If one DNA single strand has the sequence 5’-AATGCAA-3’, what is the sequence of its complementary strand?
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1. If one DNA single strand has the sequence 5’-AATGCAA-3’, what is the sequence of its complementary strand?
2. When
3. What are the types and major functions for each type of RNA?
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- 4. How is replication different from transcription in terms of product? 5. What do you call each triplet code made up of three linearly arranged nucleotides in the mRNA? 6. What is the complement of the mRNA triplet code in the tRNA? 7. In what way is tRNA different from mRNA? 8. In what ways are RNA and DNA similar? 9, In what ways are they different?1. Determine what amino acid will be formed from the given DNA strand below: 3’ T A C A T G C C G A A T G C C 5’ 2. how will replication happen by mentioning the enzyme needed then transcribe to form mRNA. 3. Discuss what will happen to mRNA, then translate, mentioning the anticodon to be used 4. Look at the genetic code to know what amino acid will become part of the polypeptide chain.1.What is corresponding amino acid chain of the mRNA sequence AUG-CGU-UCU-GCU GGU-UAG? 2. What are the tRNA anticodons of the mRNA codons from item 1? 3. What is the complementary DNA strand of the mRNA strand from item no 1? 4. The DNA strand from item 3 undergoes replication. What will be its complementary DNA strand?
- 1. What are the types and major functions for each type of RNA? 2. Define transcription and translation. Which process occurs first to make protein from DNA? 3. In what direction does a polymerase move when synthesizing a strand of mRNA?1. What are the differences between DNA and RNA? 2. Which process involves copying of DNA information from template strand and produces mRNA strand that will be used for protein synthesis? 3. Based on the activity, what is the function of anticodon(TRNA) on protein synthesis? 4. What causes mutation? Is it always harmful? 5. Does a simple change on DNA sequence affect the resulting protein? Explain briefly.1. When DNA replicates, how is it able to “unwind” its double helix? 2. Instead of the term “Formation of a nucleoside”, what could the name of the reaction be? What functional group is being formed? 3. Define the primary structure of DNA/RNA. Compare and contrast to the primary structure of proteins.
- 1. It is a large complex ribonucleoprotein particle that chemically modifies the pre mRNA strand by the removal of introns 2. This is a linkage between paired bases in the secondary DNA structure 3. It refers to the linkage between 2 nucleotides in the DNA molecule 4. Give the range of the number of nucleotides present in a single protein molecule 5. Identify the number of bases included in the nucleosome 6. It is an enzyme that cuts the bacterial plasmid at its cleavage site2. Suppose the following base sequence was found in a 20-base DNA polymer. C-A-G-T-T-A-A-G-G-T-C-C-T-A-G-G-T-T a. What would be the bases in the complementary strand of DNA? b. What would be the mRNA strand transcribed? c. What would be the corresponding tRNA anticodons? d. What are the amino acids coded by the transcribed mRNA?7. What is the total number of hydrogen bonds that exist between the DNA strand 5'-TTCAGAG-3' and its complementary strand? 8. What is the relationship of the new DNA chain of a daughter DNA double helix and the old DNA chain in the other daughter DNA double helix? 9. From what DNA base sequence was the following mRNA sequence transcribed? 5’ – UUCGAG—3’
- 1. What is the nucleotide sequence of the complementary strand of the DNA molecule: 5’-AATGCGATCTTCAT-3’?2. What is the nucleotide sequence of the mRNA transcribed from the template DNA strand: 3’-GCTACAAAAAGTCCATAATCGC-5’? Indicate the 5’ and 3’ ends.3. If the mRNA sequence you obtained in question 2 were to be translated, what would be the sequence of amino acids? Indicate the N-terminal and C-terminal amino acids.4. Which of the following single-stranded DNA molecules would be palindromic in the double-stranded state? (A-T-G-C-C-G-T-A, G-T-C-A-T-G-A-C, A-T-G-C-T-A-C-G, G-C-T-A-T-G-A-C)4. Within this double-stranded segment of DNA, there is a protein-coding region for a gene. 3'-AATATGACGTCAGTAGA- 5' CODING STRAND ||| || || | || ||||| || 5'-TTATACTGCAGTCA TCT-3' a. Identify on which strand the gene begins. Draw a box around the start codon. (1 pt) b. Which strand will RNA polymerase follow when it transcribes the gene? (.5 pts) c. In what direction will it transcribe? Draw an arrow above or below the DNA strands to indicate. (0.5 pts) d. Write the RNA sequence that would be transcribed below. (1 pt) 5'- - 3' e. Write the amino acid sequence that would be produced during translation (use the codon table below). (1 pt)