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A broth tube is inoculated at 9 AM. At 12 PM there are 6,400 cells. At 2 PM there are 400,000 cells. How many cells were put in the tube?
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- Clarince inoculates a TSI Slant on Tuesday and one day later the tube is all yellow. She does not record the result until a week later when the tube is all red. What happened to cause the color change?Answer each of the questions below using the plate images provided. Nutrient Agar 1 EMB Blood Agar 1. How many colonies are on NA plate 3?. 2. How many bacteria were transferred to NA plate 3?. 3. How many colonies are on NA plate 2? 4. How many bacteria were transferred to NA plate 2?. 5. How would you describe the growth on NA plate 1?. 6. How many colonies grew together on NA plate 1? 7. How many bacteria were transferred to NA plate 1? Nutrient Agar 2 8. How many bacteria collected from the source? (Hint: What fraction of tube 1 was spread on NA plate 1?) 9. Were any of the bacteria from the source Gram-negative? How can you tell?. 10. Could any of the bacteria from the source ferment lactose? How can you tell? Nutrient Agar 3 11. Were any of the bacteria from the source hemolytic? How can you tell?. 00Which test tube is the control group? Test tube 1: 5 Hydrogen peroxide only Test tube 2: 5 Hydrogen peroxide and ground potato Test tube 3: 5 Hydrogen peroxide and diced potato Test tube 4: 5 Hydrogen peroxide And boiled potato
- A microbiologist sets up a serial dilution to analyze bacteria in raw milk. Tube 1 – 0.1 mL milk diluted in 9.9 mL sterile water Tube 2 – 0.1 mL milk from Tube 1 diluted in 9.9 mL sterile water Tube 3 – 1.0 mL milk from Tube 2 diluted in 9.0 mL sterile water What is the dilution of raw milk in Tube 3?If a broth tube is inoculated with 100 cells which immediately enter exponential growth, how many cells will there be after 8 hours if the doubling time is 30 minutes?A broth culture is initially inoculated with a single bacterial cell. For the first hour the bacteria has a generation time of 30 minutes, for the second hour the generation time has been reduced to 15 minutes, and for the third hour the generation time has been reduced to 10 minutes. At the end of the third hour how many bacteria are present in the broth culture?
- A broth containing 1 million bacterial cells is treated with an antimicrobial agent that kills 90% of the cells in 1 minute. What is the minimum amount of time it will take before all the cells in the broth are dead? 3 minutes 5 minutes 7 minutes 10 minutesAs you can see on the blood agar plates, some microbes can lyse red blood cells releasing nutrients into the surrounding area. What might the advantage be for a bacteria to be able to do this? Do you think this could have an effect on the bacteria’s virulence? Explain.A culture with approximately 4x105 cells/mL were incubated. After 10 hours, the number of cells had increased to 5x109. a) How long was the generation time in minutes?b) How many generations have occurred?
- Jessie is working with a 100% stock concentration of Clorox. She decides to add 100 μL of bacteria to atube containing 3 mL of nutrient broth and then adds 400μL of the Clorox stock to the tube as well. Whatis the unknown concentration of the tube?The PD# of your culture is 165. You plated 3745 cells per well. You have recovered 12,655 cells. What's the new PD#?Explain why the following steps are essential during subculturing:a. Flaming the inoculating instrument prior to and after each inoculation.b. Holding the test tube caps in the hand as illustrated in Figure 1.1 on page 24.c. Cooling the inoculating instrument prior to obtaining the inoculum.d. Flaming the neck of the tubes immediately after uncapping and beforerecapping