Prepare one dose of 0.6 mg kanamycin in 2mL diluted from a stock solution of 20 mg/ml. How much kanamycin and how much diluent are used?
Q: A fusion protein can be synthesized to carry a specific tag, which is useful in identifying the…
A: Amino acids are the biomolecules that contain amino and carboxyl groups. Proteins are the polymer of…
Q: Chitin is a storage form of fuel in plant cells is the storage form of glucose in animals provides…
A: Biomolecules are molecules found in living organisms that play a role in their chemistry. They are…
Q: This graph represents a standard curve from a BCA assay for protein concentration. If your BCA…
A: Standard curve can be constructed by using the known concentrations of a sample and their…
Q: individuals with cystic fibrosis produce thick mucus in the lungs, resulting in a higher-than-normal…
A: At a biochemical level, cystic fibrosis is a disease caused by a mutation in the CFTR gene. Gene…
Q: 10. Assume you have the following stock solutions: 10% sodium dodecyl sulphate (SDS) V'S M NaOH…
A: Dilutions of any solutions can be calculated using the formula: C1V1 = C2V2 Where, C1= concentration…
Q: For the peptide structure in #3, (ASP-ALA-THR-LYS-GLY), A. Determine the net charge of the…
A: pKa is the pH at which a weak acid is 50% dissociated. If the pH is less than pKa, the ionising…
Q: Shown below is the structure of Drug 1, that binds to the E. coli ribosome. Indicate whether each…
A: Since, you have asked multiple questions from multiple concepts, we have answered all 4 subparts of…
Q: Draw the resonance structure of a tripeptide peptide backbone, indicating the N- and C-terminal and…
A: Peptides are molecules composed of amino acids joined together through peptide bonds. The peptide…
Q: cetyl CoA is carboxylated to malonyl CoA during fatty acid biosynthesis: a) for thermodynamic…
A: Acetyl-CoA carboxylases enzyme catalyzes the acetyl-CoA carboxylation and produced malonyl-CoA.…
Q: 13. Fill in the blanks using the keywords. +2 NADH +NAD+2 2 coenzyme A MITOCHONDRIA + CO₂ Glycolysis…
A: Introduction Glycolysis is a first step of cellular respiration. Glycolysis consists of 10 steps. In…
Q: 3. A student in my lab was trying to express the cytosolic (not membrane bound) enzyme PseG and…
A: The expression of proteins in a prokaryotic or eukaryotic cell involves transcription and…
Q: What is the advantage of having an amino acid with a negative charge? Please explain using an…
A: Amino acids - are building blocks of proteins and alpha carbon of amino acids contains amine group,…
Q: Given the amino acid chain below, get the (a) translation strand, (b) transcription strand strand,…
A: Amino acids - are building blocks of proteins and alpha carbon of amino acids contains amine group,…
Q: Assume that the experiments performed in the absence of inhibitors were conducted by adding 5 μL of…
A: We are asked to find some enzyme kinetics parameter that has a unit of s-1 . And in order to find…
Q: Discuss how the cell uses RNA molecules to synthesize proteins.
A: The majority of living things and viruses include the molecule known as ribonucleic acid (RNA). It…
Q: 6. A portion of a gene is shown below. 5'-ATGATTCGCCTCGGGGCTCCCCAGTCGCTGGTGCTGCTGACGCTGCTCGTCG-3'…
A: mRNA, or messenger RNA, is a type of RNA molecule that plays a central role in the process of…
Q: Formation of cholesterol "fund" in the liver.
A: Cholesterol contains 27 carbon atoms all supplied by acetyl CoA. Cholesterol has 3 six-membered…
Q: A 24-residue peptide called Pandinin 2, isolated from scorpion venom, was found to have both…
A: Proteins are composed of amino acids, which are bound together by peptide linkage. Amino acids…
Q: NADPH inhibits glucose-6-phosphate dehydrogenase. What kind of inhibitor is NADPH?
