how to make 100ml of TBST buffer from 1X TBS (tris buffered saline solution) and 0.1% tween-20
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how to make 100ml of TBST buffer from 1X TBS (tris buffered saline solution) and 0.1% tween-20. I am just confused how to do the calculations when it is 1X TBS and not 10X TBS. Please show calculations
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- How much of 10,000x SYBR safe would you add to 50 ml to make a final concentration of 1x? V1= 0.005mL B.How will you set up the serial dilution? How many tubes do you need? What is the concentration in each? How much LB will you add to each tube? What volume of cells will you add?DILUTION COLONY COUNT CFU/mL 1:106 155000 1:107 15500 1:108 1550 1:109 155 Given these values how would I fill in the rest of this serial dilution table? Also, what would be a 1:1 CFU/mL value based on this table?4 mL of 10% TCA solution was added to 1 mL of serum and after mixing, it was waited for two minutes and filtered through non-phosphorus filter paper. 1 mL of the filtrate was taken and 13 mL of distilled water, 4 mL of sulfomolybdic acid and 2 mL of dilute SnCl2 solution were added and mixed, and after waiting for 15 minutes, the absorbances of the obtained solutions against pure water at 520 nm were read. If the function of the calibration graph obtained with 0.5-2.5 mg/mL standard phosphorus solutions is y= 0.245x + 0.107 and the absorbance value of the serum sample is 0.109, what is the amount of phosphorus in the sample?
- 3) With our 12.5 ug/mL solution, we will make serial dilutions with a dilution factor of 2. We want to make 1 mL of 1/2, 1/4, 1/8, 1/16, 1/32, and 1/64 dilutions. Draw a diagram describing how you will conduct the serial dilutions and insert it below. The 1/2 dilution (tube 2) will be made from the 12.5 ug/mL solution (tube 1). The 1/4 dilution (tube 3) will be made from the 1/2 dilution (tube 2LA pharmacist prepares 80 mL of an isotonic solution of 1.1 %w/v Lidocaine HCl (E-value 0.2). To prepare the solution, he dissolves Lidocaine HCl in purified water to make an isotonic solution. Then he dilutes the Lidocaine HCl solution with 0.9%w/v sterile sodium chloride to complete the formulation. How many milliliters of purified water is needed to make the formulation by this method?AE, 62, Male Wt: 75 kg, Ht: 150 cm Orders: Penicillin G Potassium 10,000,000 Units Sterile water for injection qs Make a 250,000 U/mL solution. The package insert states If Penicillin G Potassium 10,000,000 Units is reconstituted with 44 mL sterile water, a solution of 200,000 U per mL will be obtained. What volume of sterile water would you use to reconstitute the penicillin G potassium in order to make the ordered solution?
- Your pharmacy has on hand Tetracycline 250 mg/5 mL liquid.You receive a prescription for Tetracycline syrup 500 mg qid for 7 days. How many total milliliters would you dispense? Your pharmacy has on hand Prednisone 5 mg tablets.You receive a prescription for Prednisone 5 mg ii tabs stat and qid x 1 day, then ii tabs tid x 2 days, then ii tabs bid x 2 days, then i tab tid x 3 days, then i tab bid x 3days, then i tab qd x 4 days How many tablets would you dispense for this prescription? How many days will the patient take this medication for? Your pharmacy has on hand Dilantin 100mg capsules.You receive a prescription for Dilantin 100 mg 1 cap po in the AM & PM, 200mg (2 caps) po at noon, and 300mg (3 caps) po at hs. Dispense a 30 day supply. (using the 100mg capsules you have in stock) How many capsules will you dispense? Your pharmacy has on hand Dakins Irrigation Solution 1 liter.You receive a prescription for Dakins Irrigation Solution Irrigate chest tube with 100 mL…Choose the one answer that fits best. Which of the following statements regarding the proper procedure for using Micropipettes is NOT correct? O a. You cannot use a 2-20 µl micropipette to pipet 200 µl O b. To expel all the liquid from the tip, you have to press the eject button O c. To draw up solution, press and hold the plunger at the first stop before entering the solution O d. Micropipettes always require the use of a disposable plastic tip O e. While pipetting, micropipettes should always be held as straight as possibleWhat is the minimal concentration (MIC) for disinfectant number 1? Just write the corresponding number including the decimal as written. ug/mL. Disinfectant 1 2 3 4 concentration 0.1 ug/mL +++ ++++ ++ +++ 0.2 ug/mL ++ ++ + ++ 0.5 ug/mL + + 1.0 ug/mL 5.0 ug/mL subculture 1 2 3 4 0.1 ug/mL ++++ ++++ ++++ ++++ 0.2 ug/mL ++++ ++++ ++++ ++++ 0.5 ug/mL ++++ ++++ ++++ 1.0 ug/mL ++++ 5.0 ug/mL Submit response
- Now prepare a 500 ml media having ¼ strength MS with 5mM BAP, 5.5 mg/l Kn and coconut water 10%. Stocks are MS Macro 10X, micro 20X, FeEDTA 10X, Organic 100X, BAP and Kn 5gm/100ml. If you need anything else then you add accordingly. BAP has a MW of 225.3. Sucrose and Myo-inositol is not mentioned in the question. These you need to add. Consistency of the medium is also mentioned.What is your overall dilution factor if you complete 3 serial dilutions using a 100-fold dilution each time? (Show your work)Streptomycin sulfate 400 mg IM daily is ordered for a child weighing 77lbs. The recommended dosage is 20-40 mg/kg/day once daily. A 1 gram vial of streptomycin sulfate is available in powdered form with the following instructions: Dilution with 1.8 mL of sterile water will yield 400mg per mL. If safe, how many mLs will you give?