Is the Peptone Iron Agar (PIA) a complex or defined medium? Explain based on its composition. Is the test tube a A) broth, B) slant, or C) deep agar medium? Why is a "needle" used to inoculate?
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Depending on their composition, media can be classified as defined and undefined media. Defined media are also called chemical media.
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- Is the Triple-Sugar Iron Agar (TSIA) a complex or defined medium? Explain based on its composition. Is the test tube a A) broth, B) slant, or C) deep agar medium? Why is a “needle” used to inoculate?Not only is blood agar an enriched medium that can support fastidious organism growth, but it can also serve as a differential medium in the identification of streptococcal species because of the presence of red blood cells that can be destroyed by an organism's hemolysins. Looking at this photo, how could you describe the organism growing on the surface of this blood agar plate? Multiple Choice a) Gamma-hemolytic b) Alpha-hemolytic c) Non-hemolytic d) Beta-hemolyticThe Petri Dish method is used in microbiology to raise bacteria in: a) rapid growth b) pure culture c) septic environment d) all of the above 2. What is the difference between antiseptic and sanitization?3. In order to prevent any kind of contamination the medium must be _________ before placing it in the Petri dish. a) lyophilized b) pasteurized c) autoclaved d) distille
- About becterial enzymes, it is CORRECT to affirm that: a). Some becterial enzymes can help the microbe fight the immune system, by destroying lgA.b). Coagulases are enzymes that use the host's fibrinogen and form a semi-solid get that isolates amd protects the bacteria.c). Some bacterial enzymes such as hyaluronidase can help the microble move through the host's tissus by destroying the tissue's components.d). Fibrolysin is an enzymes that can help from blood clots.A culture of Staphylococcus is diluted as follows:(1) 20mL are added to 80mL of water.(2) 10uL from (1) are then added to 9.99mL of water.(3) A 10-2 dilution is made from tube # (2).(4) 100uL from (3) are plated for a pour plate and incubated. There were 34 colonies counted on one quarter of the plate following incubation. a) What was the overall dilution?b) How many cfu/mL were present in the original culture?Which microbe requires serum components to be added to the growth medium?
- You mixed up the numbers on the tubes when you inoculated the mannitol salt agar (MSA) plate. You do not know if you grew staph epidermis or E. coli. You found that the organism growing on the mannitol salt agar remained red after incubation. It is most likely that the organism is E.coli. a) True b) FalseWhich test require a straight line inoculation? Choose all that apply. A) Bile esculinase B) NaCl C) DNA hydrolysis D) HemolysinA) Draw and label the set-up of Manual paraffin wax infiltration. B) Answer the following questions:1. Differentiate the 3 types of tissue Impregnation in terms of: 2. Impregnation Techniques Advantages Disadvantages A. Paraffin Infiltration B.Celloidin Infiltration C.Gelatin Impregnation C) What is the required volume of embedding medium for routine tissue processing? D) What is Plastic embedding? What is Double embedding?
- If the pH indicator were left out of MacConkey agar, the medium would bea) complex.b) differential.c) defined.d) defined and differential.e) complex and differential.N Please observe the following cells that have been Gram stained. "* 3 E ER $ JO 4 R % 5 6 F G & Y I U 8 00Write a paragraph explaining the Gram stain result and the differences in the endospores of these three spices: a) Clostridium perfringes b) Clostridium tetani C) Clostridium botulinum