The concentration of butanol 1.5M. Calculate the molar absorptivity ? when the solution produced an absorbance of 3.2 when using a spectrophotometer with a cuvette path length of l = 1.0 cm
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The concentration of butanol 1.5M. Calculate the molar absorptivity ? when the solution produced an absorbance of 3.2 when using a spectrophotometer with a cuvette path length of l = 1.0 cm
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- The accuracy of a spectrophotometer can be evaluated by preparing a solution of 60.06-ppm K2Cr2O7 in 0.0050 M H2SO4 and measuring its absorbance at a wavelength of 350 nm using a cell with a pathlength of 1.00 cm. The absorbance should be 0.640. What is the molar absorptivity of K2Cr2O7 at this wavelength? 3134 cm-1 M-1The measures absorbance of a solution with a pathlength of 1.00 cm is 0.544. If the concentration is 1.40 x 10^-3 M, what is the molar absorptivity for this analyte?A solution containing a mixture of the compounds X and Y had an absorbance of 0.584 at 443 nm and an absorbance of 0.501 at 520 nm when measured with a 1.00 cm cell. The molar absorptivities (E) of X and Y at each wavelength are shown in the table. What are the concentrations of X and Y in this mixture? Wavelengt h (1, nm) Molar Absorptivity (E, M-cm-1) X 443 15790 3725 520 3841 6.180x103
- What is the molar absorptivity, in M-1cm-1, for a 4.00×10-5 M solution placed in a 2.00 cm cuvette that is measured to have an absorbance of 0.263?What is the molar absorptivity of a 1.0 x10-5 M analyte with an absorbance reading of 0.003 at 517 nm wavelength?Calculate the molar absorptivity of benzene (FW = 78.11 g/mol) at 256 nm if a 25.80 mg of benzene per 250.0 mL solution using hexane (FW = 86.18 g/mol) as the solvent exhibited an absorbance of 0.2660 in a 10.00 cm cuvette. Pure hexane has negligible absorbance at this wavelength.
- A student researcher performed a chromatographic separation of caffeine and aspartame. The retention time for caffeine, te, was found to be 200.8 s with a baseline peak width, we, of 16.1 s. The retention time for aspartame, ta, was 258.7 s with a baseline peak width, wa, of 20.6 s. The retention time for the unretained solvent methanol was 44.2 s. Calculate the average plate height, H, in micrometers for this separation, given that it was performed on a 22.1 cm long column. H = Calculate the resolution, R, for this separation using the widths of the peaks. R = 88.2 R₂5N = 3.16 Calculate the resolution if the number of theoretical plates were to increase by a factor of 2.5. 3.533 Incorrect μmA spectrophotometric method for the quantitative determination of the concentration ofPb2+ in blood yields an Ssamp of 0.133 for a 1 mL sample of blood that has been diluted to 6 mL. A second sample is spiked with 1 µL of a 1467 ppb Pb2+ standard and diluted to 6 mL, yielding an Sspike of 0.491. Determine the concentration of Pb2+ in the original sample of blood.An unknown chemical has an absorbance of 0.265 in a 1.0cm cuvette at a concentration of 0.703 M. What is the chemical's absorptivity constant in M cm? Report your answer to three decimals.
- SPECTROPHOTOMETRY 1.) Slope of regression line or Molar absorptivity (µM -1cm-1) is 0.0253 and absorbance of unknown solution is 0.131. What is the concentration of the unknown solution (µM )?The nitrite in a series of standard solutions (mg/L, n = 5) are converted to azo dye and the slope of the calibration curve is 2.0 ppm1. A 10.00-mL mineral water sample is treated in the same way as standards and diluted to a final volume of 100.00-mL. It gives an absorbance of 0.80. The absorbance of blank solution under the same conditions is 0.10. Calculate ppm (mg/L) of NO2 (46 g/mol) and the molarity of NANO2 (69 g/mol) in the original sampleA toluene solution (1.50 mg / L) shows an absorbance of 0.30 in a cuvette with a 0.025 mm optical path. Calculate the limit of detection (in ppb) for toluene if the noise with the solvent spectrum is 0.002 absorbance units in a 0.050 mm cuvette.