The following is HPLC data. Using the standard curve determine the concentration of caffeine in each sample in µg/µl: Sample Peak area Volume of sample Amount of caffeine µg/ µl 1 1305490 1 µl 2 7836463.3 1 µl 3 3476413.7 1 µl 4 2936950.7 1 µl 5 15452894 1 µl 6 958353.7 1 µl 7 592743.3 1 µl 8 557099 1 µl 9 653410.7 1 µl 10 734443.7 1 µl
Q: A patient is to receive 25,000 units of heparin in NS 500 mL continuous infusion. The pharmacy…
A: Nurses are usually responsible for administering drugs in a variety of contexts. The nurse can…
Q: In the experiment performed for the determination of cholesterol, the function of the calibration…
A: Clinical chemistry mainly makes use of chemical processes that helps in measuring the chemical…
Q: As shown in the image below, 10 µl of a stock solution (tube #1) are serially diluted into 0.990 ml…
A: Introduction Dilution is the process of lowering the concentration of a solute in a solution by…
Q: 2-mL prefilled syringes containing 80 mg of tobramycinand 1.5-mL prefilled syringes containing 60 mg…
A: okay so it's a very easy and simple question, information we have is:- 2ml syringe=80mg of…
Q: The local anesthetic procaine hydrochloride can be administered by local injection. If the procaine…
A: Drug Medication has physiological effect and causes significant changes in the body. Factors…
Q: Which among the general tests for alkaloids can be used as a visualizing agent in detecting…
A: Alkaloids are nitrogen-containing, alkali-like naturally occurring molecules. Alkaloids are…
Q: What is measured at the A260 absorbance values?
A: DNA and RNA absorbs UV light due to heterocyclic rings of the nucleotides that is present in the…
Q: You record the absorbance of your unknown BSA sample at 0.276. What is the concentration in ug/mL of…
A: Standard curve can be used to determine the concentration of unknown sample by determining their…
Q: To estimate drug dosages, doctors use a patient’s body surface area (BSA)(in meters squared) using…
A: Body surface area (BSA) = hm60,where h is the height in centimeters and m is the mass in kilograms.…
Q: When more than one dosage strength is available, choose the most appropriate.
A: Medication administration is always followed by dosage regimen and accurate dosage calculations.…
Q: The healthcare provider orders 90 mg of a drug IVPB q6h for a child. If the label on the drug vial…
A: To dispense doses of various medications, the device uses a volume control chamber instead of a…
Q: A 4-year-old child was admitted to hospital hyperventilating, after drinking an unknown substance…
A: Question is related to nursing subject Acid base imbalance solution given below steps
Q: Calculate the quantity of glycerin needed to prepare 35-mL of the following ophthalmic solution.…
A: Dexamethasone sodium phosphate is a salt of dexamethasone that is particularly suitable for…
Q: Discuss what will happen when a chronic alcoholic person takes 3 tablets of paracetamol 500mg
A: Consumed alcohol is metabolized in the hepatocytes of liver tissue. Alcohol metabolism increases…
Q: An antacid tablet (such as Tums or Rolaids) weighs 1.3259 g. The only acid-neutralizing ingredient…
A: Antacids are over the counter medications (OTC) which are used neutralize the acid present in the…
Q: 5) Order: Potassium Chloride 80 meq p.o. daily potassium Chloride 20 meq per 15 mL Supply: 150 mL…
A: 5) Required quantity = required dose/ dose in hand * quantity in hand Required quantity = 80 meq/ 20…
Q: our patient drank the following liquids for breakfast;220 ml of orange juice,180 ml of milk and 150…
A: Nutrition is a process in which an organism uses food to support its life as food provides energy…
Q: In the experiment performed for the determination of cholesterol, the function of the calibration…
A: The calibration curve for a substance within a specified range gives the relationship between the…
Q: A patient is prescribed 0.151 g of Lidocaine intravenously. The lidocaine is available as a 12…
A: Drug formula D (Desired amount of dose)÷ H ( Dose in hand) ×…
Q: rug-B has an elimination half-life of 9 hours, an absorption half-life of 20 minutes and linear…
A: The efficacy and the plasma concentration of any drug are dependant on the elimination half-life of…
Q: Using the provided data on the activity part, what is the acquired R value? Is it acceptable or not?…
A: Absorbance is the unit measure of amount of light that passes through a particular solution at a…
Q: The amount of calcium in physiologic fluids can be determined by a complexometric titration with…
A: Titration is a common laboratory method of quantitative chemical analysis to deter mine the…
Q: Normal room temperature is about 74 degree. What is the equivalent temperature on the Celsius…
A: Temperature defines the direction of spontaneous heat flow between objects/systems. Heat flows from…
Q: What is th normal concentration of HCO3- anion in the intracellular fluid? a. 24mEq/L b. 142mEq/L…
A: INTRODUCTION Intracellular Fluid (ICF) - fluid found within the cells (cytoplasm, nucleoplasm)…
Q: Prednisone 5mg Quantity: CLXIV 5 tabs po qd x4 d, taper down by one tablet every 5 d for 15 d then…
A: Prednisone is the steroids type of drug and use as potent anti inflammatory drug
Q: A patient has the following lab. results: pCO2 = 41 mmHg; Bicarbonate = 25 mmol/L. Compute for…
A: An A.B.G or Arterial blood gas is a test which is used to measure the acidity/alkalinity & the…
Q: Table of BSA solution absorbance of concentration at 595nm. BSA Concentration (µg/ml) OD595nm Mean +…
A: Protein concentration is measured or determined using the reference standard curve. Proteins are…
Q: the prescribed order is 120mg/m2/d * 14 days PO. How many grams will the patient who has a BSA of…
A: Prescribed order - 120 mg / m2 / day. Total days for prescription - 14 days. BSA of the patient.…
Q: Calculate the protein concentration (in mg/ml) of an unknown sample with an absorbancy value of…
A: Bradford method of protein estimation is quick and simple method of protein estimation using…
Q: Why young people with anxiety attack can develop respiratory alkalosis? Which of the following…
A: We all know that the body continuously works to maintain blood's acid - base balance. This…
Q: Discuss why saline in IV bags has a 0.9% NaCl concentration.
