon…
Q: *What are the products of saponification (hydrolysis with NaOH as the catalyst) of 1-stearoyl-2,…
A: Triglycerides are fatty acid esters of glycerols. Fatty acids are carboxylic acids with a long…
Q: What are the greatest structural features that differentiate sphingolipids from phosphoglycerides?
A: Introducion Fatty acids are the important component of lipids. Lipid is one of the important…
Q: Which of the following statements are true for enzymes? Check all that apply. The activity of some…
A: Enzymes catalyse biochemical reactions by decreasing the activation energy and increase the rate of…
Q: Draw a lipid structure. Properly label the polar and non-polar ends of the representation of a lipid…
A: Lipids are a chemically diverse group of biomolecules that have two things in common: low…
Q: Why is derivatization (i.e., transesterification) of triglycerides or lipids to fatty acid methyl…
A: Triglycerides are fatty acid esters of glycerol, where three fatty acid molecules are linked to one…
Q: Chemistry what is the correct method for protien purification if the PI of three protiens are:…
A: The proteins are composed of twenty naturally occurring amino acids that are connected via peptide…
Q: Secondary messengers and their role in the mechanisms of hormonal influence on target cells:…
A: Phosphatidylinositol (PtdIns) is a lipid molecule composed of an inositol ring and two fatty acid…
Q: 3. Compare and contrast how thiolase and chymotrypsin creates and stabilizes its intermediates.
A: Enzymes are highly specialized proteins that have extraordinary catalytic power, greater than that…
Q: Draw the electron pushing mechanism
A: Electron pushing mechanism shows the jumping of electrons in the substrate and reaction…
Q: The hormone interacts with the metabotropic receptor, as a result of further implementation of the…
A: The G protein is a protein found associated with G protein-coupled receptors. The receptor coupled…
Q: Draw the two possible Haworth structures (both alpha and beta anomers) for the following…
A: Since you have posted multiple questions with multiple sub parts, we will provide the solution only…
Q: From the three figures below, indicate which represents best the catalytic pocket of trypsin, a…
A: Serine proteases are the group of enzymes that cleaves the peptide bond in proteins, where Ser…
Q: Upon doing the experiment of Protein Denaturation, what could happen in the precipitation of strong…
A: Denaturation is the disruption and unfolding of a protein, resulting in the loss of its biological…
Q: a) Determine kcat (in units of sec-1) for a particular enzyme, given the following information: Vo =…
A: For a one-substrate enzyme-catalyzed reaction, the Michaelis-Menton equation shows the quantitative…
Q: Which of the following statements are true for enzymes? Check all that apply. The activity of some…
A: Enzymes are high molecular weight proteins (exceptions are catalytic RNAs) that catalyse biochemical…
Q: what conditions bring about acidosis and alkalosis? what are the principle metabolic function of…
A: Acidosis is a condition in which there is excess H+ ions in the arterial plasma, while alkalosis is…
Q: The purpose of DNA gyrase in replication is: to relieve positive supercoiling induced by unwinding…
A: Introduction: The process of DNA replication involves the production of an exact copy of genetic…
Q: What is the actual change in free energy at 37°C for the phosphoglycerate mutase reaction converting…
A: Phosphoglycerate Mutase: Phosphoglycerate Mutase is an enzyme involved in the glycolytic process.…
Q: Choose the best answers for each missing word from the list below. ____ regulated by ATP, Aspartate…
A: Enzymes are proteins found in the cell, these are bio catalysts that speed up the reaction without…
Q: true/ false: When proteins are denatured 1°, 2°, 3°, and 4° structure is lost.
A: Denaturation is a process in which proteins lose their original structure or confirmation. The loss…
Q: Identify the type of base and sugar group present in each nucleotide or nucleoside. HO. H₂C -CH₂ ОН…
A: A nucleotide consists of a base, a molecule of sugar and one of phosphoric acid. The sugar can be…
Q: Why is cholesterol an important steroid?
A: Steroids are the compounds that consist of three cyclohexane rings and a cyclo pentane ring in…
Q: Show a calculation of change in standard reduction potential that explains why succinate is not…
A: When two half reactions are paired in a redox reaction, the half reaction with the higher E°' will…
Q: a) Describe the three irreversible reactions of the Citric Acid Cycle. Ensure to indicate their…
A: Krebs cycle or tricarboxylic acid (TCA) cycle in aerobic organisms is the final stage of catabolism…
Q: An unknown sample was tested if there is a presence of lipid, after the test it shows that it is…
A: These are the various preliminary and qualitative tests to estimate the presence and nature of…
Q: How are intracellular and extracellular proteins degraded?
