5. We're back in the lab having fun! Our current experiment calls for us to treat our cells with THC (yeet!, delta 8 from hemp of course lol) and the final concentration of 15 µM once it has been added to the cells. We need to treat 10 mL of cells, and we don't want our treatment volume to be more than 10 μL per 1 mL of cells. What concentration should we make our stock solution?
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- Prior to subculture, Tifa used a hemocytometer to count her HepG2 cells cultured on a T-25 flask. After trypsinization, she prepared her cells by mixing 50 µL of the cell suspension with 50 μL of 0.4% trypan blue. Her observation under the microscope is as shown below: 1 (1) (ii) 3 (iii) 2 (iv) 4 Note: Count cells on the four labelled squares. Include cells touching the line on top and left. 18P 06. ¿66 Ⓡ Determine the number of viable and dead cells from her observation. What is the percentage of viability of this culture? Show your calculations in detail. Calculate the concentration of viable cells per mL in the original culture. Show your calculations in detail. Based on your understanding of cell culture, do you think she should proceed with subculture? Justify your answer.If my tissue cultures are suffering severely due to alkaloid production and accumulation in the medium. In that case, how can I overcome this problem?6) You want to determine the amount of cells in a culture. You dilute the suspension to 10-4 and plate 100ul onto an agar plate. After overnight incubation there are 30 colonies on your plate. How many cells are in your original suspension (assume your culture is 1 Liter)?
- Below are the results of our disc diffusion study using antiseptics and disinfectants. Use the results to answer the following questions: Lab Lab Lysol 1:10 diluted Disinfectant Isopropanol soap Tincture bleach of iodine ISP T of I BL DIS SO LYS Diameter in mm of zone of inhibition E.coli 40 mm 20 mm 10 mm 15 60 mm Bacillus 50 mm 35 mm 15 mm 8 70 mm cereus 201. Table 1 lists a typical recipe for growing bacteria in the lab. A researcher discovered a potentially new species of bacterium from a soil sample and attempted to grow it in this media. Unfortunately, the bacterium did not grow. Identify at least three components that you suggest adding to the medium to enable growth. Provide a reason for adding each component. Table 1. per 1000 mL 1g Bacteria culture media NazHPO4•7H2O KH2PO4 3g 5 g 1 g 0.4% (wt/vol) 0.2% (wt/vol) NaCl NHẠC1 Glucose Casamino acids2. You use tubes to test aerotolerance of bacteria. From your samples you have 3 results: A. Bacteria growing on the surface. B. Bacteria growing throughout the tube, the agar shows cracks. C. Bacteria growing about 5 mm below the surface. Please interpret each bacterial result. (Give the bacteria an oxygen classification, explain what classification means and interpret the cracks in the agar.) 3. Please explain how the use of an Eosin Methylene Blue Agar plate can help determine the type of fermenters that bacteria are. Please explain thoroughly.
- 3. One gram of soil was suspended in 9 ml saline and thoroughly mixed. From this suspension, 4 serial, 1:10 dilutions were made. 200 hundred microliters of each of the last four serial dilutions were used for spread plating on TY media, resulting in 5600 colonies, 412 colonies, 54 colonies and 8 colonies. What do you calculate as the bacterial concentration in the soil? (For your information 1 gram = 1 ml)Below are the results of our disc diffusion study using antiseptics and disinfectants. Use the results to answer the following questions: 1:10 Lab diluted Lab Tincture bleach Disinfectant Isopropanol soap Lysol of iodine BL DIS ISP SO LYS T of I E.coli 40 mm 20 mm 10mm 5mm 15mm 60 mm Bacillus 50 mm 35 mm 15 mm 8mm 20mm 70 mm cereus chemical(s) best at inhibiting both Gram- negative and Gram-positive bacteria [ Choose ] chemical least effective at inhibiting both [ Choose ] bacteriaThe presence of a capsule around bacterial cells usually indi-cates their increased disease-causing potential and resistance todisinfection. Capsules are generally viewed by:(a) Spore staining(b) Scanning electron microscopy(c) Gram staining(d) Ziehl-Neelsen staining(e) Negative staining
- Below are the results of our disc diffusion study using antiseptics and disinfectants. Use the results to answer the following questions: 1:10 Lab diluted Lab Tincture bleach Disinfectant Isopropanol soap Lysol of iodine BL DIS ISP SO LYS T of I E.coli 40 mm 20 mm 10 mm 5mm 15mm 60 mm Bacillus 50 mm 35 mm 15 mm 8mm 20mm 70 mm cereus chemical (s) best at inhibiting both Gram- negative and Gram-positive bacteria [Choose ] [Choose] chemical least effective at inhibiting both Soap bacteria 1:10 freshly diluted bleach chemical recommended by CDC for decontamination of surfaces contaminate with blod possibly carrying HIV/Hepatitis B or C viruses Tincture of lodine3. What is the concentration of a stock solution in mg/ml and µg/ml if the total concentration was 8g/L? Show your work! 4. Suppose you have a petri dish with a total bacterial count of 500,000 cells. What will be the final bacterial count if you performed the following serial dilutions? Show your work. 1:10 1:5 1:2 1:2The PD# of your culture is 165. You plated 3745 cells per well. You have recovered 12,655 cells. What's the new PD#?