when v=vmax/2，Km =（S） 1.why？？Need Explaination. What is V and what is Vmax？how is their relationship and how they affect each other？Detail explanation please. The Fomular：V/E total = K cat. 2. What Is E total？？？need definition of E total.Detail explanation please
when v=vmax/2，Km =（S） 1.why？？Need Explaination. What is V and what is Vmax？how is their relationship and how they affect each other？Detail explanation please. The Fomular：V/E total = K cat. 2. What Is E total？？？need definition of E total.Detail explanation please
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
when v=vmax/2，Km =（S）
1.why？？Need Explaination.
What is V and what is Vmax？how is their relationship and how they affect each other？Detail explanation please.
The Fomular：V/E total = K cat.
2. What Is E total？？？need definition of E total.Detail explanation please.
![Significance of KM and Vmax
When V = Vmax/2, KM = [S]
Consider a case in which the reaction E +S →ES
takes place more frequently than ES → E + P
●
●
●
●
• k_₁ is greater than k₂ (k_₁ >> k₂)
is related to the turnover number of an enzyme
Turnover number: Number of moles of substrate that
react to form product per mole of enzyme per second
Also called Keat or kp
Vm
max
ved.
●
V
[E₁]
= turnover number = kat](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F46abcb61-b74c-47ad-92fe-fd35f6687cc0%2F4d20013a-f2f1-4da6-abea-9942ecfd97bc%2Fanx4rcn_processed.jpeg&w=3840&q=75)
Transcribed Image Text:Significance of KM and Vmax
When V = Vmax/2, KM = [S]
Consider a case in which the reaction E +S →ES
takes place more frequently than ES → E + P
●
●
●
●
• k_₁ is greater than k₂ (k_₁ >> k₂)
is related to the turnover number of an enzyme
Turnover number: Number of moles of substrate that
react to form product per mole of enzyme per second
Also called Keat or kp
Vm
max
ved.
●
V
[E₁]
= turnover number = kat
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
Step by step
Solved in 3 steps
Recommended textbooks for you
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON