Determine what percentage of the culture was living (viable) and what percentage was dead (mortality). PlatesPlate DilutionVolume platedNo.of coloniesAvg NoConcentration of diluted sample Cd(cells/mL)Concentration of original sample Cu(cells/mL)110^-310ulR1=130,R2= 110,R3=210150150mL1.50*10^6Volume of cells(mL)Volume of diluent(mL)Total dilution(D)Hemocytometer count Avg cells in 1 mm^2 areaConcentration of diluted sample Cd(cells/mL)Concentration of original sample Cu(cells/mL)4.30.50.1grid 1= 171 , grid2 = 1851781.78*10^51.78*10^6

The word "CFU" in microbiology refers to a colony-forming unit, which is used to estimate how many…

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Human Anatomy & Physiology (11th Edition)

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ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

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Chapter1: The Human Body: An Orientation

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Determine what percentage of the culture was living (viable) and what percentage was dead (mortality).

Plates	Plate Dilution	Volume plated	No.of colonies	Avg No	Concentration of diluted sample Cd(cells/mL)	Concentration of original sample Cu(cells/mL)
1	10^-3	10ul	R1=130,R2= 110,R3=210	150	150mL	1.50*10^6

Volume of cells(mL)	Volume of diluent(mL)	Total dilution(D)	Hemocytometer count	Avg cells in 1 mm^2 area	Concentration of diluted sample Cd(cells/mL)	Concentration of original sample Cu(cells/mL)
4.3	0.5	0.1	grid 1= 171 , grid2 = 185	178	1.78*10^5	1.78*10^6

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