Just considering stripe 3, draw the expected pattern of expression for eve stripe 3 in a knirps mupw m mutant. Figure 2 Regulation of even-skipped by maternal and gap gene gradients. 1 2 3 4 5 6 7 anterior posterior 2 enc Bcd Gt- 2 F Kr 3+7 enc 4+6 enc C Hb E Hb 3+4 Kni 6 B.
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- Contrast the role of the MCM1 protein in different yeastcell types shown in Figure 12-10. How are the a-specificgenes controlled differently in different cell types?The Wnt planar polarity signaling pathway nor-mally ensures that each wing cell in Drosophila has a sin-gle hair. Overexpression of the Frizzled gene from a heat-shock promoter (hs-Fz) causes multiple hairs to grow frommany cells (Figure Q15–5A). This phenotype is suppressedif hs-Fz is combined with a heterozygous deletion (DshΔ)of the Dishevelled gene (Figure Q15–5B). Do these resultsallow you to order the action of Frizzled and Dishevelledin the signaling pathway? If so, what is the order? Explainyour reasoning23 of 41 Which of the following proteins is NOT encoded by a proto-onoogene? O Myc O Ras O E-cadherin Src O EGF receptor
- draw the p21 promoter in a cell with E2FIn Fig. 13.18, gene 2 seems to be expressed at higher levels than gene 5. Justify the claim that the two genes have similar profiles and might be coregulated. Mutations in an autosomal gene in humans cause aform of hemophilia called von Willebrand disease(vWD). This gene specifies a blood plasma proteincleverly called von Willebrand factor (vWF). vWFstabilizes factor VIII, a blood plasma protein specified by the wild-type hemophilia A gene. Factor VIIIis needed to form blood clots. Thus, factor VIII is rapidly destroyed in the absence of vWF.Which of the following might successfully be employed in the treatment of bleeding episodes in hemophiliac patients? Would the treatments workimmediately or only after some delay needed forprotein synthesis? Would the treatments have only ashort-term or a prolonged effect? Assume that allmutations are null (that is, the mutations result in thecomplete absence of the protein encoded by the gene)and that the plasma is cell-free.a. transfusion of plasma from normal blood into avWD patientb. transfusion of plasma from a vWD patient into adifferent vWD patientc. transfusion of plasma from a hemophilia A…
- Given the following Western blot results showing levels of proteins over time in three yeast cell extracts after the induction of high level expression of the CDH1 protein. WT GAL-CDH1 GAL-CDH1, cdc23-1 0 30 60 120 0 30 60 120 0 30 60 120 Time (min) - Pds1 - Clb2 - Ase1 - Kar2 Note: The GAL-CDH1 indicates that the yeast strain contains a transgenic cdh1 gene under the control of the GAL promoter. This means that the researcher can control expression of that cdh1 gene. In these three experiments (three yeast strains), time 0 is the point at which GAL- controlled genes begin to get expressed (WT lacks transgenes). In this experiment, four proteins are being probed for in three different yeast strains (1. wild-type, 2. a strain containing the cdh1 gene under GAL control (see note), and 3. the GAL-CDH1 strain that is also harboring a cdc23 loss of function allele (allele 1), which is a component of the A The upper (larger) band in the Ase1 lanes is non-specific background band; disregard…Given the following Western blot results showing levels of proteins over time in three yeast cell extracts after the induction of high level expression of the CDH1 protein. WT GAL-CDH1 GAL-CDH1, cdc23-1 0 30 60 120 0 30 60 120 0 30 60 120 Time (min) | Pds1 - Clb2 - Ase1 - Kar2 Note: The GAL-CDH1 indicates that the yeast strain contains a transgenic cdh1 gene under the control of the GAL promoter. This means that the researcher can control expression of that cdh1 gene. In these three experiments (three yeast strains), time O is the point at which GAL- controlled genes begin to get expressed (WT lacks transgenes). which is a component of the A In this experiment, four proteins are being probed for in three different yeast strains (1. wild-type, 2. a strain containing the cdh1 gene under GAL control (see note), and 3. the GAL-CDH1 strain that is also harboring a cdc23 loss of function allele (allele The upper (larger) band in the Ase1 lanes is non-specific background band; disregard it.…© Macmillan Learning The coronavirus pandemic that first emerged in 2019 resulted in a large number of people contracting the COVID-19 disease caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Although a large percentage of those infected experienced only mild symptoms, a significant percentage of individuals without complicating health conditions fell seriously ill or died. Suppose researchers hypothesize that a phenomenon called "cytokine storm" may contribute to having a more severe illness from COVID-19 disease. Cytokines normally direct the immune system to sites of infection in the body and increase inflammation. However, in cytokine storm, excessive cytokines and inflammation leads to hyperinflammation that can cause damage to the tissues. The researchers propose that otherwise healthy patients who have a severe reaction to COVID-19 infection may be experiencing cytokine storm. The researchers analyze qPCR data from confirmed COVID-19 patients with mild…
- In the Fast Forward Box Visualizing X ChromosomeInactivation in Transgenic Mice, suppose the investigators had looked at the expression of green and red fluorescent protein in early mouse embryos, when theembryos have fewer than 500 cells. What patterns wouldthey likely have observed? (Assume that the transgenesmake gene product this early in development.)10- 00 O SCNSA-SCN10A CDKN1A HANDI VTI1A SYTI • MYOCD 9 10 11 12 13 14 15 16 17 18 19 20 2122 X YTwo adjacent cells in the nematode worm nor-mally differentiate into an anchor cell (AC) and a ventraluterine precursor (VU) cell, but which of the two becomesthe AC and which becomes the VU cell is completely ran-dom: the cells have an equal chance of adopting eitherfate, but they always adopt different fates. Mutations ofLin12 alter these fates. In hyperactive Lin12 mutants, bothcells become VU cells, while in inactive Lin12 mutants,both cells become ACs. Thus, Lin12 is central to the deci-sion-making process. In genetic mosaics in which oneprecursor cell has the hyperactive Lin12 and the other pre-cursor has the inactive Lin12, the cell with the hyperactiveLin12 always becomes the VU cell and the cell with inac-tive Lin12 always becomes the AC. Assuming that one cellsends a signal and the other cell receives it, explain howthese results suggest that Lin12 encodes a protein requiredto receive the signal. Offer a suggestion for how the fatesof these two precursor cells are normally…