Questions 11-14. Melia and Sophie performed a PCR experiment using DNA purified from their phage Zucchinibread. They did a positive control, a negative control, and an experiment using subcluster B1 primers. They ran a 0.8% gel (shown below). 10KB 3Kb IKb 0.5Kb IKB DNA Ladder Negative Control Positive Control Phage DNA+ B1 Primers

Biology 2e
2nd Edition
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark
Chapter17: Biotechnology And Genomics
Section: Chapter Questions
Problem 1VCQ: Figure 17.7 You are working in a molecular biology lab and, unbeknownst to you, your lab partner...
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Questions 11-14. Melia and Sophie performed a PCR experiment using DNA purified from their phage Zucchinibread. They did a positive control, a negative control, and an experiment using subcluster B1 primers. They ran a 0.8% gel (shown below).
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Negative Control
Positive Control
Phage DNA+ B1 Primers
Transcribed Image Text:Questions 11-14. Melia and Sophie performed a PCR experiment using DNA purified from their phage Zucchinibread. They did a positive control, a negative control, and an experiment using subcluster B1 primers. They ran a 0.8% gel (shown below). 10кb 3Kb 1Kb 0.5Kb -A IKB DNA Ladder Negative Control Positive Control Phage DNA+ B1 Primers
Melia and Sophie added DNA from a C cluster phage and C cluster primers to PCR mix for their positive control. Does this control look as you would expect on the gel? Yes, or no? Explain your answer.
Transcribed Image Text:Melia and Sophie added DNA from a C cluster phage and C cluster primers to PCR mix for their positive control. Does this control look as you would expect on the gel? Yes, or no? Explain your answer.
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