A: Almost our body is made up of 60% of water. It is very essential for performing metabolic activity,…
Q: Given the following data set, which cuvettes could be used to make a standard curve for absorption…
A: A standard curve is made using known quantities and it can be used to estimate unknown quantities.…
Q: From the SDS for 4-nitrobenzaldehyde, what does the Hazard Code H317 refer to? O 1) Skin…
A: INTRODUCTION Hazard statement These are the phrases about hazard that is caused by chemical…
Q: Drug Clearance For example, if the ClT of penicillin is 15mL/min in a patient and penicillin has a…
A: Clearance : rate of drug elimination divided by the drug plasma concentration
Q: Count the significant digits in each of these measurements: number of significant digits ?…
A: Rules for significant digits: All non zero digits are significant digits. The zeroes between the…
Q: How many ml will you administer?
A: Paracetamol usually comes in 120mg/5ml. Ordered mg: 100mg for every 4hours/.
Q: 83 m = _____________ cm 584 µL = ________________ mL 08 g =…
A: We know, 1 m =100 cm 1 µL= 0.001 mL 1 g= 1000 mg 1 nm= 1x 10-6 mm 1 cm= 10 mm 1 mL= 0.001 L 1 mL= 1…
Q: Using the Friedewald equation, compute for the LDL-chol. of the patient.Use the mmol/L, so, if you…
A: 3 types of cholesterol makes up the Total Cholesterol (TC) content in our body. These are High…
Q: What is measured at the A280 absorbance values?
A: Proteins in solution has a absorbance maxima at 280. The aromatic amino acids are responsible for…
Q: I want to look the concentration values from these absorbance readings in order to make a graph of…
A: The Lambert-Beer law states that the light absorbed by a compound is dependent on the concentration…
Q: Urine and blood samples were taken from four different patients and analyzed with the following…
A:
Q: 400.0nm 0.01 Spectrum 1.000AT 100% PARACETAMOL -75% PARACETAMOL : 25% ALAXAN 50% PARACETAMOL: 50%…
A: The Beer-Lambert law relates the atteunation of the light to the properties of the material through…
Q: a drug that will be administered as a 500mg oral solution has the following average pharmacokinetics…
A: The plasma concentration of the drug is very important for determining the effectiveness of the drug…
Q: Dexamethasone 5 mg IM q12h for 2 doses.The label on the dexamethasone reads 8 mg/mL. How many…
A: Doctors order : Dexamethasone 5 mg IM q12h for 2 doses. Dose in hand: 8 mg/mL. We need to find out…
Q: The concentration of the synthetic opioid fentanyl should be determined in a blood plasma sample…
A: Introduction' Fentanyl is a μ-opioid receptor agonist with strong anesthetic and analgesic…
Q: A patient requires 2200 kcal/day from D50 (50% dextrose) and 8.5% aa (initial concentration). What…
A: TPN means Total Parentral Nutrition is a method of feeding in which fluids are given into a vein…
The following is HPLC data. Using the standard curve determine the concentration of caffeine in each sample in µg/µl:
Sample |
Peak area |
Volume of sample |
Amount of caffeine µg/ µl |
1 |
1305490 |
1 µl |
|
2 |
7836463.3 |
1 µl |
|
3 |
3476413.7 |
1 µl |
|
4 |
2936950.7 |
1 µl |
|
5 |
15452894 |
1 µl |
|
6 |
958353.7 |
1 µl |
|
7 |
592743.3 |
1 µl |
|
8 |
557099 |
1 µl |
|
9 |
653410.7 |
1 µl |
|
10 |
734443.7 |
1 µl |
|
Step by step
Solved in 3 steps with 2 images
- The standard curve to determine the amount of betacyanin is shown below. You extracted a red pigment from a beet disc (the mass of a disc is 2 g) using 10 ml of 20% ethanol solution. You measured absorbance of the solution above the beet disc every minute for a total time of 20 minutes. The increase in absorbance was linear during a period of time from 1 min to 10 min. The absorbance at 10 min was 0.8. Calculate the amount of betacyanin extracted from 1g of a beet tissue per minute. Explain your calculations. You can use Excel or a calculator.In the experiment performed for the determination of cholesterol, the function of the calibration curve obtained using 25-400mg/dL standard cholesterol solutions was determined as y= 0.0009x. If the absorbance of the serum sample is determined as 0.28, what is the amount of cholesterol in the sample, in mg/L?Solution Absorbance mg/ml