A: Proteins are composed of amino acids, which are bound together by peptide linkage. Amino acids…
Q: How does the degree of unsaturation and structure of fats affect its functionality, for example in…
A: Triglycerides or triacylglycerides or simple fats are fatty acid esters of glycerols. In…
Q: 1. Compute the concentration of the standard solutions by completing Table B.1. Report your answers…
A: Bradford assay is a method to estimate the protein concentration in the given sample. It is based on…
Q: 5. Which of the following is true about myoglobin and/or hemoglobin? O (a) The iron in Hb is in the…
A: Both hemoglobin & myoglobin are globular proteins. Our red blood cells (RBCs) are composed of…
Q: Below is a Michaelis-Menten plot for a wild-type (WT) and mutant (V105A) enzyme isolated from the…
A: The best way to find out the Vmax and Km values are by plotting the Lineweaver Burk Plot (LB Plot).…
Q: 1. Draw the structure of the polyribonucleotide UAGCCUG. 2. Draw the structure of the…
A: Nucleotides are phosphoric acid esters of nucleosides (pentose sugar containing a nitrogenous base).…
Q10
Step by step
Solved in 2 steps
- You perform a competition study on a GPCR. You have isolated the plasma membrane from cells which contains the GPCR of interest. If an agonist and an inverse agonist are at equal concentrations in your study but the inverse agonist has a 10 x higher affinity for the receptor than the agonist, what would you expect to be the overall outcome to be? More of the agonist is bound and so most of the receptor is in its active conformation and is stimulated More of the inverse agonist is bound and so most of the receptor is in its inactive conformation and is unstimulated.The top panel (a) of this figure shows the graded potential change (far right, upper, electrical trace) that results from ligand binding to the ligand gated Na+ channel. The bottom panel of this figure (b) shows a graded potential change (far right, lower, electrical trace) that results from ligand binding to a ligand gated Cl- channel. From this trace you know (Vm = -70 mV) 1. ECl- is -70 mV 2. ECl- is more negative than -70 mV (i.e., -80 mV) 3. ECl- is more positive than -70 mV (i.e., -60 mV)1 of 16 Data from an experiment is shown in the figure below. In the experiment, murine cells were treated with a specific a ligand that activates receptor R. In some cases, the cells were exposed to one of two drugs (X or Y) as well as the ligand or were left untreated (UT). After 30 minutes of treatment, the cells were lysed with a detergent-based buffer to release the soluble membrane, cyosolic and nuclear proteins. Samples from each cell extract were run (in duplicate) by SDS-PAGE (SDS-polyacrylamide gel electrophoresis) in order to separate the proteins by molecular mass (size). The separated proteins were then transferred to a nitrocellulose membrane which was then probed with different antibodies in a western blotting procedure to detect specific phosphorylated proteins or total proteins. If present in the cell extract, these proteins appear as a dark band in the relevant western blot image within the figure. Drug X Drug Y UT Ligand Ligand Ligand Western blotting antibodies…
- Cytokine receptors and tyrosine kinase receptors are similar in all of the following ways EXCEPT one. Which one is the exception? O They are both down-regulated by lysosomal degradation They both involve receptor exoplasmic domain dimerization They both result in an effector protein entering the nucleus O They both involve cytosolic domain phosphorylationWhich of the following would be expected to inhibit PKC activation by a GPCR ligand? phospholipase C inhibitor cAMP phosphodiesterase inhibitor Ras inhibitor MAP kinase inhibitorThe Graph below shows the binding curves of two proteins (A and B) for the same ligand (L). Use this Graph and determine the dissociation constant, K, for both proteins. Which protein (A or B) has a greater affinity for ligand L? Which of the two proteins would be more easily inhibited by an antagonist? 1.0 Y 0.5 2 A 4 6 B 8 [L] (μM) 10 12 14 16
- Purple foxglove is a plant containing chemicals that slow the activity of the Ca2+-Na+ antiport. What will ingestion offoxglove increase or decrease levels of Nat in the ICF?# 3 You've engineered a mutant cell where the FADD adapter was truncated. The mutant FADD only contains the Death Domain, and lacks the Death Effector Domain. What is the most likely phenotypic outcome for this mutant cell when presented with the Fas ligand? 20 E O The Fas/FasL oligomer is formed, but apoptosis is blocked O The Fas/FasL oligomer is formed, and apoptosis is hyperactivated O The Fas/FasL oligomer is not formed, and apoptosis is blocked O The Fas/FasL oligomer is not formed, but apoptosis is hyperactivated F3 $ 4 DOD 000 R F4 % 5 F5 T BARAT 6 tv @ MacBook Air F6 Y & 7 F7 U * ➤11 8 F8 · 9 F9Imagine being on the TV show Survivor of the Smartest and you are stuck on an island with only a few tools. You are given a bow, 1 arrow, and two vials. One is labeled ‘Na Pump antagonist’ and the other is labeled ‘Na Channel agonist’. The arrows are to capture food, of course. You are an average shot! What will you coat the arrow tips with and why? Please, if you are not a meat eater then pretend that you can trade the meat for a bean burrito!
- Please note whether each protein is either a Kinase (K), ATPase (A), GTPase (G), Membrane protein (M). Some proteins may need more than one letter: Dynamin [Choose ] Porin [Choose ] EGF Receptor [Choose ] CD4 [Choose ] P-glycoprotein [Choose ] Rho [Choose ] <From the Hill Plot below, the of the first binding event for the receptor-ligand system under study is: Q1 4nM Ο 10 μΜ -2 nM 2 nM Calculate the Hill Coefficient from the receptor-ligand binding data below: Q2 4 100 2 3 (0-1) 60 log 10 8 6 4 2 0 -2 -4 -6 -6 -4 -2 0 2 4 log [L] (nM) 6 8 102 of 16 Compound Binds to Elicits biological receptor response A Yes Yes B Yes No C No No Refer to the table above. Compound X is the natural ligand that binds to a receptor and stimulates a signal transduction pathway associated with the receptor. Based on the information given in the table, what are the roles of compounds A, B, and C? O A is an antagonist, B is an agonist, and C is a ligand. A is an agonist, B is an antagonist, and C has no role. A is a ligand, B has no role, and C is an antagonist. A is an agonist, B is an antagonist, and C is an agonist. A has no role, B is an agonist, and C is an antagonist.