The concentration of a glucose solution was determined via a DNS assay, and it was determined that a 150.0 mM solution would have an absorbance of 0.156. If the pathlength used was 0.95 cm, what is its molar absorptivity constant, in L cm mol 1?
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- A student prepared four standard solutions of (NH4)2Fe(SO4)2 and measured their absorbance at 505 nm. She created a calibration plot with the best-fit line of y = 9372 x + 0.0539. She measured the absorbance of her diluted unknown sample solution as (4.69x10^-1) nm. If she prepared her diluted sample solution by diluting 10.00 mL of the original solution to a total volume of 50.00 mL with deionized water, what is the concentration of Fe2+ in her original solution? Answer in units of M. Note: Your answer is assumed to be reduced to the highest power possible.The standard curve was made by spectrophotographic analysis of equilibrated iron(III) thiocyanate solutions of known concentration. You are asked to analyze a Fe(SCN)2+ solution with an unknown concentration and an absorbance value of 0.392. The slope-intercept form of the equation of the line is y = 4538.1x +0.0077. The unknown was analyzed on the same instrument as the standard curve solutions at the same temperature. What is the Fe³+ concentration of the unknown solution? [Fe³+] = mol/L Absorbance Iron(III) thiocynate standard curve 1.0 V 0.5 0.0001 Fe³+ concentration (M) 0 0.0002Blue Blue dye stock solution 0.293 M Absorbance at 630 nm 0.00265 Calibration curve y = 0.0833x A solution is prepared by diluting 2.81 mL of the blue dye stock solution to 25.00 mL. The measure absorbance for the prepared solution is listed in the data table. (a) What is the theoretical molar concentration? [Blue]theoretical | × 10 |M (b) What is the experimental molar concentration? [Bluelexperimental x 10 M (c) What is the percent error? Percent error (blue) =
- 3.) The accuracy of a spectrophotometer is evaluated by preparing a solution of 60.06 ppm K2Cr2O7 and measuring its absorbance at a wavelength of 350 nm in a cell with a pathlength of 1.00 cm. The expected absorbance is 0.640. What is the expected molar absorptivity of K2Cr2O7 at this wavelength?The molar absorptivity constant of a particular chemical is 3.9 dm3 mol–1 cm–1 . What is the concentration (in Molar) of a solution made from this chemical that has an absorbance of 0.410 with a cell path length of 1.0cm?A spectrophotometric method for the quantitative analysis of Pb2+ in blood has a normal calibration curve for which Sstd=(0.296 ppb−1)×Cstd+0.003. What is the concentration of Pb2+ in a sample of blood if Ssamp is 0.397?
- What is the absorbance of a 2.33×10−4M2.33×10−4M solution of a compound with a molar absorptivity of 1.05×103M−1cm−11.05×103M−1cm−1 in a 1.00 cm cell?Caffeine, C8H10O2N4 H2O (MW = 212.2 g/mol) has been shown to have an average absorbance of 0.644 for a concentration of 1.783 mg per 100 mL at 272 nm. A sample of 3.658 g of a soluble coffee product was mixed with water to a volume of 500 mL and a 25 mL aliquot was transferred to a flask containing 25 mL of 0.1 M H2SO4. This was subjected to the prescribed clarification treatment and made up to 500 mL. A portion of this treated solution showed an absorbance of 0.666 at 272 nm. Assumbe b = 1.0 cm. Calculate the % caffeine (w/w) in the sample.Suppose that a solution has an absorbance of 0.250 at a wavelength of 450 nm. If the concentration of the solution is 22 μM, what is the value of the molar absorptivity? The data were taken with a standard 1-cm cuvette. molar absorptivity in (L cm^−1 mol^-1):
- The absorbance of an unknown dye solution is measured to be 0.47 by a spectrometer. The linear equation of the spectrometer's calibration plot is y= 2156.5 x +0.0213, where y represents absorbance and x represents concentrations of dye. What is the molar concentration of the unknown sample (in mole L)? O2.08e4 O 2.08e-4 0 125e3 0 125e-3(5) A sample of sodium phosphate is dissolved in water to give 100.00 mL of solution (Solution A). Then this solution is diluted 5 times to give the final solution (Solution B). Solution B is Known to contain 3.920 x 1022 number of PO43- ions, which has an absorption peak at 460 nm and absorbance value of 0.355 in a 1.000 cm cuvette. (i) Calculate the molar absorptivity of Solution B. (ii) What mass of sodium phosphate (MM 163.94 g/mol) dissolved in Solution A.A molecular exclusion column has a diameter of 7.8 mm and a length of 30 cm. The solid portion of the particles occupies 20% of the volume, the pores occupy 40%, and the volume between particles occupies 40%. (a) At what volume would totally excluded molecules be expected to emerge? (b) At what volume would the smallest molecules be expected? (c) A mixture of polymers of various molecular masses is eluted between 23 and 27 mL. What does this imply about the retention mechanism for these solutes on the column?