A: Glucose-6-phosphate dehydrogenase (G6PD) is found in the cells of many organisms. It plays an…
Q: Which one of the following statements about coenzyme Q is not true? a. coenzyme Q is lipid-soluble…
A: Coenzyme Q or ubiquinone or CoQ is an electron carrier that accepts electrons from NADH and FADH2,…
Q: 3. A. Briefly discuss the four levels of structure in proteins. Knowing that the 3-dimensional shape…
A: Protein is a polypeptide made up of amino acids. The sequence of amino acids determines the…
Q: The four drugs A, B, C and D have the same IC50 values. Which of them has the highest efficacy? 100…
A: Drugs are substances that can alter the body and mind, and are often used illegally to create a…
Q: The gene encoding the E. coli enzyme enolase begins with the sequence ATGTCCAAAATCGTA. What is the…
A: The flow of genetic information from DNA to RNA and then RNA to protein is called central dogma. It…
Q: Write the line structure of each of the following peptide at pH7 and identify how many peptide bond…
A: To calculate the number of peptide bonds in a peptide, you need to know the number of amino acids it…
Q: Prepare a schematic diagram and present it as though it were a Figure in a publication (scientific…
A: Binding of ligand to receptor or protein can be specific and non-specific. For the genuity of the…
Q: What makes RNA a ribozyme? Why are DNA enzymes absent?
A: Enzymes are biomolecules which can catalyze the biochemical reactions. These can be proteins, RNA or…
Q: Lysozyme is an enzyme that hydrolyzes bacterial cell wall…
A: Lysozymes are antibacterial agents that have the ability to hydrolyze the cell wall of bacteria. The…
Q: 8. The amino acid sequence for the beginning of the globular protein myoglobin is:…
A: Proteins are composed of amino acids, which are bound together by peptide linkage. Amino acids…
Q: Depending on the type of microbe, the pyruvate generated in glycolysis can be used for fermentation…
A: Glycolysis is the process of breakdown one molecule of glucose into two molecules of pyruvate and…
Q: 2. Here is data to plot another STANDARD CURVE... Again, carefully consider how to scale and label…
A: Polyacrylamide gel electrophoresis (PAGE) is a technique used to separate proteins based on their…
Q: OH HO "OH Enzyme A 0= отва OH OH HO OH
A: Glycolysis is a metabolic pathway that converts glucose into pyruvate. The site of this metabolism…
Q: Name of Enzyme Class Number of Enzyme Reaction Catalysed Hexokinase Phosphorylates glucose…
A: Glycolysis is a pathway that converts glucose molecules into energy by breaking into pyruvate…
Q: Genetic disorders of urea synthesis
A: As a result of our metabolism, amino-acid metabolism specifically, a lot of ammonia is produced in…
Q: 6. A. List all of the ionizable functional groups that are found in insulin when in aqueous…
A: Isoelectric Point The isoelectric point (also known as the isoelectric point, pI, or IE) is the pH…
Q: Draw the integrated metabolic pathway during fed state. Include at least three metabolic pathways…
A: The integrated pathway of metabolism refers to the interconnected series of chemical reactions that…
Q: Two common methods of denaturing proteins in the lab are to increase the temperature and/or add…
A: Proteins are biomolecules with diverse biological roles. The function of a protein depends on its…
Q: 2. In one paragraph, refute or support the statement: "Photosynthesis and oxidative phosphorylation…
A: There are a lot of metabolic processes that essential serve the opposite purposes of each other.…
Q: 2. A. Draw the chemical structure of the tripeptide CYS-ALA-SER at pH 9.0. Note: thiols also ionize…
A: Proteins are composed of amino acids, which are bound together by peptide linkage. Amino acids…
Q: Can you explain the process of beta-oxidation for saturated and unsaturated fatty acids, and the…
A: Since you have posted multiple questions, we will provide the solution only to the first question as…
Q: d. Much of the experimental research on the propagation of nerve signals has focused on an…
A: Nernst potential (V) can be calculated using following equation V = RT/zF ln (Xout/Xin) where V is…
Q: The following prokaryotic DNA is transcribed into RNA and the mRNA transcript is translated into…
A: The region of prokaryotic DNA that will be transcribed into mRNA is determined by the presence of a…
Q: Explain this krebs cycle
A: Cellular respiration is a collection of three metabolic pathways that generate ATP by the oxidation…
Q: 1.a)Which one of the following vitamins is fat-soluble? Vitamin B12 Vitamin D Vitamin B1 Vitamin B3…
A: Introduction Vitamins are the organic substances which is required for our growth and development.…
Q: 1) Why is the GPCR referred to as a GEF? What would occur in G protein signalling if the Ga subunit…
A: This answer explains the concept of G protein-coupled receptors (GPCRs) and their role as guanine…
Q: Draw a UV-Vis spectrum for each protein (shown in the table below) (A, B, C, and D) from 240 – 480…
A: UV-Vis spectroscopy is a technique that measures the absorption or transmission of ultraviolet and…
Q: Liver NADH increases gluconeogenesis. How do you interpret this?