aspirin Standard solution - 1.6 mg/mL A 0.638 0.08 mg/mL B 0.504 0.064 mg/mL C 0.376 0.048 mg/mL D 0.259 0.032 mg/mL E 0.126 0.016 mg/mL A = -log T where T = %T ÷ 100 Construct a callibration curve using the above data. Absorbance should be on the vertical axis and "mg/mL of acetylsalicylic acid" on the horizontal axis. The line should go through the origin. Using the data provided, the graph you have generated, and the procedure that was used to generate the solutions which were examined by spectroscopy, calculate the amount of acetylsalicylic acid per tablet. Commercial tablet 1 labelled as 100 mg enteric coated Absorbance = 0.16 Commercial tablet 2 labelled as 300 mg Absorbance = 0.45 Student prepared tablet from practical 5 Absorbance = 0.19 Using the data provided, the graph you have generated, and the procedure that was used…
- From the following data, find the % (w/w) cream of tartar (KHC4H406, MM=188): Wt of sample = 1.4160 g NaOH titrant used = 20.87 mL HCI used for back titration = 1.27 mL 1.00 mL HCI 1.12 mL NaOH 1.00 mL HCI A 0.01930 g CaCO3 89.08% (w/w) 87.12% (w/w) O 47.75% (w/w) O 95.59% (W/w)a) What are the methods used for cholesterol determination? Explain the basis of the method you used in your experiment by writing.b) In the experiment conducted for the determination of cholesterol, the function of the calibration curve obtained using standard cholesterol solutions of 25-400mg/dl was determined as y= 0.0009x.Since the absorbance of the serum sample was determined as 0.28, how many mg/L is the amount of cholesterol in the sampleA cholesterol screening has reported Toms blood serum contains 50mg/dal HDL and 180 mg/dal LDL and a total cholesterol of 255. Are these values in the normal range?
- Calculate the oral loading dose (LD) of digoxin for Miss Jones who weighs 60 kg. The desired plasma concentration (Cp) is 1.5 mcg/L, volume of distribution (Vd) is 7.3 L/kg, the salt factor (S) is 1 and bioavailability fraction (F) is 0.7.Give your answer to the nearest whole number of micrograms.The equation below can be used:Loading Dose = Cp desired x VdS x FCalculate the unknown concentration of the PROTEIN C with an absorbance value of A412 given the standard curve indicated in the table below. Write your final answer (NUMBER ONLY) in two decimal places rounded off. Protein concentration (µg/mL) 0 0.02 0.04 0.06 0.08 0.10 Your answer APIENT Absorbance 0.000 0.161 0.284 0.438 0.572 0.762B. You will need approximately 1 ml of the 100 µg/ml bromophenol blue (which is your most concentrated standard) to generate your standard curve. Calculate how much bromophenol blue stock solution and how much water you will combine to accomplish this. Make 1 ml of this solution using a 100-1000 μl micropipetter. Use the equation C₁V1=C2V2 μl of 1 mg/ml stock + μl of water = 1 ml of 100 µg/ml bromophenol blue
- The results observed in a patient admitted to an emergency department with confusion and oliguria were reviewed (reference ranges are given in brackets): Sodium Potassium Urea Creatinine Alkaline phosphatase Alanine aminotransferase Albumin Bilirubin Calcium 146 mmol/L 7.2 mmol/L 18.5 mmol/L 256 μmol/L 319 IU/L 32 IU/L 42 g/L 13 μmol/L 2.47 mmol/L (135-145) (3.5-5.0) (3.5-6.6) (70-150) (95-320) (a) What is the electrolyte abnormality in this patient? (b) What do the data suggest? (c) What action should you take? (5-42) (35-50) (<17) (2.12-2.62) The sample was not visibly haemolysed and was centrifuged and analysed in the labora- tory within one hour of being collected.Siturul alORK ING )A mother brings her 251b child Into the ER with q very high fevero she has already ad ministered l2 4sp of Tylenol at home. She has brought the bottle with her and the label states: Acetamaino phere syrmp 325 mg / 5mLo Administer 10-15 mg /89 every 4 hrs as needed . How many milligrams oL Tylenot has she givern? Was this asafe dose to give the child?Your 67 YO 195lb, 5ft 10in male patient diagnosed with testicular cancer has been prescribed ifosfamide 1200mg/M2/day IV for 5 days. You calculate the BSA using the mosteller method as being 2.09m2. Ifosfamide is available in 60mL vials contianing 3g of drug. a) How many mL will you administer each day?