Derive the Scatchard equation for a reaction system 2P + X ↔ P2X, in which only P and P2X have measurable absorbances at the chosen wavelength. Based on this equation and the data given below, determine the values of Keq and ??2? (epsilon P2X). Assume the width of the cuvet used in all of these measurements to be 1cm, i.e., b = 1cm,and the absorbance of the blank solution to be zero.Sample#P0 (M)X0 (M) A14×10-400.3122×10-42×10-20.3734×10-44×10-20.79

Throughout this article, [RL] denotes the concentration of a receptor-ligand complex, [R] the…

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Macroscale and Microscale Organic Experiments

7th Edition

ISBN:9781305577190

Author:Kenneth L. Williamson, Katherine M. Masters

Publisher:Kenneth L. Williamson, Katherine M. Masters

Chapter14: Ultraviolet Spectroscopy, Refractive Indices, And Qualitative Instrumental Organic Analysis

Section: Chapter Questions

Problem 4Q

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Derive the Scatchard equation for a reaction system 2P + X ↔ P₂X, in which only P and P₂X have measurable absorbances at the chosen wavelength. Based on this equation and the data given below, determine the values of K_eqand ??2? (epsilon P₂X). Assume the width of the cuvet used in all of these measurements to be 1cm, i.e., b = 1cm,and the absorbance of the blank solution to be zero.

Sample#	P₀ (M)	X₀ (M)	A
1	4×10^-4	0	0.31
2	2×10^-4	2×10^-2	0.37
3	4×10^-4	4×10^-2	0.79

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