The SARS-Cov-2 virus is the virus that cause COVID-19. The viral protease is a known target for finding potential viral inhibitor. The activity of the viral protease can be assayed using the synthetic peptide substrate attached to 7-amino-4-carbamoylmethylcoumarin (PACC). The viral protease cleaved the substrate producing the fluorogenic ACC which can be monitored using a spectrofluorometer. The following is data from SARS-Cov-2 protease (0.1 mg of enzyme) upon reaction with various concentrations of the substrate PACC.
Plot the appropriate graphs to find:
- The approximate Vmax from the Michaelis-Menten curve.
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From the graphs given, can you find the Km from the Michaelis-Menten curve and the Lineweaver-Burke plot.
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