A: NADH is an important molecule involved in cellular respiration and energy metabolism. It is a…
Q: A polar functional group that forms disulfide bridges and stabilizes protein tertiary structure is A…
A: The sequence of amino acids in the polypeptide chain is referred to as the fundamental structure of…
Q: How do you draw different amino acids at ph 7? can you show me an example which I can follow to draw…
A: Amino acids are the building blocks of proteins. They are organic compounds that contain a carboxyl…
Q: In the traditional alkaline lysis method, what is the purpose of Solution 1? For what is its use?
A: In the traditional alkaline lysis method for plasmid DNA isolation, Solution 1 (also called…
Q: From this discussion of the hormonal regulation of glycogen metabolism. Read this material and…
A: Glycogen metabolism is the process by which glycogen, a branched polysaccharide, is synthesized and…
Prepare one dose of 0.6 mg kanamycin in 2mL diluted from a stock solution of 20 mg/ml. How much kanamycin and how much diluent are used?
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
- Streptomycin sulfate 400 mg IM daily is ordered for a child weighing 77lbs. The recommended dosage is 20-40 mg/kg/day once daily. A 1 gram vial of streptomycin sulfate is available in powdered form with the following instructions: Dilution with 1.8 mL of sterile water will yield 400mg per mL. If safe, how many mLs will you give?Consider the following from the Ampicillin for Injection, USP package insert: For Intramuscular Use: Label Claim 250mg 500mg 1g 2g Amount of Diluent (mL) Approx. Volume (ml) 0.9 1.7 3.4 6.8 1 2 4 8 a) What is the amount of diluent needed to reconstitute a 1 g vial? b) What is the powder volume for a 1 g vial of ampicillin? Approx. Concentration (mg/mL) 250 250 250 250 Later in the package insert you see: For Direct Intravenous Use: Add 5 mL Sterile Water for Injection or Bacteriostatic Water for Injection to the 250 and 500 mg vials and administer slowly over a 3 to 5-minute period. Ampicillin for Injection, 1 g or 2 g, may also be given by direct intravenous administration. Dissolve in 7.4 or 14.8 mL Sterile Water for Injection or Bacteriostatic Water for Injection, respectively, and administer slowly over at least 10 to 15 minutes. c) What is the amount of diluent needed to reconstitute a 1 g vial of ampicillin for direct IV injection d) What is the total amount of volume that will…If a powder in a 2gm vial diluted with 6.8ml of diluent provides 8ml of solution and each ml contains 250mg of a drug, how many ml is needed to give 750mg.
- A 5% dextrose in 1/2 normal saline (D5 1/2 NS) solution is commonly administered to patients needing post-operative IV fluids. How could you prepare 500 mL of this solution? Dextrose is 5% (m/v) and the NaCl is 0.45% (m/v).A pediatric patient is prescribed 40 mg of prednisolone per day in two divided doses. How many divided doses are available in 6 fluid oz. of oral solution containing prednisolone 20mg/5ml dispensed to this patient?A vial of Doxorubicin reads 0•5g per vial. Instructions say to reconstitute each 12mg with 2•5ml of NS. How many ml of NS will be needed to reconstitute the vial of the recommended concentration? please show working
- A prescription order calls for 1.25 g of vancomycin in 500 mL of NS (0.9%/100mL). After reconstitution vancomycin injection is available in a strength of 50 mg/mL. How many mL of vancomycin injection should be drawn up to prepare this prescription?How many mL of paracetamol solution is needed for a day if 37.5 mg dose every 6 hours is needed from 10mg/mL solution? Choose the best answer.